We narrowed to 12,673 results for: sequence

1. EF1a_MESP1_P2A_Hygro_Barcode

   Plasmid

   #120459

   Purpose

   Barcoded lentiviral vector to express MESP1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MESP1 (MESP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV_PP1-mCitrine

   Plasmid

   #172472

   Purpose

   Mammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.

   Depositor

   Sean R Collins

   Article

   Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

   Insert

   prolactinSS-PP1-mCitrine (parapinopsina Zebrafish)
   

   Use

   Lentiviral

   Tags

   mCitrine and prolactin signal sequence (cleaved p…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Sept. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pASPIre3

   Plasmid

   #154844

   Purpose

   Derivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this study

   Depositor

   Markus Jeschek

   Article

   Hollerer et al Nat Commun. 2020 Jul 15;11(1):3551. doi:

   Insert

   Bxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAF0286 Ef1a-Cp36_attB-BFP acceptor

   Plasmid

   #193474

   Purpose

   Acceptor plasmid for 3 plasmid recombination assay

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Cp36_attB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.2 mir-1-1 reporter hsa-mir-133a-1

   Plasmid

   #46676

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   hsa-mir-133a-1 (MIR133A1 Human)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.2 mir-1-1 reporter hsa-mir-122

   Plasmid

   #46673

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   hsa-mir-122 (MIR122 Human)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. SRSF9_2xRRM_pGEX

   Plasmid

   #135120

   Purpose

   Recombinant expression of SRSF9 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   SRSF9 (SRSF9 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. prk5-MFSD12-TEV-3xFLAG-3xHA

   Plasmid

   #149504

   Purpose

   Transient expression of human MFSD12 with recoded nucleotide sequence.

   Depositor

   David Sabatini

   Article

   Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

   Insert

   MFSD12 (MFSD12 Human)
   

   Use

   Transient expression

   Tags

   TEV-3XHA-3XFLAG

   Expression

   Mammalian

   Mutation

   Codon Optimized Sequence

   Promoter

   CMV

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. SERPINA-bio-His

   Plasmid

   #52182

   Purpose

   Expresses full-length Alpha-1-antitrypsin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   SERPINA (SERPINA1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. SRSF8_RRM_pGEX

    Plasmid

    #135119

    Purpose

    Recombinant expression of SRSF8 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    SRSF8 (SRSF8 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGZ173 (pFA6a-SL-MNase-kanMX6)

    Plasmid

    #70234

    Purpose

    ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope

    Depositor

    Steven Henikoff

    Article

    Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

    Insert

    Short linker and aa 83-231 of Mnase
    

    Use

    Yeast genomic targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGG-TurboID-GFP-PTS1

    Plasmid

    #209406

    Purpose

    Transiently expressing and visualizing the subcellular localization of TurboID fused with a peroxisomal targeting signal type I (PTS1) in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    3XHA-TurboID-PTS1
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. EF1a_ASCL5_P2A_Hygro_Barcode

    Plasmid

    #120430

    Purpose

    Barcoded lentiviral vector to express ASCL5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    ASCL5 (ASCL5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. evoCas9

    Plasmid

    #138558

    Purpose

    Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    evoCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    M495V, Y515N, K526E, R661Q

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. A2M-bio-His

    Plasmid

    #53423

    Purpose

    Expresses full-length Alpha-2-macroglobulin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    A2M (A2M Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-Flag-dSpCas9

    Plasmid

    #92113

    Purpose

    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAVA-LGF2-Gal11p

    Plasmid

    #127481

    Purpose

    Positive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interaction

    Depositor

    Joel Malek

    Article

    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

    Insert

    LGF2-Gal11p (GAL11 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFX-β-tubulin-Venus

    Plasmid

    #175828

    Purpose

    Express Venus with microtubule-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Venus with microtuble-targeting sequence
    

    Use

    Tags

    human tubulin beta 4B class IVb;TUBB4B

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. EF1a_POU1F1_P2A_Hygro_Barcode

    Plasmid

    #120473

    Purpose

    Barcoded lentiviral vector to express POU1F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    POU1F1 (POU1F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACYC-Cas12g1-noncoding

    Plasmid

    #120880

    Purpose

    Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Noncoding sequences for CRISPR-Cas12g1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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