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Plasmid#91720PurposeIntegration-proficient vector for Mycobacterium smegmatis, Mycobacterium tuberculosis, and bacille Calmette-GuerinDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 17, 2017AvailabilityAcademic Institutions and Nonprofits only
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MtMscL
Plasmid#79028PurposeMycobacterium tuberculosis MscLDepositorInsertMtMscL
UseTags10x Histidine TagExpressionMutationPromoterT7 PromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHLEGM2
Plasmid#24781DepositorInsertPtet-Gfp-LAA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHLEGM3
Plasmid#24782DepositorInsertPtet-Gfp-ASV
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHLEGM4
Plasmid#24783DepositorInsertPtet-Gfp-ASV
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pNIC28-Bsa4-ICL1
Plasmid#105822PurposeExpression of Mycobacterium tuberculosis icl1 recombinant proteinDepositorInserticl1
UseTagsHis tag with TEV cleavageExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAPA3
Plasmid#24777DepositorTypeEmpty backboneUseIntegrating vectorTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pfbiRS
Plasmid#67283PurposeExpresses the fbiC gene from Mycobacterium smegmatisDepositorInsertfbiC gene from Mycobacterium smegmatis
UseTagspolyhistidineExpressionMammalianMutationnon knownPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGMCS-0X-P38-PanBmGFP
Plasmid#27127DepositorInsertPanB
UseTagsLinker (SGSGSG) and mGFPExpressionBacterialMutationPromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBUN250
Plasmid#60672Purposeoverexpression of M. smegmatis ddl gene in MycobacteriumDepositorInsertddl
UseE.coli-mycobacterium shuttle vectorTagsExpressionMutationPromoterBCG Hsp60Available sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJK126
Plasmid#73337PurposeEncodes Mycobacterium tuberculosis FdxB (C-terminal flavoprotein reductase domain)DepositorInsertelectron transfer flavoprotein
UseTagsHis6ExpressionBacterialMutationencodes residues 328-685PromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK125
Plasmid#73336PurposeEncodes Mycobacterium tuberculosis FdxB (N-terminal fatty acid desaturase domain)DepositorInsertelectron transfer flavoprotein
UseTagsHis6ExpressionBacterialMutationencodes residues 1-327PromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMycoFos
Plasmid#84577PurposeShuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium. Copy number is controllable by arabinose in E.coli.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBUN276
Plasmid#60673Purposeoverexpression of M. tuberculosis ddl gene in MycobacteriumDepositorInsertddl
UseE.coli-mycobacterium shuttle vectorTags6 codons of M. bovis BCG hsp60ExpressionMutationPromoterBCG Hsp60Available sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
Rv2002 M3/pET-28b(+)N-tag
Plasmid#13057DepositorInsert3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMV306DIG13+FFlucRT
Plasmid#49998PurposeIntegrase removed to increase stability of the plasmid in mycobacteriaDepositorInsertG13 promoter + Firefly luciferase Red Thermostable
UseMycobacteria integrating vectorTagsExpressionBacterialMutationCodon optimized for Mycobacterium tuberculosis wi…PromoterG13Available sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMV306G13+FFlucRT
Plasmid#49997PurposeIntegrating vector with an improved Red-shifted Thermostable Firefly LuciferaseDepositorInsertG13 promoter + Firefly luciferase Red Thermostable
UseMycobacteria integrating vectorTagsExpressionBacterialMutationCodon optimized for Mycobacterium tuberculosis wi…PromoterG13 from M. marinumAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBUN279
Plasmid#60674Purposeintegration of M. smegmatis ald gene in Mycobacterium using mycobacteriophage L5 attB siteDepositorInsertald
UseE.coli-mycobacterium integration-proficient vectorTagsExpressionMutationPromoterAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
MtMscL delta49
Plasmid#118342PurposeM. tuberculosis MscL (delta49) in pET19bDepositorInsertMscL (mscL Mycobacterium tuberculosis)
UseTags6x His Tag and Thrombin Cleavage siteExpressionBacterialMutationChanged Glutamic Acid (Glu) 102 to Stop CodonPromoterT7 PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only