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Showing: 4901 - 4950 of 8645 results

  1. pHS201-ophi

    Plasmid
    #227349
    Purpose
    Heterologous production of ophiobolin C in Aspergillus oryzae
    Depositor
    Jun-ichi Maruyama
    Insert
    The hoomlogous regions of the HS201 locus and expression cassettes of the AcOS, oblDBm, and oblBBm genes
    Use
    Synthetic Biology
    Available Since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    227349-plasmid-map-sequence-id-459986

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  2. pwAB-erg8erg19idi1erg20

    Plasmid
    #227345
    Purpose
    Overexpression of the erg8, erg19, idi1, and erg20 genes in Aspergillus oryzae
    Depositor
    Jun-ichi Maruyama
    Insert
    Expression cassettes of the erg8, erg19, idi1, and erg20 genes
    Use
    Synthetic Biology
    Available Since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    227345-plasmid-map-sequence-id-458568

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  3. ppG-ple12

    Plasmid
    #227339
    Purpose
    Heterologous production of pleuromutilin in Aspergillus oryzae
    Depositor
    Jun-ichi Maruyama
    Insert
    The upstream/downstream regions of the pyrG gene and expression cassettes of the ple1 and ple2 genes
    Use
    Synthetic Biology
    Available Since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    227339-plasmid-map-sequence-id-458622

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  4. pLSU1/t35s[TMV]:MCS:NosT

    Plasmid
    #212158
    Purpose
    This binary vector has an empty backbone where you can insert a gene into a cassette with a strong promoter (truncated 35sCAMV) and UTR (Tomato Mosaic Virus)
    Depositor
    Wayne Curtis
    Type
    Empty backbone
    Expression
    Plant
    Available Since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212158-plasmid-map-sequence-id-422545

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  5. pSW007

    Plasmid
    #201326
    Purpose
    pDefence7:Eluc (defence-induced promoter with green-shifted luciferase expression cassette)
    Depositor
    Benjamin Schwessinger
    Insert
    pDefence7:Eluc
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201326-plasmid-map-sequence-id-410568

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  6. pSW003

    Plasmid
    #201322
    Purpose
    pDefence3:Eluc (defence-induced promoter with green-shifted luciferase expression cassette)
    Depositor
    Benjamin Schwessinger
    Insert
    pDefence3:Eluc
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201322-plasmid-map-sequence-id-410566

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  7. pSW002

    Plasmid
    #201321
    Purpose
    pDefence2:Eluc (defence-induced promoter with green-shifted luciferase expression cassette)
    Depositor
    Benjamin Schwessinger
    Insert
    pDefence2:Eluc
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201321-plasmid-map-sequence-id-410564

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  8. pSW001

    Plasmid
    #201320
    Purpose
    pDefence1:Eluc (defence-induced promoter with green-shifted luciferase expression cassette)
    Depositor
    Benjamin Schwessinger
    Insert
    pDefence1:Eluc
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201320-plasmid-map-sequence-id-410559

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  9. pBD002

    Plasmid
    #201331
    Purpose
    pDefence15:Eluc (defence-induced promoter with green-shifted luciferase expression cassette)
    Depositor
    Benjamin Schwessinger
    Insert
    pDefence15:Eluc
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201331-plasmid-map-sequence-id-410554

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  10. pSW010

    Plasmid
    #201327
    Purpose
    pDefence10:Eluc (defence-induced promoter with green-shifted luciferase expression cassette)
    Depositor
    Benjamin Schwessinger
    Insert
    pDefence10:Eluc
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201327-plasmid-map-sequence-id-410538

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  11. pSW004

    Plasmid
    #201323
    Purpose
    pDefence4:Eluc (defence-induced promoter with green-shifted luciferase expression cassette)
    Depositor
    Benjamin Schwessinger
    Insert
    pDefence4:Eluc
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201323-plasmid-map-sequence-id-410534

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  12. pSW012

    Plasmid
    #201329
    Purpose
    pDefence12:Eluc (defence-induced promoter with green-shifted luciferase expression cassette)
    Depositor
    Benjamin Schwessinger
    Insert
    pDefence12:Eluc
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201329-plasmid-map-sequence-id-410532

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  13. pSW006

    Plasmid
    #201325
    Purpose
    pDefence6:Eluc (defence-induced promoter with green-shifted luciferase expression cassette)
    Depositor
    Benjamin Schwessinger
    Insert
    pDefence6:Eluc
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    201325-plasmid-map-sequence-id-410530

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  14. pSW005

    Plasmid
    #201324
    Purpose
    pDefence5:Eluc (defence-induced promoter with green-shifted luciferase expression cassette)
    Depositor
    Benjamin Schwessinger
    Insert
    pDefence5:Eluc
    Expression
    Plant
    Mutation
    removal of BsaI and BpiI sites
    Available Since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    201324-plasmid-map-sequence-id-410074

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  15. pUC119-MCS

    Plasmid
    #58807
    Purpose
    contains multiple cloning sites (MCSs) for cloning and transient expression of single or multiple guide RNA (gRNA) expression cassettes.
    Depositor
    Jen Sheen
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    58807-plasmid-map-sequence-id-91105

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  16. PB-TET-PH-lox66FRT

    Plasmid
    #139561
    Purpose
    piggyBac expression vector with TET-ON system and puromycin selection
    Depositor
    Minoru Ko
    Insert
    PGK-puroR-pA cassette, inserted in PB-TET vector
    Use
    Piggybac
    Expression
    Mammalian
    Available Since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139561-plasmid-map-sequence-id-274608

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  17. PB-TET-ZH-lox66FRT

    Plasmid
    #139563
    Purpose
    piggyBac expression vector with TET-ON system and Zeocin selection
    Depositor
    Minoru Ko
    Insert
    PGK-ZeoR-pA cassette, inserted in PB-TET vector
    Use
    Piggybac
    Expression
    Mammalian
    Available Since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139563-plasmid-map-sequence-id-274610

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  18. PB-TET-BH-lox66FRT

    Plasmid
    #139562
    Purpose
    piggyBac expression vector with TET-ON system and Blasticidin selection
    Depositor
    Minoru Ko
    Insert
    PGK-BSD-pA cassette, inserted in PB-TET vector
    Use
    Piggybac
    Expression
    Mammalian
    Available Since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139562-plasmid-map-sequence-id-274605

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  19. pTU2-a_Sv1KA_amilCP

    Plasmid
    #204054
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var1 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204054-plasmid-map-sequence-id-418176

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  20. pTU2-a_Sv2KA_amilCP

    Plasmid
    #204053
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var2 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204053-plasmid-map-sequence-id-418175

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  21. pAW8-aid-Turg1:kanMX6

    Plasmid
    #169354
    Purpose
    C-terminal aid degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Expression
    Yeast
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169354-plasmid-map-sequence-id-333202

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  22. pAW8-aid-V5-Turg1:kanMX6

    Plasmid
    #169357
    Purpose
    C-terminal aid-V5 degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Tags
    1XV5
    Expression
    Yeast
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169357-plasmid-map-sequence-id-333200

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  23. pREP4xN_ccdB

    Plasmid
    #79022
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79022-plasmid-map-sequence-id-147779

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  24. pREP3xN_ccdB

    Plasmid
    #79021
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, LEU2 selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79021-plasmid-map-sequence-id-151965

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  25. pREP82xN_ccdB

    Plasmid
    #79026
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79026-plasmid-map-sequence-id-147783

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  26. pREP81xN_ccdB

    Plasmid
    #79025
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79025-plasmid-map-sequence-id-151959

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  27. pTU2-a_Sv5KA_amilCP

    Plasmid
    #204050
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var5 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    204050-plasmid-map-sequence-id-437162

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  28. pTU2-a_Sv3KA_amilCP

    Plasmid
    #204052
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var3 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204052-plasmid-map-sequence-id-418172

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  29. pTU2-a_Sv6KA_amilCP

    Plasmid
    #204049
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var6 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204049-plasmid-map-sequence-id-418169

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  30. pTU2-a_Sv7KA_amilCP

    Plasmid
    #204048
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var7 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204048-plasmid-map-sequence-id-418164

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  31. pTU2-a_Sv4KA_amilCP

    Plasmid
    #204051
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var4 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204051-plasmid-map-sequence-id-413880

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  32. pANY4

    Plasmid
    #164897
    Purpose
    Two valuable constructs for either IPTG-induced or temperature-induced expression can be simultaneously obtained in a single cloning process by utilizing plasmid pANY4.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Tags
    His-tag
    Expression
    Bacterial
    Available Since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164897-plasmid-map-sequence-id-327986

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  33. SP65U1-135a-2

    Plasmid
    #105820
    Purpose
    plasmid for the overexpression of miR-135a under the U1 expression cassette inserted in the pSP65 plasmid
    Depositor
    Cecilia Mannironi
    Insert
    MIRNA135A2 (Mir135a-2 Mouse)
    Expression
    Mammalian
    Promoter
    U1
    Available Since
    Jan. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    105820-plasmid-map-sequence-id-202522

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  34. pREP41xN_ccdB

    Plasmid
    #79023
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Aug. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    79023-plasmid-map-sequence-id-151952

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  35. pREP42xN_ccdB

    Plasmid
    #79024
    Purpose
    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    July 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    79024-plasmid-map-sequence-id-147781

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  36. R6K_tac_mCherry_ChInt

    Plasmid
    #187382
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible mCherry
    Depositor
    Roi Avraham
    Insert
    mCherry
    Expression
    Bacterial
    Available Since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    187382-plasmid-map-sequence-id-371124

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  37. R6K_tac_GFPmut3_ChInt

    Plasmid
    #187381
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible GFPmut3
    Depositor
    Roi Avraham
    Insert
    GFPmut3
    Expression
    Bacterial
    Available Since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    187381-plasmid-map-sequence-id-370413

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  38. cEF1a-LSL-GFP

    Plasmid
    #135672
    Purpose
    Introduce Cre-inducible (EF1a promoter followed by lox-stop-lox) GFP cDNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    GFP
    Use
    Mouse Targeting
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135672-plasmid-map-sequence-id-325851

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  39. pAW8-aid-eDSR-Turg1:kanMX6

    Plasmid
    #169362
    Purpose
    C-terminal aid-eDSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Expression
    Yeast
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169362-plasmid-map-sequence-id-333207

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  40. pDest_PANCEd_AraC_S170_LOV_sd2_WT

    Plasmid
    #242151
    Purpose
    Expression Plasmid containing a LacI repressed sd2 WT AraC cassette with a LOV domain insertion at S170
    Depositor
    Dominik Niopek
    Insert
    AraC S170 LOV
    Expression
    Bacterial
    Available Since
    Aug. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    242151-plasmid-map-sequence-id-484656

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  41. pLSU1/t35s[AtPsaK]:MCS+:NosT

    Plasmid
    #212159
    Purpose
    This binary vector has an empty backbone where you can insert a gene into a cassette with a strong promoter (truncated 35sCAMV) and UTR (truncated Arabidopsis thaliana PsaK)
    Depositor
    Wayne Curtis
    Type
    Empty backbone
    Expression
    Plant
    Available Since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212159-plasmid-map-sequence-id-422849

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  42. pAW8-aid-spo5DSR-Turg1:kanMX6

    Plasmid
    #169358
    Purpose
    C-terminal aid-spo5DSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Expression
    Yeast
    Available Since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    169358-plasmid-map-sequence-id-333930

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  43. pANY8

    Plasmid
    #164900
    Purpose
    Containing His-ELP-intein multifunctional tag for protein purification by combining Ni-NTA-based and ELP-based strategy with a specially designed purification protocol.
    Depositor
    Yingfeng An
    Insert
    ccdB cassette
    Tags
    His-ELP-Intein-tag
    Expression
    Bacterial
    Available Since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    164900-plasmid-map-sequence-id-330483

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  44. pJM101: pHps1 RFP on pCM66T

    Plasmid
    #74740
    Purpose
    pCM66T with promoter pHps1 driving RFP expression (maxRFP = BBa_E1010)
    Depositor
    Mary Lidstrom
    Insert
    RFP
    Promoter
    pHps1 (extracted from Methylovorus glucosetrophus…
    Available Since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74740-plasmid-map-sequence-id-146986

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  45. pRSFDuet-galK

    Plasmid
    #193727
    Purpose
    Contains expression cassette for E.coli galK for use as a counterselection marker in strains lacking galK
    Depositor
    Martin Buck
    Insert
    galK (galK E. coli)
    Expression
    Bacterial
    Promoter
    J23113
    Available Since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193727-plasmid-map-sequence-id-381922

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  46. pDual_dsCas9_Blast

    Plasmid
    #214681
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sites
    Depositor
    Kevin Janes
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214681-plasmid-map-sequence-id-428811

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  47. pBHt2G-RFP

    Plasmid
    #107162
    Purpose
    Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)
    Depositor
    Sophien Kamoun
    Type
    Empty backbone
    Use
    Fungal grobacterium transformation plasmid, teste…
    Available Since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    107162-plasmid-map-sequence-id-205976

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  48. pUPRT::Floxed DHFR-TS*

    Plasmid
    #100606
    Purpose
    TgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.
    Depositor
    David Sibley
    Insert
    TgUPRT::Floxed TgDHFR-TS*
    Use
    Toxoplasma expression
    Mutation
    Pyrimethamine resistance
    Promoter
    pTgDHFR-TS
    Available Since
    Sept. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    100606-plasmid-map-sequence-id-192862

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  49. pIVTR(T7A)-CRE-T2A-PuroR

    Plasmid
    #172298
    Purpose
    In vitro transcription of CRE-T2A-PuroR synthetic construct for the excision of floxed cassettes
    Depositor
    Patrik Verstreken
    Insert
    CRE-T2A-PuroR
    Use
    Cre/Lox
    Promoter
    T7 promoter A-initiating
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172298-plasmid-map-sequence-id-340323

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  50. pAW8-aid-3HA-Turg1:kanMX6

    Plasmid
    #169355
    Purpose
    C-terminal aid-3HA degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Tags
    3XHA
    Expression
    Yeast
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169355-plasmid-map-sequence-id-333206

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