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pAMPK alpha2 delta312X

Plasmid

#60127

Purpose

expresses constitutively active AMPKalpha2

Depositor

Morris Birnbaum

Insert

AMPK alpha 2 (Prkaa2 Rat)

Tags

Myc

Expression

Mammalian

Mutation

Truncated at residue 312

Available since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcdna6.2- myc ULK1 k46I

Plasmid

#27630

Depositor

Reuben Shaw

Publication

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

mouse myc ULK1 K46I (Myc Mouse)

Tags

myc

Expression

Mammalian

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Available since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pAAV-FLEX-SaCas9-U6-sgSlc17a6

Plasmid

#124847

Purpose

Mutagenesis of Slc17a6 with SauCas9

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a6 gRNA (Slc17a6 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET-(-30)GFP-9xGGS-Cre-6xHis

Plasmid

#62372

Purpose

Expression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(-30)GFP-9xGGS-Cre-6xHis

Tags

(-30)GFP and 6xHis tag

Expression

Bacterial

Promoter

T7

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-ERas

Plasmid

#13355

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

ES cell expressed Ras (Eras Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA-KIRIN1-GR

Plasmid

#107418

Purpose

Mammalian cell expression plasmid for potassium ion ratiometric indicator KIRIN1-GR

Depositor

Robert Campbell

Publication

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1-GR

Expression

Mammalian

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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YFP NLS Beta-Actin S14C

Plasmid

#60614

Purpose

Encodes YFP and NLS tagged beta-actin with the S14C polymerization mutation

Depositor

Primal de Lanerolle

Publication

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

S14C

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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HcKCR1_mCherry_pcDNA3.1

Plasmid

#177336

Purpose

Expresses KCR1 in mammalian cells

Depositor

John Spudich

Publication

Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

Insert

HcKCR1

Tags

mCherry

Expression

Mammalian

Mutation

mammalian codon-adapted

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pC0051-W85X REPAIR targeting guide cloned into pC0043

Plasmid

#103867

Purpose

Guide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038

Depositor

Feng Zhang

Publication

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

W85X targeting guide RNA

Use

CRISPR

Expression

Mammalian

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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FUW TetO HA-AMPKa1 WT

Plasmid

#69824

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Publication

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

minimal CMV promoter with tetracycline operator

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-EGFP/RFP-int

Plasmid

#19818

Depositor

Zuoshang Xu

Publication

Qiu et al BMC Biotechnol. 2008 Oct 7;8:77. doi: 10

Type

Empty backbone

Use

Cre/Lox and RNAi

Expression

Mammalian

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pCDF-MCM4/6

Plasmid

#116951

Purpose

Expresses human MCM4 and human MCM6

Depositor

James Chong

Publication

Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

Inserts

Human MCM6 (MCM6 Human)
Human MCM4 (MCM4 Human)

Tags

His6

Expression

Bacterial

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-C1-CAMKIIalpha-T286A

Plasmid

#21220

Depositor

Tobias Meyer

Publication

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Insert

CAMKIIalpha-T286A (Camk2a Rat)

Tags

GFP

Expression

Mammalian

Mutation

T286A (Lab plasmid KS 0003).

Available since

July 29, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET15b SARS-CoV PLpro

Plasmid

#61693

Purpose

inducible expression of SARS PLpro protease

Depositor

Michael Johnson

Publication

Lee et al ChemMedChem. 2013 Aug;8(8):1361-72. doi:

Insert

SARS-CoV PLpro

Tags

His

Expression

Bacterial

Promoter

T7

Available since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0

Plasmid

#50022

Purpose

expresses Membrane tagged G-CaMP3 in cells expressing Cre

Depositor

David Anderson

Publication

Vrontou et al Nature. 2013 Jan 31;493(7434):669-73. do

Insert

G-CaMP3

Use

AAV

Tags

MARCKS sequence (MGCCFSKT)

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST-ORF-V2

Plasmid

#73638

Purpose

Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).

Depositor

Darren Saunders

Publication

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 2 (V2)

Expression

Mammalian

Promoter

CMV

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pDEST-ORF-V1

Plasmid

#73637

Purpose

Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 1 (V1)

Depositor

Darren Saunders

Publication

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 1 (V1)

Expression

Mammalian

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pM-Cas9

Plasmid

#196325

Purpose

35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP

Depositor

Zhenghe Li

Publication

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV M antigenome encoding SpCas9 in place of viral GP

Use

CRISPR

Tags

3×flag

Expression

Plant

Mutation

See depositor comments below

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAtetO_6xHis-Bst-LF

Plasmid

#145799

Purpose

Plasmid for the aTc-inducible expression of the large-fragment of Bst DNAP

Depositor

Andrew Ellington

Publication

Bhadra et al mSphere. 2021 May 19;6(3):e00911-20. doi

Insert

Bst-LF

Tags

6xHis

Expression

Bacterial

Promoter

tet PA

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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AAV2-hSyn-G-Flamp1

Plasmid

#188569

Purpose

green biosensor for cAMP in neurons

Depositor

Jun Chu

Publication

Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

Insert

G-Flamp1

Use

AAV

Tags

His tag

Promoter

hSyn

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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