We narrowed to 21,998 results for: his

1. pDZ513 (pHIS MET25pro PP7-PS-yeGFP)

   Plasmid

   #45931

   Depositor

   Daniel Zenklusen

   Article

   Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

   Insert

   PCP-PS
   

   Use

   Tags

   2x-yeGFP

   Expression

   Yeast

   Mutation

   Promoter

   Met25

   Available since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. gWiz_BM40-vGAT-VH-hIgG1-CH1-TS-HIS

   Plasmid

   #218436

   Purpose

   mammalian expression plasmid for the heavy chain fragment of an anti-vGAT Fab domain

   Depositor

   Axel Brunger

   Article

   Gopal et al Curr Res Physiol. 2024 Feb 23;7:100121.

   Insert

   vGAT-Vh-hIgG1-CH1
   

   Use

   Affinity Reagent/ Antibody

   Tags

   BM40 and TwinStrep-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. gWiz_BM40-vGLUT1-VH-hIgG1-CH1-TS-HIS

   Plasmid

   #218438

   Purpose

   mammalian expression plasmid for the heavy chain fragment of an anti-vGLUT1 Fab domain

   Depositor

   Axel Brunger

   Insert

   vGLUT1-Vh-hIgG1-CH2
   

   Use

   Affinity Reagent/ Antibody

   Tags

   BM40 and TwinStrep-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. gWiz_BM40-vMAT2-VH-hIgG1-CH1-TS-HIS

   Plasmid

   #218442

   Purpose

   mammalian expression plasmid for the heavy chain fragment of an anti-vMAT2 Fab domain

   Depositor

   Axel Brunger

   Insert

   vMAT2-Vh-hIgG1-CH4
   

   Use

   Affinity Reagent/ Antibody

   Tags

   BM40 and TwinStrep-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-link-yoLSS-mKate2-SpHis5

   Plasmid

   #44848

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with SpHis5 selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoLSS-mKate2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-16b-10His-TEV-MCES_VACCA_D1_D12

   Plasmid

   #203671

   Purpose

   The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage

   Depositor

   Stephen Cusack

   Article

   Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:

   Insert

   MCES_VACCA_D1_D12
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pnEA-vH_His-TEV-SEPT2-msfGFP

   Plasmid

   #174492

   Purpose

   bacterial expression of human SEPT2 fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Insert

   SEPTIN2 (SEPTIN2 Human)
   

   Use

   Tags

   His6-TEV and msfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. His-CL7-2NLS-SpyCas9(dS)-A22p3star

   Plasmid

   #222970

   Purpose

   Bacterial expression of A22p-fused SpyCas9 construct

   Depositor

   Jennifer Doudna

   Article

   Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

   Insert

   His-CL7-2NLS-SpyCas9(dS)-A22p3
   

   Use

   CRISPR

   Tags

   CL7 and His

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET30-HPF1-His-Sumo-Flag

   Plasmid

   #111577

   Purpose

   Expresses full length HPF1 in E. coli

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   HPF1 (HPF1 Human)
   

   Use

   Tags

   6xHis-Sumo-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEG BacMam C term 3C eGFP His8

    Plasmid

    #160687

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Use

    Tags

    3C eGFP His8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pnEA-vH_His-TEV-SEPT2-msfGFP_SEPT6

    Plasmid

    #174498

    Purpose

    bacterial co-expression of human SEPT2 fused to monomeric superfolder GFP and of human SEPT6

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN2 (SEPTIN2 Human)
    SEPTIN6 (SEPTIN6 Human)
    

    Use

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV-CMV-Flag-PGC1a-6His

    Plasmid

    #67637

    Purpose

    AAV vector expressing PGC1a gene

    Depositor

    Connie Cepko

    Article

    Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

    Insert

    PGC1a (Ppargc1a Mouse)
    

    Use

    AAV

    Tags

    6XHis and Flag tag

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PET-HiFi SpCas9-NLS-6xHis

    Plasmid

    #207376

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HiFi SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R691A

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBAD-HisD-mNG-Ec-Kbp-D9N

    Plasmid

    #178011

    Purpose

    Bacterial expression of green fluorescent potassium indicator mNG-Ec-Kbp-D9N (KRaION1 mutant D9N)

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    mNG-Ec-Kbp-D9N
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araB

    Available since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET21-10XHis-GST-HRV-dL5

    Plasmid

    #73214

    Purpose

    Bacterial expression vector for dL5(E52D) protein with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44

    Insert

    EGFR (EGFR Schistosoma japonicum, Human)
    

    Use

    Tags

    10XHis-GST-HRV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-SARS-CoV-2-S-RBD-8his

    Plasmid

    #145145

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    

    Use

    Tags

    8his tag

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA4/TO 3xFlag-m-PKD1-2xMyc/His

    Plasmid

    #83452

    Purpose

    Full-length murine PC1 for Eukaryotic Expression (982)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    polycystin-1 (Pkd1 Mouse)
    

    Use

    Tags

    2xMyc, 3xFlag, and 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRM055 His-TEV-Cas9 6xNLS 0C

    Plasmid

    #196246

    Purpose

    Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEXP5-NT/6xHis mCherry F30 2xdBroccoli

    Plasmid

    #169233

    Purpose

    Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with 2xd Broccoli RNA aptamer.

    Depositor

    Dora Tang

    Article

    Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

    Insert

    6xHis mCherry F30-2xdBroccoli
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET51b-His-TEV-SNAP-tag

    Plasmid

    #167269

    Purpose

    Overexpression of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAP-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3.1-IL2sp-6His-Asprosin plasmid

    Plasmid

    #190683

    Purpose

    Expresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.

    Depositor

    Atul Chopra

    Article

    Mishra et al STAR Protoc. 2022 Dec 16;3(4):101762. do

    Insert

    IL2-6xHis-Asprosin
    

    Use

    Tags

    IL2 signal peptide, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3.1 His-NLRP1 codon-optimized

    Plasmid

    #166833

    Purpose

    Mammalian codon-optimized NLRP1 with N-terminal and C-terminal HIS tags

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRM053 His-TEV-Cas9 triNLS 0C

    Plasmid

    #196244

    Purpose

    Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. [pSK24] pACYCDuet His-c7orf59|HBXIP

    Plasmid

    #136143

    Purpose

    bacterial expression of c7orf59 and HBXIP

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

    Insert

    c7orf59 and HBXIP (LAMTOR4, LAMTOR5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-26b-Nb127D01-ALFA-His

    Plasmid

    #171568

    Purpose

    Bacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His)

    Depositor

    Hidde Ploegh, Norbert Perrimon

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    Nb127D01-ALFA-His (CXCR2 Human)
    

    Use

    Tags

    ALFA-tag/His-tag and PelB signal peptide

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRM049 His-TEV-Cas9 triNLS 2C

    Plasmid

    #196245

    Purpose

    Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

    Depositor

    Ross Wilson

    Article

    Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA, His6 (N terminal on backbone), NLS, Nucleopla…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. M4754 KanMX::HIS3 Disruptor Converter

    Plasmid

    #51684

    Purpose

    Yeast marker swap plasmid for converting KanMX3 to HIS3

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    HIS3 (HIS3 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEG BacMam N term His8 eGFP 3C

    Plasmid

    #160684

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Use

    Tags

    His8 eGFP 3C

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBAD/myc-his A Rv0712 (FGE)

    Plasmid

    #16132

    Purpose

    Bacterial expression of Mycobacterium tuberculosis FGE

    Depositor

    Carolyn Bertozzi

    Article

    Carrico et al Nat Chem Biol. 2007 Jun . 3(6):321-2.

    Insert

    Formylglycine-generating enzyme
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAG001 His6-TwinStrep-SUMO-AapCas12b

    Plasmid

    #153162

    Purpose

    Bacterial expression plasmid for AapCas12b

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Joung et al medRxiv. 2020 May 8. doi: 10.1101/2020.0

    Insert

    AapCas12b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge