We narrowed to 2,390 results for: D10

Showing: 1901 - 1920 of 2390 results

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pGFP-mTet1s pc3901

Plasmid

#201700

Purpose

Expresses of GFP-tagged mTet1s in mammalian cells

Depositor

Cristina Cardoso

Article

Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

Insert

Tet1s (Tet1 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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Cbh_v5 AAV-saCBE N-terminal

Plasmid

#137182

Purpose

AAV genome: expresses the N-terminal of S. aureus v5 AAV-CBE from the Cbh promoter, U6-sgRNA

Depositor

David Liu

Article

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-saCBE N-terminal

Use

AAV

Mutation

Cas9 D10A

Promoter

Cbh

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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miniCGBE1-NG (pBM1182)

Plasmid

#140257

Purpose

CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

BE4max(R33A)_NG-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pEX-SP-YFP-STIM1(D76A)

Plasmid

#18859

Depositor

Tobias Meyer

Article

Liou et al Curr Biol. 2005 Jul 12. 15(13):1235-41.

Insert

Stromal interaction molecule 1 (STIM1 Human)

Tags

YFP

Expression

Mammalian

Mutation

D76A mutation - changed aspartate 76 to alanine. …

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only
ABE8.13-m

Plasmid

#136296

Purpose

expresses ABE8.13-m in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.13-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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miniABEMax V82G nSaCas9

Plasmid

#135365

Purpose

Expression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitution

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

miniABEMax V82G nSaCas9

Use

CRISPR

Expression

Mammalian

Mutation

(V82G)

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_POLD2-APOBEC1-UdgX-nCas9-UdgX

Plasmid

#163543

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

POLD2-APOBEC1-UdgX-nCas9-UdgX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDONOR223 RpH-LAMP1-3xFLAG

Plasmid

#163019

Purpose

gateway donor vector encoding ratiometric sensor of lysosomal lumenal pH

Depositor

Massimiliano Stagi

Article

Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

Insert

RpH-LAMP1-3xFLAG (Lamp1 Mouse)

Use

Gateway system

Tags

3XFLAG

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_eA3A-NG-nCas9

Plasmid

#163536

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

eA3A-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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LAMP-mChF-AIP

Plasmid

#61524

Purpose

Expression of Homo sapiens lysosomal-associated membrane protein 1 (LAMP1) tagged with mCherry and AIP

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

lysosomal-associated membrane protein 1 (LAMP1 Human)

Tags

AIP and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-CR033

Plasmid

#234042

Purpose

expresses ABE8e(V106W) base editor in a human T cell optimized lentiviral transfer plasmid

Depositor

Alexander Marson

Article

Schmidt et al Nature. 2024 Jan;625(7996):805-812. doi:

Insert

TadA8e(V106W)-Cas9(D10A)-P2A-blast

Use

Lentiviral

Available Since

April 4, 2025

Availability

Academic Institutions and Nonprofits only

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pT2-hUbC-dCas9-KRAB-T2a-Puro

Plasmid

#225999

Purpose

To establish stable dCas9-KRAB knock-in line

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

humanized dCas9-KRAB T2A Puro

Use

Sleeping beauty transposon

Expression

Mammalian

Mutation

D10A and H840A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF0724

Plasmid

#143070

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NFKB2 (NFKB2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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Str-STIM1-NN_neomycin

Plasmid

#65311

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

STIM1 mutated for binding motif to microtubule (STIM1 Human)

Tags

streptavidin

Expression

Mammalian

Mutation

2 amino acids of STIM1 (IP from SxIP motif) were …

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV_Anc689-NG-nCas9

Plasmid

#163527

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

Anc689-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET-A3A(N57Q)-BE3

Plasmid

#158157

Purpose

T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS

Depositor

Keith Joung

Article

Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10

Insert

6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS

Tags

6xHis tag on N-terminus

Expression

Bacterial

Promoter

T7

Available Since

Nov. 14, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-CAD

Plasmid

#73566

Purpose

Soluble red fluorescent activator of Orai1 channels

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

mCherry-CAD (STIM1 342-448) (STIM1 Synthetic, Human)

Tags

6x His and mCherry

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-mCherry-LAMP1

Plasmid

#222318

Purpose

Synchronize the trafficking of LAMP1 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP1 fused to SBP-mCherry (LAMP1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-2X-P2A-Puro

Plasmid

#110840

Purpose

Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-2X

Use

Lentiviral

Mutation

D10A and 2 tandem NLS sequences in the XTEN linker

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pRS0045

Plasmid

#131124

Purpose

Mammalian Target-ACE expression vector

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI

Use

CRISPR

Tags

PmCDA1, TadA, and UGI

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

Promoter

CMV promoter

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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