We narrowed to 2,125 results for: PAM

1. pMCh-658

   Plasmid

   #92257

   Purpose

   Plasmid for expression of NHA-tagged human TNRC6C CED in mammalian cells with mutated PAM2 motif

   Depositor

   Witold Filipowicz, Marina Chekulaeva

   Article

   Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

   Insert

   TNRC6C CED (TNRC6C Human)
   

   Tags

   NHA

   Expression

   Mammalian

   Mutation

   C-terminal effector domain (CED): aa 1369-1690 wi…

   Promoter

   CMV

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. L2096-GPA-BRET-Biosensor-Retrovirus-TD146

   Plasmid

   #222878

   Purpose

   Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with BRET (split NanoLuciferase reconstitution [11S/114 fragment]). WT Glycophorin A (GPA) scaffold.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-NanoLuc114-CyOFP1-T2A-FKBP-GPA-NanoLuc11S
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   MSCV LTR

   Available Since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-FKBP-ICUE3-NLS

   Plasmid

   #138255

   Purpose

   FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.

   Depositor

   Jin Zhang

   Article

   Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

   Insert

   FKBP-ICUE3-NLS
   

   Tags

   FKBP, Nuclear localization signal (NLS), cpVenus[…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. LAMP1-mCherry-FRB*

   Plasmid

   #207145

   Purpose

   Lysosome-targeted rapalog dimerizer

   Depositor

   Antony Galione

   Article

   Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

   Insert

   LAMP1 (LAMP1 Human)
   

   Tags

   FRB* (T2098L mutant of FKBP-rapamycin binding dom…

   Expression

   Mammalian

   Mutation

   Silent mutation

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAcUW51-gEgIL

   Plasmid

   #11622

   Depositor

   Pamela Bjorkman

   Article

   Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

   Insert

   HSV-1 glycoprotein E and HSV-1 glycoprotein I
   

   Tags

   His

   Expression

   Insect

   Mutation

   Dicistronic vector containing HSV-1 gE (KOS strai…

   Available Since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

6. XIAP (pBS0852)

   Plasmid

   #185282

   Purpose

   For the mammalian expression of the human protein XIAP. This is the positive control we used in our apoptosis assay.

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   XIAP (XIAP Human)
   

   Expression

   Mammalian

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-eYFP-FKBP-MAD1-WT

   Plasmid

   #114037

   Purpose

   Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

   Depositor

   Geert Kops

   Article

   Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

   Insert

   Human MAD1 (MAD1L1 Human)
   

   Tags

   eYFP-FKBP12

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-C1-FKBP-CYB5tail

   Plasmid

   #202686

   Purpose

   rapamycin-induced recruitment of proteins to ER

   Depositor

   Gerry Hammond

   Article

   Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

   Insert

   EGFP:FKBP:[GGSA]4GG:CYB5A(100-134) (CYB5A Human)
   

   Expression

   Mammalian

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLS2-YFPc

   Plasmid

   #87166

   Purpose

   fluorescence complementation assay

   Depositor

   Silke Robatzek

   Article

   Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

   Insert

   FLS2 (FLS2 Mustard Weed)
   

   Tags

   YFPc

   Promoter

   35s

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLS2-YFPn

    Plasmid

    #87165

    Purpose

    fluorescence complementation assay

    Depositor

    Silke Robatzek

    Article

    Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

    Insert

    FLS2 (FLS2 Mustard Weed)
    

    Tags

    YFPn

    Promoter

    35s

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. LBR-FKBP-GFP

    Plasmid

    #222413

    Purpose

    To express LBR with C-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

    Depositor

    Stephen Royle

    Article

    Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

    Insert

    LBR (LBR Human)
    

    Tags

    FKBP-GFP

    Expression

    Mammalian

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. FKBP-Stx3-mCitrine

    Plasmid

    #92428

    Purpose

    Expression of syntaxin-3 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx3 (Stx3 Rat)
    

    Tags

    FKBP (FK506 binding protein 12); allows for heter…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. FKBP-Stx4-mCitrine

    Plasmid

    #92427

    Purpose

    Expression of syntaxin-4 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx4 (Stx4 Rat)
    

    Tags

    FKBP (FK506 binding protein 12); allows for heter…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-mCherry-FKBP-TIAM1(DHPH)

    Plasmid

    #176138

    Purpose

    Heterodimerization with FRB (rapamycin), Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AfrLEA3m_mCh-2xFKBP (pBS1137)

    Plasmid

    #185321

    Purpose

    For the mammalian expression of the brine shrimp protein AfrLEA3m attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    AfrLEA3m
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pST_APOE_HUMAN_E4_47-300 (pBS0847)

    Plasmid

    #185279

    Purpose

    For the mammalian expression of the human protein APOE_HUMAN_E4_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    APOE_HUMAN_E4_47-300
    

    Expression

    Mammalian

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. CAHS2parrc-mCh-2xFKBP (pBS1109)

    Plasmid

    #185304

    Purpose

    For the mammalian expression of the tardigrade protein CAHS2parrc attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS2parrc
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEntr1a-mcherry-FKBP-TIAM1(DHPH)

    Plasmid

    #176135

    Purpose

    Heterodimerization with FRB (rapamycin), Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Entry vector

    Mutation

    See Depositor Comments Below

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pST_MAHS_RAMVA_81-236-FLAG (pBS0297)

    Plasmid

    #185328

    Purpose

    For the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236-FLAG. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    MAHS_RAMVA_81-236-FLAG
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLentiCRISPR V2 sgYME1L1 sg2

    Plasmid

    #244868

    Purpose

    Knockout of human YME1L1

    Depositor

    Alexis Jourdain

    Article

    Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

    Insert

    YME1L1 sgRNA (YME1L1 Human)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    Oct. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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