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Showing: 1 - 20 of 36 results

  3. ATX3-D1

    Plasmid
    #184246
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain and UIM1 fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    Ataxin-3 Josephin domain and UIM1 (ATXN3 Human)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Premature stop codon N263X
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    184246-plasmid-map-sequence-id-370314

    Enlarge

  5. pLJM1-Flag-Rheb-4Rs

    Plasmid
    #165022
    Purpose
    expression of Flag-Rheb-4Rs mutant protein in mammalian cells (the lysine residues on the position 109, 135, 151 and 178 on Rheb protein are mutated to arginines)
    Depositor
    Ken Inoki
    Article
    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d
    Insert
    Rheb-4Rs (RHEB Human)
    Use
    Lentiviral
    Tags
    flag
    Expression
    Mammalian
    Mutation
    change lysine residues on position 109, 135, 151,…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    165022-plasmid-map-sequence-id-326717

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  6. pLJM1-Flag-Rheb-12Rs

    Plasmid
    #165023
    Purpose
    expression of Flag-Rheb-12Rs mutant protein in mammalian cells (all the lysine residues on Rheb except the K19 and K120 are mutated to arginines).
    Depositor
    Ken Inoki
    Article
    Yao et al Mol Cell. 2020 Nov 5;80(3):437-451.e6. d
    Insert
    Rheb-12Rs (RHEB Human)
    Use
    Lentiviral
    Tags
    flag
    Expression
    Mammalian
    Mutation
    All the lysine residues except K19 and K120 are c…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    165023-plasmid-map-sequence-id-326718

    Enlarge

  8. ATX3-13Q

    Plasmid
    #184248
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Synthetic, Human)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    C-terminal codon optimization for protein expres…
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    184248-plasmid-map-sequence-id-370322

    Enlarge

  9. pcDNA3-myc-Ataxin3Q28

    Plasmid
    #22125
    Depositor
    Henry Paulson
    Insert
    Ataxin 3 (ATXN3 Human)
    Use
    Tags
    myc
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  10. pFLAG-6a-Ataxin3Q22

    Plasmid
    #22126
    Depositor
    Henry Paulson
    Insert
    Ataxin 3 (ATXN3 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  13. ATX3-77Q

    Plasmid
    #184249
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    Ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track (ATXN3 Synthetic, Human)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Codon optimization for protein expression in BL2…
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    184249-plasmid-map-sequence-id-370367

    Enlarge

  14. ATX3-77Q-R388G

    Plasmid
    #184251
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-R388G fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 77Q in the Poly-Q track and point mutation R388G (ATXN3 Synthetic, Human)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    C-terminal codon optimization for protein expres…
    Promoter
    T7 promoter
    Availability
    Academic Institutions and Nonprofits only
    184251-plasmid-map-sequence-id-409179

    Enlarge

  15. ATX3 13Q- I77K Q78K W87K

    Plasmid
    #185908
    Purpose
    Expresses ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-I77K, Q78K,W87K (N-terminal ) fused with His-Tag and TEV cleavage sequence (C-terminal).
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track and point mutations I77K, Q78K and W87K (ATXN3 Synthetic, Human)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Point mutations in Atx3 gene I77K Q78K and W87K
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    185908-plasmid-map-sequence-id-370341

    Enlarge

  16. pFLAG-6a-Ataxin3Q22-UIM*

    Plasmid
    #22128
    Depositor
    Henry Paulson
    Insert
    Ataxin 3 (ATXN3 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    UIM 1,2,3 SA-DG
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  17. ATAXIN3:1-361_BV

    Plasmid
    #209732
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    Ataxin 3 (ATXN3 Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Polyhedrin
    Availability
    Academic Institutions and Nonprofits only
    209732-plasmid-map-sequence-id-414195

    Enlarge

  18. ataxin3:1-431_Q79_BV

    Plasmid
    #209733
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    Ataxin 3 (ATXN3 Human)
    Use
    Tags
    6xHis-SBP-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Polyhedrin
    Availability
    Academic Institutions and Nonprofits only
    209733-plasmid-map-sequence-id-414202

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Showing: 1 - 20 of 36 results