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pETM33_USP7_MATH

Plasmid

#178490

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

USP7_MATH (USP7 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGI3EM20C

Plasmid

#135487

Purpose

Agrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hph

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr H2B-tdTomato

Tags

tdTomato

Expression

Bacterial and Yeast

Promoter

SpunH2A/Bpr (divergent)

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pJJiaDEST-APEX2

Plasmid

#149517

Purpose

N-termial APEX2 tag. Gateway destination vector for mammanlian expression

Depositor

Vojo Deretic

Article

Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

Type

Empty backbone

Tags

FLAG-APEX2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_011-TetO-NLS-inactive-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

Plasmid

#228557

Purpose

Expresses inactive RfxCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Article

Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

Insert

dRfxCas13d

Use

Lentiviral

Tags

NLS-HA

Mutation

R239A/H244A/R858A/H863A

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pGI3EM22C

Plasmid

#135488

Purpose

Agrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hph

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr LifeAct-tdTomato

Tags

LifeAct

Expression

Bacterial and Yeast

Promoter

SpunH2A/Bpr (divergent)

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pBUN279

Plasmid

#60674

Purpose

integration of M. smegmatis ald gene in Mycobacterium using mycobacteriophage L5 attB site

Depositor

Raul Barletta

Article

Feng et al J Bacteriol. 2002 Sep;184(18):5001-10.

Insert

ald

Use

E.coli-mycobacterium integration-proficient vector

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pM-ErbB4CTF

Plasmid

#17797

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment only, amino acids 676-1…

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
pOT_7-lenti-EFS-SS1-BBz-2A-puro

Plasmid

#217990

Purpose

Expresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.

Depositor

Neville Sanjana

Article

Xue et al bioRxiv [Preprint]. 2024 Nov 27:2024.11.

Insert

SS1-BBz CAR

Use

Lentiviral

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pOT_1 - lenti-EFS-LTBR-2A-puro

Plasmid

#181970

Purpose

Expresses human LTBR linked to puromycin resistance via P2A for lentiviral delivery.

Depositor

Neville Sanjana

Article

Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

Insert

LTBR (LTBR Human)

Use

Lentiviral

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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VPR_dCas9

Plasmid

#134601

Purpose

Express CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 marker

Depositor

Lasse Ebdrup Pedersen

Article

Karottki et al Biotechnol Bioeng. 2020 Feb;117(2):593-5

Insert

VPR-dCas9-2A-ZsGreen-DR

Tags

VPR and ZsGreen1-DR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pNB813

Plasmid

#60161

Purpose

Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

yEVenus-PEST

Tags

PEST degron

Expression

Yeast

Promoter

SIC1pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pNB774

Plasmid

#60114

Purpose

Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

GrLuc

Use

Unspecified

Tags

ClpXP degron and Nuclear localization signal (NLS…

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pETM33_AP2B1_adaptin

Plasmid

#178467

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

AP2B1_adaptin (AP2B1 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pNB761

Plasmid

#60156

Purpose

Firefly luciferase (Promega) driven by ADE17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

ADE17pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_009-TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-EGFP

Plasmid

#223174

Purpose

Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Article

Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

Insert

RfxCas13d

Use

Lentiviral

Tags

NLS-HA

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pGI3EM09

Plasmid

#135482

Purpose

Agrobacterium T-DNA with SpunHsp70pr driving hph-GFP

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunHsp70pr hph-GFP ADH1t

Tags

GFP

Expression

Bacterial and Yeast

Promoter

SpunHsp70pr

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCK364.306

Plasmid

#192642

Purpose

Expresses dSpRY and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

J306 scRNA

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23119

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET-3c-rhVTN-C

Plasmid

#30224

Depositor

James Thomson

Article

Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

Insert

rhVitronectin with C terminus cleavage (VTN Human)

Expression

Bacterial

Mutation

deleted amino acids: 399-478

Available Since

May 24, 2011

Availability

Academic Institutions and Nonprofits only

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pETM33_SUFU_Sufu

Plasmid

#178488

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

SUFU_Sufu (SUFU Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_PEX14_Pex14

Plasmid

#178484

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

PEX14_Pex14 (PEX14 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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