We narrowed to 2,718 results for: fas

1. pXPR_051_dCas9-KRAB-HA

   Plasmid

   #228936

   Purpose

   All-in-one dCas9-KRAB-MeCP2 plasmid for cloning of custom sgRNA

   Depositor

   Francisca Vazquez

   Article

   Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. IMPT-10314:GABBR2

   Plasmid

   #157851

   Purpose

   HA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expression

   Depositor

   Vadim Cherezov, Raymond Stevens

   Article

   Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

   Insert

   GABBR2 (GABBR2 Human)
   

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC3.1_CMV_oROS-G_LF(C199S)

   Plasmid

   #216112

   Purpose

   Expresses the loss-of-function mutations C199S of the genetically encoded green fluorescent hydrogen peroxide sensor oROS-G in mamalian cells.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.01.31.578117

   Insert

   oROS-G_LF(C199S)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28b_H6-Smt3-AesC-GFP

   Plasmid

   #234486

   Purpose

   Superfolder GFP C-terminally fused to His-Smt3-tagged AesC split intein

   Depositor

   Henning Mootz

   Article

   Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:

   Insert

   C-terminal Fragment of the Aes123 PolB1 Split Intein
   

   Tags

   Hexahistidine Tag, Superfolder Green Fluorescent …

   Expression

   Bacterial

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP

   Plasmid

   #158962

   Purpose

   Cre-dependent Cas9-GFP expression

   Depositor

   Michael Brand

   Article

   Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

   Inserts

   hsp70l promoter
   floxed DsRed cassette
   Cas9-GFP
   

   Use

   CRISPR and Cre/Lox

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1/Puro-CAG-ASAP2s

   Plasmid

   #101274

   Purpose

   Genetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)

   Depositor

   Francois St-Pierre

   Article

   Chamberland et al eLife. 2017 Jul 27;6. pii: e25690. doi:

   Insert

   ASAP2s
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. p(HA)HIF1alpha (P402A,P564A)

   Plasmid

   #52636

   Purpose

   mammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutation

   Depositor

   Eric Huang

   Article

   Kageyama et al FASEB J. 2004 Jun;18(9):1028-30. Epub 20

   Insert

   HIF1alpha P402A, P564A (HIF1A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   P402A, P564A

   Promoter

   CMV

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-syn-dLight1.3b

   Plasmid

   #135762

   Purpose

   For expression of the genetically encoded dopamine sensor dLight1.3b

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Has Service

   AAV9

   Insert

   dLight1.3b
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-dLight1.2

   Plasmid

   #111068

   Purpose

   To generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoter

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Has Service

   AAV1 and AAV5

   Insert

   dLight1.2
   

   Use

   AAV

   Tags

   Flag tag

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPR-HOT-P2A-Clover-BlastR

    Plasmid

    #138568

    Purpose

    Universal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJ

    Depositor

    Hans Clevers

    Article

    Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

    Insert

    P2A-clover-BlastR
    

    Use

    CRISPR

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tubulin-mGreenLantern

    Plasmid

    #164460

    Purpose

    Localization of mGreenLantern fluorescent protein to tubulin

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Tags

    Tubulin

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRDA355_sgCiPELO #2

    Plasmid

    #229020

    Purpose

    Expression of a CRISPRi doxycycline inducible guide targeting PELO

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    PELO gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. nLuc_AP4_TEVs_P3mS

    Plasmid

    #119299

    Purpose

    Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logic

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker
    

    Use

    Luciferase

    Tags

    Myc tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. AP4mS_PPVs_P3_cLuc

    Plasmid

    #119300

    Purpose

    Expresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split cLuc fused to P3coil-PPV cleavage site and autoinhibitory coil AP4mS
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Mito-mGreenLantern

    Plasmid

    #164461

    Purpose

    Localization of mGreenLantern fluorescent protein to mitochondria

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Tags

    Human COX8a first 29 amino acids

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. NLS-Laconic/pCMV-myc-nuc

    Plasmid

    #46307

    Purpose

    a nuclear-targeted, genetically-encoded sensor for lactate

    Depositor

    Luis Felipe Barros

    Article

    Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

    Insert

    NLS-Laconic
    

    Use

    Lactate biosensor

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCSN068

    Plasmid

    #101749

    Purpose

    Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Inserts

    Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
    KanMX marker expression cassette.
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Promoter

    Heterologous TEF1 promoter from A. gossypii. and …

    Available Since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. H2B-mGreenLantern

    Plasmid

    #164464

    Purpose

    Localization of mGreenLantern fluorescent protein to histone 2B

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Tags

    Human Histone 2B (D26G and V119I mutations)

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CMV

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CAG-mGreenLantern

    Plasmid

    #164469

    Purpose

    Constitutive CAG-driven expression of mGreenLantern in animals using AAV

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    CAG

    Available Since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-CMV_TetR-HA-NLS-P2A-BSD-Myc

    Plasmid

    #113899

    Purpose

    Mammalian constitutive expression of HA-tagged Tetracyclin repressor protein and Myc-tagged blasticidin deaminase

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    TetR-HA-NLS-P2A-BSD-Myc
    

    Use

    Lentiviral

    Tags

    HA-Myc

    Promoter

    CMV-MIE

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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