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Plasmid#110323PurposeTRCN0000378630 (Target GATAAGTACCGTTGAGTTTG), silence human HRASLS gene and express monomeric Kusabira-Orange2DepositorInsertHRASLS (PLAAT1 Homo sapiens, Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNA fo…Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAP1893-1
Plasmid#105248Purposehuman GFP11 with tagRFP 33bp downstream in Lamin A/C (recoded)DepositorInsertInsertion of GFP11 at cut tagRFP 33bp downstream (recoded *) in Lamin A/C (LMNA Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
10xHis-ataxin-3 *SIM-HA
Plasmid#89983PurposeExpresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motifDepositorInsertataxin-3 (ATXN3 Human)
UseTags10xHis and HAExpressionMammalianMutationmutated aa 162IFVV to 162AFAAPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-ataxin-3 *SIM
Plasmid#89979PurposeExpresses GFP-tagged ataxin-3 with a mutation in the SUMO-interacting motifDepositorInsertataxin-3 (ATXN3 Human)
UseTagsGFPExpressionMammalianMutationmutated 162IFVV to 162AFAAPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-ataxin-3 *VBM
Plasmid#89976PurposeExpresses GFP-tagged ataxin-3 with a mutation in the VCP-binding motifDepositorInsertataxin-3 (ATXN3 Human)
UseTagsGFPExpressionMammalianMutationmutated 282RKRR to 282HNHHPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTmiRc2_3xflag_CHEK2
Plasmid#136526PurposeUsed as a donor vector containing N-term 3xFLAG to clone into pSLIKDepositorInsertCHEK2 (CHEK2 Human)
UseEntry vector for gateway cloningTags3x FLAGExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 hygro
Plasmid#104995PurposeThis lentiviral construct delivers hSpCas9 and hygromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-zSpG
Plasmid#179316PurposeIn vitro transcription of SpG-Cas9 mRNA zebrafish codon-optimized from T3 promoterDepositorInsertZebrafish codon-optimized Cas9 variant SpG
UseCRISPR; In vitro transcription by t3 promoterTagsSV40-NLSExpressionMutationSpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337RPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-zSpRY
Plasmid#179317PurposeIn vitro transcription of SpRY-Cas9 mRNA zebrafish codon-optimized from T3 promoterDepositorInsertZebrafish codon-optimized Cas9 variant SpRY
UseCRISPR; In vitro transcription by t3 promoterTagsSV40-NLSExpressionMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…PromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA puro
Plasmid#104990PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA hygro
Plasmid#104991PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA blast
Plasmid#104993PurposeThis 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD deltahexa
Plasmid#169714PurposeBacterial Expression of human hnRNPA1-A Low complexity domain including amino acids 186-320, hexapeptide including aa 259-264 deletedDepositorInserthnRNPA1 (HNRNPA1 Human)
UseTags6xHisExpressionBacterialMutationhnRNPA1-A Low complexity domain including amino a…PromoterT7Available sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 puro
Plasmid#104994PurposeThis 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWZL-Neo-Myr-Flag-DEST
Plasmid#15300Purposea Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORFDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 neo
Plasmid#104996PurposeThis lentiviral construct delivers hSpCas9 and G418 resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRM210 LGP (Luciferase GFP Puro)
Plasmid#174722PurposeExpresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance geneDepositorInsertsFirefly Luciferase
eGFP
pac
UseLentiviral and LuciferaseTagsE2A linker and F2A linkerExpressionMammalianMutationPromoterMND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-puro /BRAF-V600E
Plasmid#131724PurposeMammalian Expression of Flag tagged BRAFDepositorInsertBRAF (BRAF Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationV600EPromoterCMVAvailable sinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGTag-NLS-eGFP-SV40
Plasmid#117811PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInserteGFP
UseSynthetic BiologyTagsNLSExpressionMutationPromoterAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLIK Zeo p53DD
Plasmid#136534PurposeLentiviral expression vector for an inducible p53DDDepositorInsertp53DD (Trp53 Mouse)
UseLentiviral; Destinatioin vector for gateway cloni…TagsExpressionMammalianMutationThis construct was created from the pBabe p53DD (…PromoterAvailable sinceFeb. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC1-V16-Luc
Plasmid#122034PurposeAAV vector, pTet bidi (bidirectional tetracycline-responsive promoter) driving Tet-dependent rtTA and Luciferase expressionDepositorInsertLuciferase
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311/NLS-Cas13d-P2A-Blast
Plasmid#212196PurposeCasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40DepositorInsertCasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
UseCRISPR and LentiviralTagsHA, NLS, and blasticidin S deaminaseExpressionMammalianMutationPromoterEF-1α promoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2 LRP6-APEX2-Ires-eGFP-PAC
Plasmid#180142Purposeused to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periodsDepositorInsertHuman LRP6 (LRP6 Human)
UseTagsExpressionMammalianMutationPromoterSP6Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-puro/RAF1
Plasmid#131725PurposeMammalian Expression of RAF1DepositorInsertRAF1 (RAF1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-wtYAP
Plasmid#84009Purposeexpresses wtYAP in mammalian cells under the control of a doxycycline-inducible promoterDepositorInsertYAP1 (siRNA insensitive) (YAP1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterTRE-CMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX304_zeo_mmIrf1
Plasmid#160098PurposeExpresses murine Irf1 in mammalian cellsDepositorInsertIrf1 (Irf1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO.neo_shGFP
Plasmid#110470PurposeControl, silence GFP gene, doxycycline inducible, neomycin selectionDepositorInsertGFP
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1/TO (RNA PolIII)Available sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti-SpCas9 blast
Plasmid#104997PurposeThis lentiviral construct delivers hSpCas9 and blasticidin S resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-puro/RAF1-S259A
Plasmid#131727PurposeMammalian Expression of RAF1DepositorInsertRAF1 (RAF1 Human)
UseLentiviralTagsExpressionMammalianMutationS259APromoterCMVAvailable sinceMay 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF CAG BFP2 IRES neo
Plasmid#108175PurposeExpresses a blue fluorescent protein (BFP2) in mammalian cells along with conferring resistance to G418DepositorInsertBlue fluorescent protein (BFP2)
UseLentiviral; Fluorescent proteinTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only