161,256 results

1. lentiCRISPRi(v1)-Blast

   Plasmid

   #170067

   Purpose

   Expresses dCas9 repressor KRAB-dCas9 and blasticidin resistance

   Depositor

   Neville Sanjana

   Article

   Morris et al Science. 2023 May 19;380(6646):eadh7699.

   Insert

   KRAB-dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2A and 2xHA tag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJC6-EV

   Plasmid

   #163454

   Purpose

   empty vector control for pLJC6

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGex-Cav1

   Plasmid

   #33364

   Depositor

   Ambra Pozzi

   Article

   Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

   Insert

   Cav1 (CAV1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pRS404-PTEF-Ago1

   Plasmid

   #22313

   Depositor

   David Bartel

   Article

   Drinnenberg et al Science. 2009 Sep 10. ():.

   Insert

   AGO1
   

   Use

   Integrating plasmid

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEJS2601_AAV-Nme2-ABE8e-i1-U6-2xSapI

   Plasmid

   #201683

   Purpose

   All-in-one AAV plasmid expressing Nme2-ABE8e-i1 and U6 sgRNA cassette. SapI enables cloning new spacers.

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

   Insert

   Nme2-ABE8e-i1
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. GAPDH-CPG-TV

   Plasmid

   #70148

   Purpose

   a targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyA

   Depositor

   Rudolf Jaenisch

   Article

   Stelzer et al Cell. 2015 Sep 24;163(1):218-29. doi: 10

   Insert

   5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh
   

   Use

   Mouse Targeting and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pscAAV-GFP

   Plasmid

   #32396

   Depositor

   John T Gray

   Article

   Gray et al Methods Mol Biol. 2011;807:25-46.

   Insert

   GFP
   

   Use

   AAV; Adeno-associated virus

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO-GAlphasL-RLuc8

   Plasmid

   #140981

   Purpose

   Encodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GAlphasL-RLuc8 (GNAS Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-HisMBP

   Plasmid

   #11085

   Depositor

   David Waugh

   Article

   Nallamsetty et al Protein Sci. 2005 Dec . 14(12):2964-71.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Tac (lactose/IPTG inducible)

   Available since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMMB67EH-CFP

    Plasmid

    #90103

    Purpose

    CFP control expression plasmid

    Depositor

    Samuel Miller

    Article

    Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

    Insert

    CFP
    

    Use

    Broad-host range

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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