Addgene

We narrowed to 2,417 results for: GEM

Showing: 1921 - 1940 of 2417 results

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pBP-L1U1H09

Plasmid

#73008

Purpose

L1U1H09 terminator

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

L1U1H09 terminator

Use

Synthetic Biology

Tags

Expression

Mutation

CAT gene - C435G (nucleotide) - silent mutagenesi…

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBluescriptKS(-)-FRBx2

Plasmid

#104476

Purpose

Plasmid carrying FRBx2 with C-terminal HA tag.

Depositor

Benjamin Glick

Article

Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

Insert

FRBx2 (MTOR Human)

Use

Phagemid

Tags

HA tag

Expression

Mutation

includes only the FRB domain

Promoter

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-OCAM

Plasmid

#136767

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Ncam2

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Ncam2 (Ncam2 Mouse)

Use

In situ probe

Tags

Expression

Mutation

ISH Probe insert

Promoter

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI5C6A-J23106-geneIII

Plasmid

#80905

Purpose

Phagemid (encodes cI5C6A and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5C6A

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pBP-Bba_K822000

Plasmid

#74094

Purpose

L-Lactate inducible promoter from E. coli MG1655

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Bba_K822000 (L-Lactate promoter - E. coli MG1655)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

NA

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Gucy1b2-probe1

Plasmid

#105454

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. d

Insert

Gucy1b2 (Gucy1b2 Mouse)

Use

To ganerate riboprobe

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI5G6G,P-J23106-geneIII

Plasmid

#80861

Purpose

Phagemid (encodes evolved cI5G6G,P and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5G6G,P

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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DT036A

Plasmid

#159396

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tet promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.tet-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

tet promoter

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pISH-Tshz2

Plasmid

#74361

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tshz2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

teashirt zinc finger family member 2 (Tshz2 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Neurod1

Plasmid

#74396

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Neurod1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Chang et al Gene Expr Patterns. 2016 Jul;21(2):69-80

Insert

neurogenic differentiation 1 (Neurod1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Cck

Plasmid

#105989

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Walz et al J Neurobiol. 2006 Jul . 66(8):835-46.

Insert

cholecystokinin (Cck Mouse)

Use

To generate riboprobe

Tags

Expression

Mutation

Promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pTU1-A-lacZ

Plasmid

#72935

Purpose

Level 1 Destination vector - Position A

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 1 - A site

Use

Synthetic Biology

Tags

Expression

Mutation

Beta-lactamase - G717C (nucleotide) - silent muta…

Promoter

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTC208

Plasmid

#70017

Purpose

TALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based onTomato Yellow Leaf Curl Virus

Depositor

Daniel Voytas

Article

Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:

Insert

Nuclease (TALENs) + Donor + GVR

Use

TALEN

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Neurog1

Plasmid

#74395

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Neurog1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Chang et al Gene Expr Patterns. 2016 Jul;21(2):69-80

Insert

neurogenin 1 (Neurog1 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI5G6T,P-J23106-geneIII

Plasmid

#80862

Purpose

Phagemid (encodes evolved cI5G6T,P and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI5G6T,P

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Pax6

Plasmid

#74350

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pax6. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

paired box 6 (Pax6 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pSB4C5_pBen-BenR

Plasmid

#128127

Purpose

Expressing BenR transcription factor gene under control of benzoate responsive promoter (pBen) in a feedback loop.

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

BenR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pBen

Available since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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Hox A13a

Plasmid

#36463

Depositor

Robert Ho

Article

Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

Insert

hoxA13a (hoxa13a Zebrafish)

Use

Riboprobe synthesis

Tags

Expression

Mutation

contains aa102-290; M107T

Promoter

T7

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
DT043A

Plasmid

#159404

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under T7 bacteriophage promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.T7-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

T7 bacteriophage promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLITMUS-rpoN-cI4A5T6T,P-J23106-geneIII

Plasmid

#80863

Purpose

Phagemid (encodes evolved cI4A5T6T,P and M13 gene III)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

M13 gene III
cI4A5T6T,P

Use

Tags

Expression

Bacterial

Mutation

Promoter

BBa_J23106 and rpoN

Available since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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