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pCPP5372

Plasmid

#128733

Purpose

Gateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal HA tag

Depositor

Alan Collmer

Article

Oh et al J Bacteriol. 2007 Nov;189(22):8277-89. E

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pBbA2c-RFP

Plasmid

#35326

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Tet

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pBad33_ISDge10_reRNA

Plasmid

#205340

Purpose

Bacterial expression of ISDge10 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISDge10 TnpB

Expression

Bacterial

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pOSIP-CH (CamR, HK022)

Plasmid

#45980

Depositor

Drew Endy, Keith Shearwin

Article

St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only

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pKM492-Myc-His

Plasmid

#109283

Purpose

ORBIT integrating plasmid for C-terminal tagging with Myc-His tag

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

attB-Myc-His

Use

Orbit integrating plasmid

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pREXC3GH

Plasmid

#47077

Purpose

FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Lactococcus lactis expression

Tags

3C protease site, GFP, and His

Promoter

NIS (nisin)

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-C-Myc-DDK-IRES-Puro METTL3

Plasmid

#200724

Purpose

Lentiviral expression of Flag-Tagged human METTL3

Depositor

Claudio Alarcón

Insert

METTL3 (METTL3 Human)

Use

Lentiviral

Tags

Myc-DDK from backbone plasmid

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGT-Ah9

Plasmid

#122576

Purpose

MAGIC donor plasmid (Himar transposon with tetQ/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

tetQ/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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SeAvs3

Plasmid

#188791

Purpose

Avs3 from Salmonella enterica NCTC13175

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

SeAvs3

Expression

Bacterial

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pGE144 (MutaEco; aTC-induced)

Plasmid

#189824

Purpose

Expresses MutaEco for generating mutations in the E. coli genome (aTC-induced)

Depositor

Seokhee Kim

Article

Eom et al Nucleic Acids Res. 2022 Apr 22;50(7):e38

Insert

EcoRNAPα-PmCDA1

Expression

Bacterial

Promoter

tetR/tetA promoter

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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