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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 23,976 results for: nes

Showing: 1921 - 1940 of 23976 results

  1. pDY118A

    Plasmid
    #182958
    Purpose
    pSC101 ori, chl34 resistant. Cas9 induced at 0.4 μg/ml anhydrotetracycline, recombineering proteins induced by a 15 min heat-shock at 42°C, I-Scel (induced by 0.1 mM IPTG) is to cleave the pDonor2.
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Inserts
    Cas9
    Gam, Beta, Exo
    I-scel
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182958-plasmid-map-sequence-id-365360

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  2. pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

    Plasmid
    #128404
    Purpose
    Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Exotoxin A (toxA P. aeruginosa)
    Use
    Tags
    6xHis tag and DsbA signal sequence for periplasmi…
    Expression
    Bacterial
    Mutation
    242DNNNS246 and 384DQNRT388 engineered glycosylat…
    Promoter
    trc
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128404-plasmid-map-sequence-id-261690

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  5. pTrc99S-ssDsbA-TTlight-4xDQNAT

    Plasmid
    #128402
    Purpose
    Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Tetanus toxin light chain domain
    Use
    Tags
    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…
    Expression
    Bacterial
    Mutation
    Inactivating E234A mutation
    Promoter
    trc
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128402-plasmid-map-sequence-id-263357

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  10. pAH-CTX1-rhadCas9-native

    Plasmid
    #129392
    Purpose
    Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    S. pyogenes dCas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine, Histidine 840 to Alanine
    Promoter
    PrhaBAD
    Available since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129392-plasmid-map-sequence-id-266824

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  13. pTE1059

    Plasmid
    #186628
    Purpose
    Expression of S. coelicolor butyrolactone biosynthesis genes in E. coli. Heterologous production of SCBs.
    Depositor
    Eriko Takano
    Article
    Biarnes-Carrera et al ACS Synth Biol. 2018 Apr 20;7(4):1043-10
    Inserts
    scbA
    scbB
    scbC
    Use
    Synthetic Biology
    Tags
    6xHis and 6xHis-thrombin
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic promoter B10, Synthetic promoter B19, a…
    Available since
    Sept. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    186628-plasmid-map-sequence-id-371218

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  16. pMP71-mtGFP-llo-lama4-alg8

    Plasmid
    #174605
    Purpose
    expresses palmytoylated GFP with a C terminal fusion of the LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 and Lama4
    Depositor
    Stanley Riddell
    Article
    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441
    Insert
    palmytoylGFP w/C-term fusion LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 Lama4
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    174605-plasmid-map-sequence-id-344142

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  18. BII-gR-PtW-eSpCas9

    Plasmid
    #133357
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression
    Depositor
    Blair Madison
    Article
    Fischer et al Am J Physiol Gastrointest Liver Physiol.
    Insert
    eSpCas9
    Use
    Tags
    HA-tagged eSpCas9
    Expression
    Mammalian
    Mutation
    eSpCas9 mutations
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133357-plasmid-map-sequence-id-267549

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Showing: 1921 - 1940 of 23976 results