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2,384 results

Showing: 1941 - 1960 of 2384 results

  1. pCas3-APOBEC1

    Plasmid
    #229536
    Purpose
    pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
    Depositor
    Kevin Verstrepen
    Publication
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Inserts
    rAPOBEC1
    Cas3
    Uracil glycosylase inhibitor
    Tags
    XTEN
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae and codon optimi…
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    229536-plasmid-map-sequence-id-458911

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  2. MT2

    Plasmid
    #223330
    Purpose
    Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Publication
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    3HA-TurboID-EGFP-OMP25 (Synj2bp Rat)
    Tags
    3HA-TurboID-EGFP
    Expression
    Plant
    Promoter
    CaMV35S
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    223330-plasmid-map-sequence-id-455593

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  3. pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

    Plasmid
    #202555
    Purpose
    Synaptotagmin-1 sgRNA2 with TagBFP2
    Depositor
    Frederic Meunier
    Publication
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202555-plasmid-map-sequence-id-404691

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  4. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

    Plasmid
    #202556
    Purpose
    Synaptotagmin-1 sgRNA3 with TagBFP2
    Depositor
    Frederic Meunier
    Publication
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202556-plasmid-map-sequence-id-404689

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  5. MT3

    Plasmid
    #223331
    Purpose
    Mapping of cytosol-facing mitochondrion outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cells
    Depositor
    Xiaohong Zhu
    Publication
    Bao et al Plant J. 2024 Apr;118(1):7-23. doi: 10.1
    Insert
    3HA-EGFP-miniTurboID-OMP25 (Synj2bp Rat)
    Tags
    3HA-EGFP-miniTurboID
    Expression
    Plant
    Promoter
    CaMV35S
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    223331-plasmid-map-sequence-id-455811

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  15. icLCT

    Plasmid
    #223179
    Purpose
    Native rat lactase gene (truncated) as an autologous marker (X-Gal, X-Fuc)
    Depositor
    Andreas Stuermer
    Insert
    cytosolic intestinal lactase protein fragment (Lct Rat)
    Expression
    Mammalian
    Available since
    Aug. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    223179-plasmid-map-sequence-id-443108

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  16. pFastBac HTB His-TEV-rDPP9(S729A)-FLAG

    Plasmid
    #217329
    Purpose
    Purification of catalytically-dead rat DPP9 fusion with C-terminal FLAG tagfrom insect cells
    Depositor
    Yifan Wang
    Insert
    DPP9
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    S729A
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217329-plasmid-map-sequence-id-433435

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  17. pFastBac HTB His-TEV-rDPP9-FLAG

    Plasmid
    #217328
    Purpose
    Purification of rat DPP9 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    DPP9
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217328-plasmid-map-sequence-id-433437

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  18. pFastBac HTB His-TEV-rDPP9(S729A)

    Plasmid
    #217327
    Purpose
    Purification of catalytically-dead rat DPP9 from insect cells
    Depositor
    Yifan Wang
    Insert
    DPP9
    Tags
    His-TEV
    Expression
    Insect
    Mutation
    S729A
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217327-plasmid-map-sequence-id-433438

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  19. pFastBac HTB His-TEV-rDPP9

    Plasmid
    #217326
    Purpose
    Purification of rat DPP9 from insect cells
    Depositor
    Yifan Wang
    Insert
    DPP9
    Tags
    His-TEV
    Expression
    Insect
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217326-plasmid-map-sequence-id-433440

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  20. pFastBac HTB His-TEV-BN-rNLRP1(N61K)-FLAG

    Plasmid
    #217325
    Purpose
    Purification of Brown Norway rat NLRP1 (N61K mutation) fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    NLRP1
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    N61K
    Promoter
    polyhedrin
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217325-plasmid-map-sequence-id-438441

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Showing: 1941 - 1960 of 2384 results