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pDONR221-ZNF665

Plasmid

#101448

Purpose

Donor Vector containing ZNF665 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF665 (ZNF665 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-S (P.1 lineage / Brazilian variant)

Plasmid

#169847

Purpose

Mammalian expression plasmid for S of SARS-CoV-2 (P.1 lineage / Brazilian variant) with N-terminal c-myc tag

Depositor

Erik Procko

Article

Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d

Insert

Protein S

Tags

HA leader and c-myc tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-S (B.1.1.7 lineage / UK variant)

Plasmid

#169848

Purpose

Mammalian expression plasmid for S of SARS-CoV-2 (B.1.1.7 lineage / UK variant) with N-terminal c-myc tag

Depositor

Erik Procko

Article

Zhang et al Nat Chem Biol. 2022 Mar;18(3):342-351. d

Insert

Protein S

Tags

HA leader and c-myc tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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Cavin4a-mCherry

Plasmid

#176010

Purpose

Mammalian expression of zebrafish Cavin4a-mCherry. Parton lab clone HYM

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4a (cavin4a Zebrafish)

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pKHH030_sgCtrl#2

Plasmid

#174143

Purpose

Lentiviral vector expressing a control sgRNA that targets a safe harbor site

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF879

Plasmid

#101623

Purpose

Donor Vector containing ZNF879 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF879 (ZNF879 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF646

Plasmid

#101625

Purpose

Donor Vector containing ZNF646 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF646 (ZNF646 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF735

Plasmid

#101595

Purpose

Donor Vector containing ZNF735 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF735 (ZNF735 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF587B

Plasmid

#101621

Purpose

Donor Vector containing ZNF587B transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF587B (ZNF587B Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF222

Plasmid

#101622

Purpose

Donor Vector containing ZNF222 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF222 (ZNF222 Human)

Use

Gateway shuttling vector

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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