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Showing: 1941 - 1960 of 19449 results

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pCMV-Tag3B-CCre

Plasmid

#106369

Purpose

C-terminal part of the split-Cre System

Depositor

Johannes Hirrlinger

Publication

Hirrlinger et al PLoS One. 2009;4(1):e4286. doi: 10.1371/

Insert

CCre

Use

Cre/Lox

Tags

c-myc

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pACYC-Skp-TF

Plasmid

#83921

Purpose

Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Publication

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Trigger Factor

Expression

Bacterial

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-C1-CAMKIIalpha-T286A

Plasmid

#21220

Depositor

Tobias Meyer

Publication

Shen et al Science. 1999 Apr 2. 284(5411):162-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CAMKIIalpha-T286A (Camk2a Rat)

Tags

GFP

Expression

Mammalian

Mutation

T286A (Lab plasmid KS 0003).

Available since

July 29, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
tet_pLKO.1_puro_shNRF2 #2

Plasmid

#136585

Purpose

Expresses an inducible short hairpin targeting human NRF2 sequence

Depositor

Kevin Janes

Publication

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

shNRF2v2 (NFE2L2 Human)

Use

Lentiviral

Expression

Mammalian

Available since

June 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCMV-dCas13-inactive M3nls

Plasmid

#157854

Purpose

Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

Depositor

David Liu

Publication

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A; METTL3 D395A

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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STAT3(Y705F)-TAL-Luc

Plasmid

#46933

Purpose

Luciferase Reporter for mutant STAT3

Depositor

Afshin Dowlati

Publication

Dabir et al Mol Cancer Res. 2009 Nov;7(11):1854-60.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

STAT3(Y705F) (STAT3 Human)

Use

Luciferase

Tags

FLAG

Mutation

Tyrosine 705 to Phenylalanine

Promoter

TA Luciferase

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pAC91-pmax-dCas9VP64

Plasmid

#48223

Purpose

dCas9VP64 on pmax expression vector

Depositor

Rudolf Jaenisch

Publication

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9(D10A;H840A) fusion with VP64 activation domain

Use

CRISPR

Tags

HA Tag and VP64

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pyEvolvR (enCas9-PolI5M)

Plasmid

#158073

Purpose

Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber

Publication

Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

Insert

enCas9-PolI5M

Expression

Bacterial and Yeast

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pRSF-MCM3/5

Plasmid

#116950

Purpose

Expresses human MCM3 and human MCM5

Depositor

James Chong

Publication

Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

Inserts

Human MCM5 (MCM5 Human)
human MCM3 (MCM3 Human)

Tags

His6

Expression

Bacterial

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-GECO2G

Plasmid

#159605

Purpose

AAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2G

Depositor

Robert Campbell

Publication

Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

Has service

AAV1, AAV2, and AAV9

Insert

NIR-GECO2G

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMU-MTUBr

Plasmid

#61196

Purpose

Fluorescent reporter for microtubules mCherry-MAP4-MBD expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MAP-MBD

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA4-Beclin1 1-150

Plasmid

#24389

Depositor

Qing Zhong

Publication

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Beclin1 (BECN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains amino acids 1-150.

Available since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pLH-sgRNA-Sirius-4X(MS2-PP7)

Plasmid

#121941

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Publication

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-4X(MS2-PP7)

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

human U6 promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSR660

Plasmid

#65216

Purpose

Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells

Depositor

Robert Kotin

Publication

Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

Inserts

modified AAV-2 rep78
modified AAV-8 cap

Use

AAV

Expression

Insect

Promoter

P10 and polyhedrin

Available since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGEX-6P-1 SARS-CoV 3CLpro

Plasmid

#61692

Purpose

inducible expression of SARS 3CLpro protease

Depositor

Michael Johnson

Publication

Lee et al Bioorg Med Chem. 2014 Jan 1;22(1):167-77

Insert

SARS 3CLpro protease

Tags

GST and HIS

Expression

Bacterial

Promoter

tac

Available since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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CF-PIPKi

Plasmid

#20158

Depositor

Tobias Meyer

Publication

Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CFP-FKBP-PIPK (D253A) (Pip5k1c Mouse)

Expression

Mammalian

Mutation

D253A mutation in PIPK. C-terminus (amino acids …

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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pF149 pSOD1G85RAcGFP1

Plasmid

#26410

Depositor

Elizabeth Fisher

Publication

Stevens et al PLoS One. 2010 . 5(3):e9541.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

G85R

Available since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles
pDEST-V2-ORF

Plasmid

#73636

Purpose

Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 2 (V2).

Depositor

Darren Saunders

Publication

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 2 (V2)

Expression

Mammalian

Promoter

CMV

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-hSyn-NES-NIR-GECO1

Plasmid

#113683

Purpose

Express NIR-GECO1 preferentially in neurons

Depositor

Robert Campbell

Publication

Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

Insert

NES-NIR-GECO1

Use

AAV

Promoter

hSyn

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Nanog

Plasmid

#40800

Depositor

Rudolf Jaenisch

Publication

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Nanog (Nanog Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles