We narrowed to 4,182 results for: biorxiv

1. pAG415Gal_Gcn4 Aro3+GFP

   Plasmid

   #233002

   Purpose

   Galactose iduced expression of Gcn4 Aro3+GFP in yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 Aro3+
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   D103Y, N112T, L113Y, S117F, K140R

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAG415Gal_Gcn4 ILVtoED WGFP

   Plasmid

   #233007

   Purpose

   Galactose iduced expression of Gcn4 LVtoED W+GFPin yeast

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoED W+
   

   Use

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   N126W, I128E, V130E, V135E, L137D, I142D

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIVT-evoBxb1

   Plasmid

   #232747

   Purpose

   IVT template for making modRNA encoding evoBxb1

   Depositor

   Kate Galloway

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   Bxb1
   

   Use

   In vitro transcription

   Tags

   HA and SV40 NLS

   Expression

   Mutation

   V74A

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDest17_Gcn4 solvvol W

   Plasmid

   #231878

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 solvvol W

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 solvvol W
   

   Use

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

   Promoter

   T7

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDest17_Gcn4 solvvol

   Plasmid

   #231877

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 solvvol

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 solvvol
   

   Use

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

   Promoter

   T7

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDest17_Gcn4 SATtoG KtoR

   Plasmid

   #231861

   Purpose

   Bacterial expression of N-terminally 6His tagged Gcn4 SATtoG KtoR

   Depositor

   Tanja Mittag, Aseem Ansari

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 SATtoG KtoR
   

   Use

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Mutation

   S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

   Promoter

   T7

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pIVT-Bxb1-I87L

   Plasmid

   #232749

   Purpose

   IVT template for making modRNA encoding mutant Bxb1 modRNA (I87L)

   Depositor

   Kate Galloway

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   Bxb1
   

   Use

   In vitro transcription

   Tags

   HA and SV40 NLS

   Expression

   Mutation

   I87L

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOPINF HopF2

   Plasmid

   #228397

   Purpose

   Recombinant expression of His6-tagged Pseudomonas syringae pv tomato type III effector HopF2 in E. coli

   Depositor

   Lennart Wirthmueller

   Article

   Simranjit et al bioRxiv 2023.09.25.558804

   Insert

   HopF2 (hopF2 Pseudomonas syringae pv. tomato)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. JDW 1394 (pME-ZIC3-P2A-H2A-mCherry)

   Plasmid

   #232133

   Purpose

   A middle entry gateway clone containing V5 tagged human ZIC3 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   ZIC3 (ZIC3 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR4 HopU1

    Plasmid

    #228395

    Purpose

    pENTR4 plasmid with CDS of Pseudomonas syringae pv tomato type III effector HopU1 without stop codon for C-terminal epitope tags

    Depositor

    Lennart Wirthmueller

    Article

    Simranjit et al bioRxiv 2023.09.25.558804

    Insert

    HopU1
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Alanine inserted after start Methionine due to Nc…

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. JDW 1063 (pLenti6.2_CMV_V5_MAFB_P2A_H2A_mCherry)

    Plasmid

    #233545

    Purpose

    A CMV driven human MAFB followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    MAFB (MAFB Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. JDW 1052 (pME_V5_MAFB_P2A_H2A_mCherry)

    Plasmid

    #232129

    Purpose

    A middle entry gateway clone containing V5 tagged human MAFB and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    MAFB (MAFB Human)
    

    Use

    Gateway cloning

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCRISPEY-Z3-Kan

    Plasmid

    #232102

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPEY-Z3-Hyg

    Plasmid

    #232101

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TRE-pfv-Sapphire

    Plasmid

    #231957

    Purpose

    The plasmid containing TRE-pfv-Sapphire for the doxycycline-inducible expression of genetically encoded multimeric nanoparticles (GEM)

    Depositor

    Michael Shtutman

    Article

    Korunova et al bioRxiv [Preprint]. 2024 Nov 18:2024.11.

    Insert

    genetically encoded multimeric nanoparticles
    

    Use

    Lentiviral; Doxocycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. Bxb1-GT_TRE-mScarlet

    Plasmid

    #229796

    Purpose

    Bxb1-GT donor plasmid for doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mScarlet
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Bxb1-GA_CAG-mStayGold

    Plasmid

    #229802

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. Bxb1-GA_TRE-jRCaMP1b

    Plasmid

    #229798

    Purpose

    Bxb1-GA donor plasmid for doxycycline-inducible expression of jRCaMP1b calcium reporter.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    jRCaMP1b
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. act5C-mScarlet

    Plasmid

    #230906

    Purpose

    Ubiquitous expression of mScarlet in Drosophila melanogaster

    Depositor

    Michael Boutros

    Article

    Port et al bioRxiv 2024.11.26.625385

    Insert

    mScarlet
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    act5C

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. UAST-uMCas12a+nls2x

    Plasmid

    #230909

    Purpose

    inducible expression of Cas12a+ with Gal4.

    Depositor

    Michael Boutros

    Article

    Port et al bioRxiv 2024.11.26.625385

    Insert

    Cas12a+
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge