Addgene

We narrowed to 2,422 results for: gem

Showing: 1881 - 1900 of 2422 results

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pRU46

Plasmid

#167668

Purpose

ENTRY vector for gRNA6 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU48

Plasmid

#167670

Purpose

ENTRY vector for gRNA8 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU47

Plasmid

#167669

Purpose

ENTRY vector for gRNA7 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBP-lldR

Plasmid

#74095

Purpose

L-lactate repressor (LldR) from E. coli MG1655

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

lldR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBP-T7RNAP

Plasmid

#74096

Purpose

T7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNA

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

T7 RNAP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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HBV 1.3-mer P-null replicon

Plasmid

#65462

Purpose

containing 1.3 units of the P-null HBV genome (subtype ayw)

Depositor

Wang-Shick Ryu

Article

Wang et al J Virol. 2009 Jun;83(11):5815-24. doi: 1

Insert

1.3 units of the P-null HBV genome

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pSFVCs-lacZ

Plasmid

#92076

Purpose

This is a high level expression plasmid for production of recombinant genomic SFV RNA encoding a C-beta galactosidase fusion protein.

Depositor

Henrik Garoff

Article

Sjoberg et al Biotechnology (N Y). 1994 Nov;12(11):112

Insert

C-lacZ

Use

C-lacz expression plasmid, for run-off transcript…

Tags

Expression

Mutation

Promoter

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pPLP1.5

Plasmid

#22614

Depositor

Lynn Hudson

Article

Puckett et al J Neurosci Res. 1987 . 18(4):511-8.

Insert

human Proteolipid Protein cDNA (PLP1 Human)

Use

E. coli phagemid vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pTU1-A-RFP

Plasmid

#72939

Purpose

Level 1 Destination vector - Position A

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 1 - A site

Use

Synthetic Biology

Tags

Expression

Mutation

Beta-lactamase - G717C (nucleotide) - silent muta…

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSLI-TGD(1463000)

Plasmid

#85791

Purpose

P. falciparum integration plasmid to disrupt genes using SLI

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

PF3D7146300 targeting region, GFP-2TA-Neo-R

Use

P. falciparum genomic integration (gene disruptio…

Tags

GFP

Expression

Mutation

Promoter

none

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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p177bp-LacOx162-Hyg

Plasmid

#210215

Purpose

Integrates 162xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

162xLacO repeats derived from: PMID: 12864855.

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pG-HIV-2LTR

Plasmid

#104590

Purpose

standard for qPCR

Depositor

Kristine Yoder

Article

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

2LTR circle junction of HIV-1

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pTC150

Plasmid

#70014

Purpose

'Donor only' control plasmid for gene targeting of tomato ANT1 gene. Contains donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Donor + GVR

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pMB1-A-ptet-dCas9

Plasmid

#190129

Purpose

Level 1 MoClo plasmid containing an aTC inducible dCas9 for CRISPRi

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

dCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBP-SJM901

Plasmid

#72966

Purpose

Level 0 - SJM901 promoter (5' CTAT/3' GTAC fusion)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

SJM901 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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DT035A

Plasmid

#159397

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tac promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.tac-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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p177bp-LacOx50-Hyg

Plasmid

#210213

Purpose

Integrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMB1-A-pBAD-dCas9

Plasmid

#190132

Purpose

Level 1 MoClo plasmid containing an arabinose inducible dCas9 for CRISPRi

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

dCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSkipFlox

Plasmid

#85797

Purpose

P. falciparum plasmid for expression of both diCre fragments from single casette using skip peptide

Depositor

Tobias Spielmann

Article

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

split Cre recombinase

Use

Cre/Lox; P. falciparum expression

Tags

FKBP, FRB*

Expression

Mutation

Promoter

Pfcrt

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pG-HIV-LRT

Plasmid

#104592

Purpose

Standard for qPCR

Depositor

Kristine Yoder

Article

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

HIV-1 late reverse transcripts

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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