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Plasmid#138538PurposeFor making TNKS-2-M1054V RNADepositorInsertTankyrase 2 (TNKS2 Human)
UseIn vitro transcriptionTagsFLAGExpressionMutationChanged methionine 1054 to valinePromoterT7Available sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRMCD28
Plasmid#167556PurposePlasmid pWSK29 encoding E. coli PhoA' for making reporter fusion proteins for topology mappingDepositorTypeEmpty backboneUseTagsPhoA'ExpressionBacterialMutationPromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
C035
Plasmid#174341PurposeTemplate for making Rluc mRNA reporter with pA120.DepositorInsertRenilla luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
C098
Plasmid#174345PurposeTemplate for making Nluc mRNA reporter with pA139.DepositorInsertNanoLuc luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceSept. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
C034
Plasmid#174340PurposeTemplate for making Rluc mRNA reporter with pA98.DepositorInsertRenilla luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
C033
Plasmid#174339PurposeTemplate for making Rluc mRNA reporter with pA63.DepositorInsertRenilla luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
C031
Plasmid#174338PurposeTemplate for making Rluc mRNA reporter with pA30.DepositorInsertRenilla luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceJuly 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
C255
Plasmid#174352PurposeTemplate for making mRNA for overexpressing eGFP (C-FLAG+HA) in frog oocytesDepositorInserteGFP
UseIn vitro transcription templateTagsFLAG and HAExpressionMutationPromoterAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
C071
Plasmid#174343PurposeTemplate for making Nluc mRNA reporter with pA29.DepositorInsertNanoLuc luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceSept. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
C093
Plasmid#174344PurposeTemplate for making Nluc mRNA reporter with pA63.DepositorInsertNanoLuc luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceSept. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
C079
Plasmid#174348PurposeTemplate for making mRNA for overexpressing eGFP in frog oocytesDepositorInserteGFP
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
C022
Plasmid#174337PurposeTemplate for making Rluc mRNA reporter with no pA.DepositorInsertRenilla luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCVD621
Plasmid#66162Purposeto make a ctxB probeDepositorInsertctxB
UseCloning vectorTagsExpressionMutationPromoterUnknownAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRMCD70
Plasmid#167557PurposePlasmid pWSK29 encoding E. coli LacZ' for making reporter fusion proteins for topology mappingDepositorTypeEmpty backboneUseTagsLacZ'ExpressionBacterialMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
CS2-H2B-EGFP
Plasmid#132414PurposeTo make synthetic mRNA encoding a nuclear EGFPDepositorInsertH2B-EGFP (H2BC21 Synthetic)
UseSynthetic mrna productionTagsExpressionMutationPromoterSP6Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL2/4sub
Plasmid#216746PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL2 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL3/4sub
Plasmid#216747PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL3 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL5/4sub
Plasmid#216748PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the variant in which outer loop 4 (OL4) is substituted with OL5 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
UseTags8x HisExpressionBacterialMutationOuter loop 4 (OL4) with the sequence of [TLDGKPVQ…PromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIVT
Plasmid#122139PurposeFor making cRNA appropriate for microinjectionDepositorTypeEmpty backboneUseFor in vitro transcriptionTagsExpressionMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBTG (pBigT-IRES-GFP; DM#268)
Plasmid#15037DepositorTypeEmpty backboneUseCre/Lox; For making transgenic constructsTagsIRES-EGFPExpressionMutationPromoterAvailable sinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
C054
Plasmid#174342PurposeTemplate for making Fluc mRNA reporter with pA124 and mutant mouse Malat1.DepositorInsertFirefly luciferase
UseIn vitro transcription templateTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
HOXC9 (murine) HIS-tag pET
Plasmid#8559DepositorInsertHOXC9 (Hoxc9 Mouse)
UseTagsHis and T7ExpressionBacterialMutationmakes protein by WesternPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Ro1
Plasmid#24504DepositorInsertRASSL Ro1 (OPRK1 Human)
UseTagsHAExpressionMammalianMutationmutations in extracellular loop 2, making it a RA…PromoterAvailable sinceJune 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJL 89
Plasmid#98558PurposeUseful for making infectious clones of RNA virus cDNAs; Has 35S promoter and HDV Ribozyme sequences in T-DNA region of Agrobacterium vector.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterEnh 35SAvailable sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQint
Plasmid#25819DepositorInsertqQint
UseBacterial gene inactivationTagsExpressionMutationplasmid to make targeted insertions in Clostridiu…PromoterAvailable sinceSept. 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMOD_D0000a
Plasmid#127230PurposeEmpty Backbone for golden gate cloning compatible with Cermak., et al Toolkit.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-ActA-IRES-puromycin-pLVx-EF1a
Plasmid#177433PurposeTo express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.DepositorInsertActA tail anchor (actA Listeria monocytogenes)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
HOXA5 (human) HIS-tag pET
Plasmid#8562DepositorInsertHOXA5 (HOXA5 Human)
UseTagsHis and T7ExpressionBacterialMutationSacI site at amino acids 1-2 was used to clone in…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
HOXD9 (human) pET
Plasmid#8570DepositorInsertHOXD9 (HOXD9 Human)
UseTagsT7ExpressionBacterialMutationPCR human cDNA clone; makes protein by in vitro T…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
NK601
Plasmid#109070PurposeParental vector for making S. rosetta expression vectorsDepositorInsertPTSG_03784
UseChoanoflagellate expressionTagsExpressionMutationPromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only