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pRSF-MCM3/5

Plasmid

#116950

Purpose

Expresses human MCM3 and human MCM5

Depositor

James Chong

Publication

Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

Inserts

Human MCM5 (MCM5 Human)
human MCM3 (MCM3 Human)

Tags

His6

Expression

Bacterial

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-GECO2G

Plasmid

#159605

Purpose

AAV production plasmid encoding for near-infrared calcium indicator NIR-GECO2G

Depositor

Robert Campbell

Publication

Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

Has service

AAV1, AAV2, and AAV9

Insert

NIR-GECO2G

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p21 promoter A

Plasmid

#16462

Depositor

Bert Vogelstein

Publication

el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

Insert

p21 promoter (CDKN1A Human)

Expression

Mammalian

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV-dCas13-inactive M3nls

Plasmid

#157854

Purpose

Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

Depositor

David Liu

Publication

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)

Expression

Mammalian

Mutation

PspCas13b Δ984-1090 H133A; METTL3 D395A

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLH-sgRNA-Sirius-4X(MS2-PP7)

Plasmid

#121941

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Publication

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-4X(MS2-PP7)

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

human U6 promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRK5-HA-UFM1

Plasmid

#134639

Purpose

expression of HA-tagged human UFM1 wildtype

Depositor

Yihong Ye

Publication

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFM1 (UFM1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSR660

Plasmid

#65216

Purpose

Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells

Depositor

Robert Kotin

Publication

Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

Inserts

modified AAV-2 rep78
modified AAV-8 cap

Use

AAV

Expression

Insect

Promoter

P10 and polyhedrin

Available since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGEX-6P-1 SARS-CoV 3CLpro

Plasmid

#61692

Purpose

inducible expression of SARS 3CLpro protease

Depositor

Michael Johnson

Publication

Lee et al Bioorg Med Chem. 2014 Jan 1;22(1):167-77

Insert

SARS 3CLpro protease

Tags

GST and HIS

Expression

Bacterial

Promoter

tac

Available since

April 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pcDNA3/RGS-UBC12

Plasmid

#20080

Depositor

Edward Yeh

Publication

Gong et al J Biol Chem. 1999 Apr 23;274(17):12036-4

Insert

UBC12 (UBE2M Human)

Tags

6xHis

Expression

Mammalian

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Strep-KIFC1*-mCh

Plasmid

#120169

Purpose

Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)

Depositor

Juan Bonifacino

Publication

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member C1 (KIFC1 Human)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIFC1: 125-673 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDEST-V1-ORF

Plasmid

#73635

Purpose

Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 1 (V1).

Depositor

Darren Saunders

Publication

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 1 (V1)

Expression

Mammalian

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLPC-MYC-hTRF1

Plasmid

#64164

Purpose

Retroviral vector expressing human TRF1 with N-terminal MYC tag

Depositor

Titia de Lange

Publication

Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mTFP1-Lifeact-7

Plasmid

#54749

Purpose

Localization: Actin, Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Publication

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Lifeact-mTFP1

Expression

Mammalian

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pAAV-hSyn-NES-NIR-GECO1

Plasmid

#113683

Purpose

Express NIR-GECO1 preferentially in neurons

Depositor

Robert Campbell

Publication

Qian et al Nat Methods. 2019 Feb;16(2):171-174. doi

Insert

NES-NIR-GECO1

Use

AAV

Promoter

hSyn

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Nanog

Plasmid

#40800

Depositor

Rudolf Jaenisch

Publication

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Nanog (Nanog Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

Plasmid

#183409

Purpose

piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

Depositor

Azim Surani

Publication

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1

Use

CRISPR and Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE3G

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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5HT6-G-GECO1.0

Plasmid

#47499

Purpose

Fluorescent reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Publication

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

5-hydroxytriptamine receptor isoform 6 (Htr6 Mouse)

Tags

G-GECO1.0

Expression

Mammalian

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA6-N-3XFLAG-Ctnnb1

Plasmid

#123586

Depositor

Bruce Beutler

Publication

Choi et al Science. 2019 May 10;364(6440). pii: 364

Insert

catenin (cadherin associated protein), beta 1 (Ctnnb1 Mouse)

Tags

FLAG

Expression

Mammalian

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pScalps_Puro_mCebpa

Plasmid

#79551

Purpose

Expression of mouse Cebpa

Depositor

Silvia Monticelli

Publication

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Cebp alpha (Cebpa Mouse)

Use

Lentiviral

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP-hRhoD

Plasmid

#23235

Depositor

Channing Der

Publication

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

RhoD (RHOD Human)

Tags

EGFP

Expression

Mammalian

Available since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article