2,226 results

1. pEGFP-C1-mScarlet-AMPKα2

   Plasmid

   #192451

   Purpose

   mScarlet-tagged alpha2 isoform of AMPK catalytic subunit.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   mScarlet-AMPKalpha2 (Prkaa2 Rat)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV_pMBP-dnVamp3-P2AT2A-EGFP-caax

   Plasmid

   #190154

   Purpose

   Expresses dominant-negative Vamp3 (rat Vamp3 AA 1-81) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

   Depositor

   Brad Zuchero

   Article

   Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

   Insert

   Vamp3 (Vamp2 Rat)
   

   Use

   AAV

   Tags

   P2AT2A-EGFP-caax

   Expression

   Mammalian

   Mutation

   Truncation that includes only rat Vamp3 AA #1-81

   Promoter

   MBP

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. RAT +D122H

   Plasmid

   #187426

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al bioRxiv 2021.11.29.470343

   Inserts

   ATP1A1 (Atp1a1 Rat)
   ATP1B1 (Atp1b1 Rat)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   changed aspartic acid at 129 for histidine

   Promoter

   PH and p10

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. RAT +R111E

   Plasmid

   #187425

   Purpose

   Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al bioRxiv 2021.11.29.470343

   Inserts

   ATP1A1 (Atp1a1 Rat)
   ATP1B1 (Atp1b1 Rat)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   changed arginine at 118 to glutamic acid

   Promoter

   PH and p10

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. VAMP8

   Plasmid

   #186689

   Purpose

   Expresses VAMP8 in E. Coli

   Depositor

   Axel Brunger

   Article

   Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

   Insert

   VAMP8
   

   Use

   Tags

   3xFLAG, GST, TEV, and Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-Myo1b motor

   Plasmid

   #187364

   Purpose

   Expresses rat myosin 1b isoform b motor domain labelled with EGFP

   Depositor

   Evelyne Coudrier

   Article

   Prosperi et al J Cell Biol. 2015 Jul 20;210(2):347-61.

   Insert

   myosin 1b isoform b motor domain (Myo1b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Stx3

   Plasmid

   #186685

   Purpose

   Expresses Syntaxin 3 in E. Coli

   Depositor

   Axel Brunger

   Article

   Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

   Insert

   Syntaxin 3
   

   Use

   Tags

   6 his tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-Myo1b-Tail

   Plasmid

   #187365

   Purpose

   Expresses rat myosin 1b isoform b Tail domain labelled with EGFP

   Depositor

   Evelyne Coudrier

   Article

   Prosperi et al J Cell Biol. 2015 Jul 20;210(2):347-61.

   Insert

   myosin 1b isoform b Tail domain (Myo1b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EGFP-Myo1-IQ-Tail

   Plasmid

   #187363

   Purpose

   Expresses rat myosin 1b isoform b IQTail domain labelled with EGFP

   Depositor

   Evelyne Coudrier

   Article

   Raposo et al Mol Biol Cell. 1999 May . 10(5):1477-94.

   Insert

   myosin 1b isoform b IQTail domain (Myo1b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-TetO(3G)-GCaMP6f-WPRE

    Plasmid

    #186205

    Purpose

    Express GCaMP6f under TetO(3G)

    Depositor

    Naoshige Uchida

    Article

    Amo et al Nat Neurosci. 2022 Aug;25(8):1082-1092.

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    6xHis (N terminal on insert), T7 epitope, and Xpr…

    Expression

    Mutation

    Promoter

    TetO(3G)

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-mCherry-SynaptoZip

    Plasmid

    #177318

    Purpose

    AAV expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by human Synapsin promoter

    Depositor

    Antonio Malgaroli

    Article

    Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

    Insert

    mCherry-SynaptoZip (Vamp2 Rat)
    

    Use

    AAV

    Tags

    Myc and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFUGW-shRIIα

    Plasmid

    #183454

    Purpose

    sgRNA targeting rat PKA-RIIα subunits

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA targeting rat PKA-RIIα (Prkar2a Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFUGW-scrambled

    Plasmid

    #183455

    Purpose

    This control vector contains a scrambled version of the targeting sequence used in the pFUGW-shRIIα construct

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    scrambled sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFUGW-shRIIα-RIIαS97A-IRES-EGFP

    Plasmid

    #183457

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUGW-shRIIα-RIIαS97E-IRES-EGFP

    Plasmid

    #183458

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pORANGE_Tubb3 GFP(Y39TAG) KI

    Plasmid

    #182678

    Purpose

    Expression of spCas9, gRNA targeting the end of Tubb3 gene and donor GFP(Y39TAG). Can be used for amber codon suppression and click chemistry labeling of endogenous beta-3 tubulin in mammalian cells.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    Tubb3-targeting gRNA and GFP(Y39TAG) donor sequence (Tubb3, Tubb3 Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken beta-actin promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. iLID-mRuby3-iTrkA

    Plasmid

    #136510

    Purpose

    Mammalian expression of iLID fused to mRuby3 and intracellular signaling domain of TrkA (aa 450-799)

    Depositor

    Bianxiao Cui

    Article

    Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

    Insert

    iLID mRuby3 iTrkA (Ntrk1 Rat, Avena sativa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLZRS-mVenus-TrkC

    Plasmid

    #182948

    Purpose

    To express the rat TrkC fused in the N-terminus with mVenus

    Depositor

    Pantelis Tsoulfas

    Insert

    mVenus-TrkC (Ntrk3 Rat, Synthetic)
    

    Use

    Retroviral and Synthetic Biology

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    MMLV

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLZRS-TrkC-mCherry

    Plasmid

    #182949

    Purpose

    To express the rat TrkC fused in the C-terminus with mCherry

    Depositor

    Pantelis Tsoulfas

    Insert

    TRk-C-mCherry (Ntrk3 Rat, Synthetic)
    

    Use

    Retroviral and Synthetic Biology

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    MMLV

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBBK88 pHomL-TDH3p-Neon(gfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)

    Plasmid

    #179072

    Purpose

    Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp)-Link-2xPH(PLCd), an overexpressed (bright) marker for the plasma membrane

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    TDH3p-Neon(gfp)-Link-2xPH(PLCd)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge