Addgene

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pCS2FLAG

Plasmid

#16331

Depositor

Peter Klein

Insert

Flag tag

Use

Vertebrate expression

Tags

Flag

Expression

Mutation

Flag tag inserted into pCS2+ polylinker

Promoter

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pCS2+8 Flag3HuR

Plasmid

#135950

Purpose

Expresses HuR in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Use

Tags

Flag3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2xVlk1-3xFlag

Plasmid

#172773

Purpose

mRNA synthesis for injection into embryos

Depositor

Edward De Robertis

Article

Ding et al Dev Biol. 2017 Jun 15;426(2):176-187. do

Insert

Vlk1 (pkdcc.1.S Frog)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2DY-EF

Plasmid

#221716

Purpose

Expression of zebrafish Ago2 with D651E and Y680F mutations and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2DY-EF (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

D651E and Y680F in active site

Promoter

simian CMV IE94

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2

Plasmid

#221712

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+8 Flag2AUF1(p45)

Plasmid

#135947

Purpose

Expresses AUF1p45 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2

Expression

Mammalian

Mutation

Promoter

CMV and SP6

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-hDKK3-flag

Plasmid

#15496

Depositor

Sergei Sokol

Article

Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

Insert

DKK3 (DKK3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

See note below

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

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pCS2-LRP6-3XFlag

Plasmid

#180144

Purpose

flag tagged LRP6

Depositor

Edward De Robertis

Article

Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

Insert

Human LRP6 (LRP6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SP6

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+8 Flag2AUF1(p37)

Plasmid

#135944

Purpose

Expresses AUF1p37 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2 Flag-JunB

Plasmid

#29687

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

jun B oncogene (JUNB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-hDKK1-flag

Plasmid

#15494

Depositor

Sergei Sokol

Article

Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

Insert

DKK1 (DKK1 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pCS2+ Dkk1-flag

Plasmid

#16690

Depositor

Xi He

Article

Semënov et al Curr Biol. 2001 Jun 26. 11(12):951-61.

Insert

Dkk1 (DKK1 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+8 Flag2AUF1(p40)

Plasmid

#135945

Purpose

Expresses AUF1p40 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2

Expression

Mammalian

Mutation

Promoter

CMV and SP6

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+8 Flag2AUF1(p42)

Plasmid

#135946

Purpose

Expresses AUF1p42 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2

Expression

Mammalian

Mutation

Promoter

CMV and SP6

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2-hDKK2-flag

Plasmid

#15495

Depositor

Sergei Sokol

Article

Krupnik et al Gene. 1999 Oct 1. 238(2):301-13.

Insert

DKK2 (DKK2 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pCS2+Flag-XTCF3-HA

Plasmid

#32998

Depositor

Sergei Sokol

Article

Hikasa et al Dev Cell. 2010 Oct 19;19(4):521-32.

Insert

XTCF3 (tcf3 Frog)

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

sCMV IE94

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pCS2 xRor2-Flag

Plasmid

#67164

Purpose

mammalian expression / in vitro transcription

Depositor

Alexandra Schambony

Article

Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

Insert

Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)

Use

In vitro transcription

Tags

FLag

Expression

Mammalian

Mutation

Promoter

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pCS2 3xFLAG FEM1A

Plasmid

#231001

Purpose

Transient overexpression of mouse FEM1A in mammalian cells

Depositor

Michael Rape

Article

Manford et al Cell. 2021 Oct 14;184(21):5375-5390.e16.

Insert

FEM1A (Fem1a Mouse)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ 3xFLAG-FEM1B WT

Plasmid

#230993

Purpose

Transient overexpression of mouse FEM1B in mammalian cells

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Insert

FEM1B (Fem1b Mouse)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-FLAG3-drAgo2D-A

Plasmid

#221713

Purpose

Expression of zebrafish Ago2 with D683A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-A (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Expression

Mutation

D683A in active site

Promoter

simian CMV IE94

Available since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge