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pcDNA5 FRT TO-EGFP-G3BP1-S149A

Plasmid

#136004

Purpose

DOX-inducible S149A G3BP1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1

Use

Tags

EGFP

Expression

Mammalian

Mutation

S149A

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO-EGFP-G3BP1-DeltaRBP

Plasmid

#136005

Purpose

DOX-inducible G3BP1 with RNA binding domains deleted inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1

Use

Tags

EGFP

Expression

Mammalian

Mutation

C-term of G3BP1 Deleted for Delta RBP

Promoter

Availability

Academic Institutions and Nonprofits only

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pCI-neo-His-MS2BP-G3BP1-WT

Plasmid

#136008

Purpose

WT G3BP1 inserted with MS2BP tagged on the N-terminus to use with the Tethering Assay (MS2)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1

Use

Tags

MS2BP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

Plasmid

#196196

Purpose

homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)

Use

Homology repair donor

Tags

V5-APEX2-mScarlet-I

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCI-neo-His-MS2BP-G3BP1-S149A

Plasmid

#136009

Purpose

S149A G3BP1 inserted with MS2BP tagged on the N-terminus to use with the Tethering Assay (MS2)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1

Use

Tags

MS2BP

Expression

Mammalian

Mutation

S149A

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
psi-CHECK3

Plasmid

#136010

Purpose

psi-CHECK2 plasmid modified so both Renilla and Firefly luciferase contain introns

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pci-EGFP-UPF1-WT

Plasmid

#135994

Purpose

WT UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pci-EGFP-UPF1-R615A

Plasmid

#135995

Purpose

R615A UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1

Use

Tags

EGFP

Expression

Mammalian

Mutation

R615A

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pci-EGFP-UPF1-DEAA

Plasmid

#135996

Purpose

DEAA UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1

Use

Tags

EGFP

Expression

Mammalian

Mutation

DE647AA

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-lambdaN-UPF1-WT

Plasmid

#136006

Purpose

WT UPF1 inserted with lambdaN tagged on the N-terminus to use with the Tethering Assay (BoxB)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1

Use

Tags

LambdaN

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
psiCHECK3-Unstructured

Plasmid

#136025

Purpose

Artificial Unstructured 3'UTR inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

No gene (Artificial)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PLKO.1-Scrambled

Plasmid

#136035

Purpose

Scrambled shRNA (negative control) inserted into the PLKO.1 plasmid (CCTAAGGTTAAGTCGCCCTCG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

None (Scrambled)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
PLKO.1-UPF1-CDS

Plasmid

#136037

Purpose

UPF1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (AAGACACCTATTACACGAAGG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
PLKO.1-UPF2-CDS

Plasmid

#136040

Purpose

UPF2 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (GCGTTATGTTTGGTGGAAGAA)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
PLKO.1-UPF3A-CDS

Plasmid

#136043

Purpose

UPF3A shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (GCAGAAACCATTCTAAAGAAA)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF3A

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
PLKO.1-UPF3B-CDS

Plasmid

#136045

Purpose

UPF3B shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (GAAGCCTTGTTCCGATCTAAT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF3B

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
PLKO.1-STAU1-CDS

Plasmid

#136048

Purpose

STAU1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CGAGTAAAGCCTAGAATCAAA)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

STAU1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
PLKO.1-STAU2-CDS

Plasmid

#136050

Purpose

STAU2 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (GATATGAACCAACCTTCAA)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

STAU2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
PLKO.1-REG1-CDS

Plasmid

#136053

Purpose

REG1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (TATGGGATCAAGTGCCGATTC)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

REG1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
PLKO.1-CAPRIN1-CDS

Plasmid

#136054

Purpose

CAPRIN1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CCTCAGCAGAACACTGGATTT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

CAPRIN1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge