We narrowed to 3 results for: 74707

1. pBluescriptSKII+ U6-sgRNA(F+E) empty

   Plasmid

   #74707

   Purpose

   Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

   Depositor

   Eugene Yeo

   Article

   Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

   Insert

   U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
   

   Expression

   Mammalian

   Available Since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. Pb mD3S4-dN

   Plasmid

   #180252

   Purpose

   P. berghei D3 (502-617) construct with N516T, S533N and N539Q mutations to abolish N-glycosylation was used for crystallization.

   Depositor

   Timothy Springer

   Article

   Feng et al Elife. 2021 Dec 23;10. pii: 74707. doi:

   Insert

   D3 of P. berghei HAP2 with N-glycosylation sites mutated
   

   Tags

   6His

   Expression

   Mammalian

   Mutation

   N516T, S533N and N539Q

   Available Since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TetO::EGFP-hMeikin(8A mut)

   Plasmid

   #174707

   Purpose

   Doxycycline inducible expression of human Meikin with 8 alanine mutations that eliminate Plk1 binding

   Depositor

   Iain Cheeseman

   Article

   Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

   Insert

   Meikin
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S175A, T176A, T180A, S181A, S196A, T251A, T264A, …

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge