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Plasmid#157851PurposeHA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expressionDepositorInsertGABBR2 (GABBR2 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrinAvailable sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV_TetR-HA-NLS-P2A-BSD-Myc
Plasmid#113899PurposeMammalian constitutive expression of HA-tagged Tetracyclin repressor protein and Myc-tagged blasticidin deaminaseDepositorInsertTetR-HA-NLS-P2A-BSD-Myc
UseLentiviralTagsHA-MycExpressionMutationPromoterCMV-MIEAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-dLight1.1
Plasmid#111066PurposeTo generate Adeno-Associated viruses for expression of dLight1.1 under synapsin promoterDepositorHas ServiceAAV1InsertdLight1.1
UseAAVTagsFlag tagExpressionMutationPromoterAvailable sinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-dLight1.3b
Plasmid#135762PurposeFor expression of the genetically encoded dopamine sensor dLight1.3bDepositorHas ServiceAAV9InsertdLight1.3b
UseAAVTagsFlag tagExpressionMammalianMutationPromoterSynapsinAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGY577
Plasmid#166663PurposeLight-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombinationDepositorInsertLiCre
UseCre/Lox and LentiviralTagsExpressionMammalianMutationCre: E340A, D341APromoterCMVAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSN067
Plasmid#101748PurposeSingle copy yeast plasmid expressing Cpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsCpf1 from Lachnospiraceae bacterium ND2006 (LbCpf1) codon optimized for expression in S. cerevisiae.
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastMutationPromoterHeterologous TEF1 promoter from A. gossypii. and …Available sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
ER-mGreenLantern
Plasmid#164463PurposeLocalization and retention of mGreenLantern fluorescent protein in the ERDepositorInsertmGreenLantern
UseTagsCalreticulin and KDELExpressionMammalianMutationClover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…PromoterCMVAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-dLight1.2
Plasmid#111068PurposeTo generate Adeno-Associated viruses for expression of dLight1.2 under synapsin promoterDepositorHas ServiceAAV1 and AAV5InsertdLight1.2
UseAAVTagsFlag tagExpressionMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBABR IRFP-Bcd-LEXY
Plasmid#182595PurposeDrosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.DepositorInsertBcd (bcd Fly)
UseTagsIRFP and LEXYExpressionInsectMutationPromotermaternal tubulinAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Designed VEGF-binding scFv G6des13
Plasmid#110213PurposeYeast Surface Display of G6des13DepositorInsertG6des13
UseTagscmyc tagExpressionYeastMutationL A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95FPromoterAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLPCX2-HA-GATA3 FL
Plasmid#89835PurposeExpress GATA3 in mammalian cellsDepositorInsertGata3 (Gata3 Mouse)
UseRetroviralTagsHAExpressionMutationPromoterCMVAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Designed VEGF-binding scFv G6des1
Plasmid#110212PurposeYeast Surface Display of G6des1DepositorInsertG6des1
UseTagscmyc tagExpressionYeastMutationL A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…PromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
FUT8-Dox
Plasmid#124631PurposeDoxycycline-inducible FUT8 expression in mammalian cellsDepositorInsertsAlpha-(1,6)-fucosyltransferase
Tetracycline repressor A3 mutant
TagFP635
UseTagsExpressionMammalianMutationPromoterTRE-tight and hEF1aAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator
Plasmid#158585PurposeExpresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiaeDepositorInsertmembrane localization signal-tagBFP-wrmScarlet11
UseTagstagBFPExpressionYeastMutationGGGS linkerPromoterTEFAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSN068
Plasmid#101749PurposeSingle copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsCpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastMutationPromoterHeterologous TEF1 promoter from A. gossypii. and …Available sinceJan. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-ASAP2s
Plasmid#101274PurposeGenetically encoded voltage indicator ASAP2s expressed under strong mammalian promoter (CAG)DepositorInsertASAP2s
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-POLR2A-CTD-mCherry
Plasmid#185011PurposeBacterial Expression of 6xHis C-terminal domain of POLR2A mCherry Fusion ProteinDepositorInsertPOLR2A (Polr2a Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2+8 Flag2AUF1(p40)
Plasmid#135945PurposeExpresses AUF1p40 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.DepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
UseTagsFlag2ExpressionMammalianMutationPromoterCMV and SP6Available sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+8 Flag2AUF1(p42)
Plasmid#135946PurposeExpresses AUF1p42 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.DepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
UseTagsFlag2ExpressionMammalianMutationPromoterCMV and SP6Available sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ASAP2s
Plasmid#101276PurposeGenetically encoded voltage indicator (GEVI) ASAP2s in AAV vector for neuron-specific expression using the promoter hSyn1DepositorInsertASAP2s
UseAAVTagsExpressionMammalianMutationPromoterhSyn (human synapsin)Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only