We narrowed to 2,231 results for: D10

1. pENTR1A mKate2-hu_ncOGT K842M

   Plasmid

   #154284

   Purpose

   Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Gateway entry vector

   Tags

   2x FLAG and mKate2

   Mutation

   K842M mutation (Catalytic Inactive)

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. SPACE-VRQR (pRZ5137)

   Plasmid

   #140245

   Purpose

   CMV promoter expression plasmid for TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   SPACE_VRQR
   

   Expression

   Mammalian

   Mutation

   V82G in TadA*, VRQR mutations in SpCas9, D10A in …

   Promoter

   CMV

   Available Since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCI-Puro_mouse_Adar2_Tv1

   Plasmid

   #158111

   Purpose

   expresses mADAR2 shortest transcript, puromycin selection

   Depositor

   Emanuela Felley-Bosco

   Article

   Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

   Insert

   adar2 (Adarb1 Mouse)
   

   Expression

   Mammalian

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. LAMP2-bio-His

   Plasmid

   #51861

   Purpose

   Expresses full-length Lysosome-associated membrane glycoprotein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   LAMP2 (LAMP2 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. SPACE-ΔUGI (pRZ1836)

   Plasmid

   #140243

   Purpose

   CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   SPACE-ΔUGI
   

   Expression

   Mammalian

   Mutation

   V82G in TadA*, D10A in Cas9, R187W in pmCDA1

   Promoter

   CMV

   Available Since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

   Plasmid

   #208944

   Purpose

   A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEX-YFP-STIM1-deltaK

   Plasmid

   #18861

   Depositor

   Tobias Meyer

   Article

   Liou et al Proc Natl Acad Sci U S A. 2007 May 29. 1

   Insert

   STIM1 (STIM1 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Stop codon inserted at amino acid postion 671 of …

   Available Since

   Oct. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-xBE3-P2A-Puro

   Plasmid

   #110870

   Purpose

   Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)-BE3
   

   Use

   Lentiviral

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR1A mKate2-hu_ncOGT D554N

   Plasmid

   #154283

   Purpose

   Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; D554N variant, no Ser/Thr glycosylation)

   Depositor

   Suzanne Walker

   Article

   Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Gateway entry vector

   Tags

   2x FLAG and mKate2

   Mutation

   D554N mutation (Ser/Thr Glycosylation Impaired)

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. CGBE1-VRQR (pBM1192)

    Plasmid

    #140254

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_VRQR-ΔUGI
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR1A mKate2-hu_ncOGT 5N5A

    Plasmid

    #154285

    Purpose

    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; 5N5A variant, no HCF-1 cleavage)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Mutation

    N322A, N356A, N390A, N424A, N458A mutations (HCF-…

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEX-CMV-SP-YFP-STM1(24-685) EF hand 3 mut (A79K, N80D, D82G)

    Plasmid

    #18865

    Depositor

    Tobias Meyer

    Article

    Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

    Insert

    Stromal interaction molecule 1 (STIM1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    3 mutations (A79K, N80D, D82G) in the EF hand of …

    Available Since

    March 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCC_06 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid

    #139091

    Purpose

    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    dSpCas9-NG-VPR
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1)

    Plasmid

    #26797

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    EF1a-tTA/TetO-mCherry-P2A-Rs1 (HTR4 Human)
    

    Expression

    Mammalian

    Mutation

    Human 5HT4B receptor with D100A mutation, generat…

    Available Since

    Feb. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNOC_dCas9_sgRNA NC

    Plasmid

    #176259

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Mutation

    H840A and D10A mutations on spCas9 to inactivate …

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEX-CFP-STIM1-deltaK

    Plasmid

    #18860

    Depositor

    Tobias Meyer

    Article

    Liou et al Proc Natl Acad Sci U S A. 2007 May 29. 1

    Insert

    STIM1 (STIM1 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Stop codon at amino acid position 671 of STIM1. C…

    Available Since

    Oct. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. LAMP1-bio-His

    Plasmid

    #51809

    Purpose

    Expresses full-length Lysosome-associated membrane glycoprotein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    LAMP1 (LAMP1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLAG-KIF5A

    Plasmid

    #166954

    Purpose

    Expresses FLAG-tagged KIF5A protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KIF5A (Kif5a Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pD649-HAsp-SEMA4A-COMP5AP-AviTag-9xHis

    Plasmid

    #157502

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SEMA4A (SEMA4A Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. attB-GFP-Tet1-Poly(A)

    Plasmid

    #65559

    Purpose

    Plasmid for Bxb1-mediated recombination of the GFP-Tet1 cDNA into a MIN-tagged locus

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Tet1 (Tet1 Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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