AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR
Plasmid
#60231
Purpose
Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
Depositor
Inserts
sgRNA
Cre recombinase
EGFP
Cre recombinase
EGFP
Use
AAV, CRISPR, Cre/Lox, and Mouse Targeting
Tags
Cre-HA-P2A and EGFP-KASH
Expression
Mammalian
Mutation
Promoter
U6 and hSyn
Availability
Academic Institutions and Nonprofits only