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Addgene
Showing: 1 - 10 of 10 results
  1. AICSDP-63: ACTN2-mEGFP

    Plasmid
    #124607
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette
    Insert
    ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. Ad:2CMV_A2R2-N25K3

    Plasmid
    #122242
    Purpose
    Expresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone
    Depositor
    Inserts
    C-terminal end of Nesprin-3 (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    Use
    Adenoviral
    Tags
    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  3. Ad:2CMV_A2R2-N25Ctr

    Plasmid
    #122243
    Purpose
    Expresses a dominant negative Kash control, that lacks the actual Kash domain for LINC complex integration, in addition to fluorescently tagged (mRuby2) α-Actinin-2 on an adenoviral backbone
    Depositor
    Inserts
    C-terminal end of Nesprin-3 w/o Kash (Syne3 Mouse)
    Actinin alpha 2 (Actn2 Mouse)
    Use
    Adenoviral
    Tags
    Signal Peptide (TOR1A, NM_000113.2), mNeptune2.5,…
    Expression
    Mammalian
    Mutation
    deleted Kash domain (GGCGAGGAGGAGCGCAGCTGCGCCCTGG…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  4. tdEos-Alpha-Actinin2-N-18

    Plasmid
    #57577
    Purpose
    Localization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Insert
    Alpha-Actinin2 (ACTN2 Human)
    Use
    Tags
    tdEos
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
  5. mCherry-Alpha-Actinin2-N-18

    Plasmid
    #54974
    Purpose
    Localization: Alpha Actinin, Excitation: 587, Emission: 610
    Depositor
    Insert
    Alpha-Actinin2 (ACTN2 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 10 of 10 results