We narrowed to 5,913 results for: pcDNA 3.1

1. pcDNA3.1-FLAG-SBP-STAG2 (S653X)

   Plasmid

   #73965

   Purpose

   STAG2 cDNA (CCDS43990) S653X

   Depositor

   Todd Waldman

   Article

   Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

   Insert

   STAG2 (STAG2 Human)
   

   Use

   Tags

   FLAG-SBP

   Expression

   Mammalian

   Mutation

   S653X

   Promoter

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1 2xFLAG-2xSTREP-Emsy (mouse)

   Plasmid

   #236419

   Purpose

   transient overexpression of EMSY in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

   Insert

   EMSY (2210018M11Rik Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1 2xFLAG-2xSTREP-EMSY (human)

   Plasmid

   #236420

   Purpose

   transient overexpression of EMSY in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

   Insert

   EMSY (EMSY Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1 2xFLAG-2xSTREP-Keap1 (mouse)

   Plasmid

   #236421

   Purpose

   transient overexpression of KEAP1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

   Insert

   KEAP1 (Keap1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1 2xFLAG-2xSTREP-KEAP1 (human)

   Plasmid

   #236422

   Purpose

   transient overexpression of KEAP1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

   Insert

   KEAP1 (KEAP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

   Plasmid

   #238007

   Purpose

   300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.

   Depositor

   Agnel Sfeir, Simon Powell

   Article

   Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:

   Insert

   AAVS1 (AAVS1, PPP1R12C Human)
   

   Use

   Rna donor to study rt-dsbr

   Tags

   Expression

   Mammalian

   Mutation

   3bp insertion signature (ATC) at Cas9 cut site in…

   Promoter

   CMV

   Available since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-lk-HiBiT

   Plasmid

   #233064

   Purpose

   Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. Also includes a C-terminal HiBiT tag.

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Use

   Tags

   ALFA and HiBiT

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G

   Plasmid

   #233063

   Purpose

   Expresses cleavage-deficient mouse ADGRL3-T923G (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Use

   Tags

   ALFA and HiBiT

   Expression

   Mammalian

   Mutation

   Threonine 923 to Glycine

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1 Cas9-P2A-dTomato-PS9

   Plasmid

   #231911

   Purpose

   For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'PS9' packaging signal

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   Cas9-P2A-dTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 Cas9-P2A-dTomato-T20

    Plasmid

    #231912

    Purpose

    For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'T20' packaging signal

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    Cas9-P2A-dTomato
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1 T21R-Nb139-T2A-mCherry

    Plasmid

    #225589

    Purpose

    Mammalian expression vector for TRIM21 RING-Nb139 anti-p53 degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    T21R-Nb139-T2A-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-myc:Skp1(dC111):gs10:FRB:HA

    Plasmid

    #228532

    Purpose

    A fragment of human Skp1 protein in fusion with FRB domain through a flexible glycine-serine linker.

    Depositor

    Roman Jerala

    Article

    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

    Insert

    myc:Skp1(dC111):gs10:FRB:HA (SKP1 Synthetic, Human)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-HA-GTSE1-S91, 262 ,724A

    Plasmid

    #224737

    Purpose

    For expression of HA-tagged GTSE1 protein with S91, 262 ,724A mutation

    Depositor

    Michele Pagano

    Article

    García-Vázquez et al bioRxiv 2024.06.26.600797

    Insert

    GTSE1 (GTSE1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Serine 91, 262 ,724 to Alanine

    Promoter

    CMV

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-SLC25A46-P2A-Electra1 (pAH9)

    Plasmid

    #227614

    Purpose

    Overexpression of SLC25A46 in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-SLC25A46[∆2-83]-P2A-Electra1 (pAH14)

    Plasmid

    #227619

    Purpose

    Overexpression of SLC25A46 lacking its N-terminal region in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Use

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    deleted aa 2-83

    Promoter

    CMV, T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA

    Plasmid

    #222861

    Purpose

    This plasmid codes for the OgeuIscB nickase

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    OgeuIscB Nickase
    

    Use

    CRISPR

    Tags

    3X HA and Nucleoplasmin NLS and ITR2

    Expression

    Mammalian

    Mutation

    E193A for nickase mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-WT

    Plasmid

    #215898

    Purpose

    Expression of TNRC6A-CIM1 domain

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1224-1528

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-myc-GFP-TNRC6A-SD-CIM2-WT

    Plasmid

    #215899

    Purpose

    Expression of TNRC6A-CIM2 domain

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1529-1709

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(+) EGFP-Rac1(G12C, K96H)

    Plasmid

    #224281

    Purpose

    Cell transfection and expression of Rac1 (G12C, K96H)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G12C, K96H

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+) EGFP-Rap1A(G12C, L96F)

    Plasmid

    #224277

    Purpose

    Cell transfection and expression of Rap1A (G12C, L96F)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rap1A (RAP1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G12C, L96F

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge