We narrowed to 15,088 results for: pET

1. pET21b-Tipin-His

   Plasmid

   #23122

   Depositor

   Aziz Sancar

   Article

   Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

   Insert

   Tipin (TIPIN Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Jan. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pETM-30-GST-PfAgo_F

   Plasmid

   #146265

   Purpose

   Bacterial Expression of PfAgo

   Depositor

   Elisa Izaurralde

   Insert

   PfAgo
   

   Expression

   Bacterial

   Mutation

   one silent T1704C and one non silent mutation A90…

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. BioBrick pET-24a(+)

   Plasmid

   #117802

   Purpose

   BioBrick pET vector for protein overexpression in E. coli

   Depositor

   Anthony Forster

   Article

   Du et al Biotechnol Bioeng. 2009 Dec 15;104(6):11

   Type

   Empty backbone

   Use

   Escherichia coli

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-pro-sumo1

   Plasmid

   #25101

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   pro-SUMO1 (SUMO1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Changed Serine 2 to Alanine

   Available Since

   June 22, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pET-Sac-Aβ(M1-42/C18C33)

   Plasmid

   #173759

   Purpose

   Expresses the AβC18C33 peptide.

   Depositor

   James Nowick

   Article

   Zhang et al Biochemistry. 2022 Feb 15;61(4):252-264.

   Insert

   Aβ(M1-42/C18C33) (APP Human)
   

   Expression

   Bacterial

   Mutation

   changed valine 18 to cysteine; changed glycine 33…

   Promoter

   T7

   Available Since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-RBP-LOV2

   Plasmid

   #185792

   Purpose

   Encodes Light-oxygen-voltage-sensing domain 2 linked to RNA binding peptide (on N terminal end) via GS-linker

   Depositor

   Lizhong He

   Article

   Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

   Insert

   RBP-LOV2
   

   Tags

   10xHis, TEV protease cleavage site after His Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b

   Plasmid

   #122850

   Purpose

   Expresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coli

   Depositor

   Martin Grininger

   Article

   Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

   Insert

   m(KS_MAT)_mouseL_ACP (Fasn Mouse)
   

   Tags

   His8 tag and Twin-Strep tag

   Expression

   Bacterial

   Mutation

   wildtype

   Promoter

   T7 promoter

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28b-6His-AcpS

   Plasmid

   #145376

   Purpose

   Expression of E. coli AcpS

   Depositor

   Robert Tomko

   Article

   Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

   Insert

   AcpS (acpP E. coli)
   

   Tags

   6-His

   Expression

   Bacterial

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pET sfGFP(150TAG)

   Plasmid

   #133455

   Purpose

   expresses sfGFP with an unnatural amino acid at the 150th position in bacterial cells

   Depositor

   Yu-Hsuan Tsai

   Article

   Williams et al Amino Acids. 2021 Jan;53(1):89-96. doi:

   Insert

   sfGFP
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCyPet-Rac1(Q61L)

    Plasmid

    #22783

    Depositor

    Klaus Hahn

    Article

    Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

    Insert

    Rac1 (Q61L) (RAC1 Human, A. victoria)
    

    Tags

    CyPet

    Expression

    Mammalian

    Mutation

    Rac1 Q61L.

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pETM-11-6His-DmBelle_B

    Plasmid

    #145950

    Purpose

    Bacterial Expression of DmBelle

    Depositor

    Elisa Izaurralde

    Insert

    DmBelle (bel Fly)
    

    Expression

    Bacterial

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-Sangamo-HIS

    Plasmid

    #40787

    Purpose

    for e. coli expression, Golden Gate Compatible TALEN incorporated with C Terminal 6xHIS Tag, Codon Optimized FokI

    Depositor

    Gang Bao

    Insert

    Transcription Activator Like Effector Nuclease
    

    Use

    TALEN

    Tags

    C Terminal 6xHIS

    Expression

    Bacterial

    Mutation

    +63 C Terminus,Codon Optimized FokI Endonuclease

    Promoter

    T7

    Available Since

    Dec. 12, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pET51b(+)-SNAP-CLIP

    Plasmid

    #127180

    Purpose

    Bacterial expression of the fused self-labeling tags SNAP-tag and CLIP-tag

    Depositor

    Kai Johnsson

    Article

    Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

    Insert

    SNAP-CLIP
    

    Tags

    HisTag and StrepTag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-51b(+)-Caprola_05

    Plasmid

    #194666

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_05 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_05
    

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21-pelB-LaM-8

    Plasmid

    #172772

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-8
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETDUET-GST AO7REwt

    Plasmid

    #213935

    Purpose

    GST tagged AO7 encoding aa 126-258 for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    AO7 cloned into the first multiple cloning site o…

    Promoter

    T7 promoter-1

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-Bst-LF-Idaho

    Plasmid

    #170469

    Purpose

    Expresses His-tagged Bst-LF-Idaho in E. coli

    Depositor

    Jonathan Schmid-Burgk

    Insert

    Bst-LF-Idaho
    

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM-60-NusA-PfAgo_F

    Plasmid

    #146270

    Purpose

    Bacterial Expression of PfAgo

    Depositor

    Elisa Izaurralde

    Insert

    PfAgo
    

    Expression

    Bacterial

    Mutation

    one non silent mutation A907G (I303V) and one sil…

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. ScSpe1 pET-20b(+)

    Plasmid

    #117145

    Purpose

    Expresses the His tagged Saccharomyces cerevisiae Ornithine Decarboxylase in Escherichia coli BL21

    Depositor

    Markus Ralser

    Article

    Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

    Insert

    SPE1
    

    Tags

    Histidine

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21a-C2m2-mKate

    Plasmid

    #208765

    Purpose

    To express C2m2-mKate in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2m2-mKate (Mfge8 Mouse)
    

    Tags

    mKate

    Expression

    Bacterial

    Mutation

    Mutated 24 and 45 lysine to asparagine in C2 doma…

    Promoter

    T7 promoter

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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