Addgene

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pDF850

Plasmid

#228713

Purpose

Mammalian expression of EVOLVEpro PsaCas12f (epPsaCas12f)

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Science 0, eadr6006

Insert

epPsaCas12f

Use

CRISPR

Tags

Expression

Mammalian

Mutation

I178A/K333V/K454P

Promoter

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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SAPDF-pET21a(+)

Plasmid

#12649

Depositor

SeWon Suh

Article

Yoon et al Proteins. 2004 Nov 15. 57(3):639-42.

Insert

SA Peptide deformylase

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pDF1b-iBOP

Plasmid

#229976

Purpose

Rhamnose inducible expression of Bambusa oldhamii PAL gene

Depositor

Christie Peebles

Article

Hunstiger et al Biotechnol. Prog. 2024;e3512.

Inserts

Prha Rhamnose Inducible Promoter
Bambusa oldhamii PAL

Use

Tags

Expression

Bacterial

Mutation

Promoter

Prha and n/a

Available since

March 11, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDF1b-iNTP

Plasmid

#229977

Purpose

Rhamnose inducible expression of Nicotiana tabacum PAL 4 gene

Depositor

Christie Peebles

Article

Hunstiger et al Biotechnol. Prog. 2024;e3512.

Inserts

Prha Rhamnose Inducible Promoter
Nicotiana tabacum PAL 4

Use

Tags

Expression

Bacterial

Mutation

Promoter

Prha and n/a

Available since

March 11, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDF1b-iRGP

Plasmid

#229978

Purpose

Rhamnose inducible expression of Rhodotorula glutinis RgPAL gene

Depositor

Christie Peebles

Article

Hunstiger et al Biotechnol. Prog. 2024;e3512.

Inserts

Prha Rhamnose Inducible Promoter
Rhodotorula glutinis RgPAL

Use

Tags

Expression

Bacterial

Mutation

Promoter

Prha and n/a

Available since

March 11, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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AAEL009024 PDF R

Plasmid

#115408

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

AAEL009024 PDF R (LOC5571400 Aedes aegypti)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCasper_Pdf-LexA::VP16

Plasmid

#33259

Depositor

Michael Rosbash

Article

Shang et al Proc Natl Acad Sci U S A. 2008 Dec 16;10

Insert

LexA::VP16-SV40 3'UTR (lexA E. coli)

Use

Tags

Expression

Insect

Mutation

Promoter

Pdf

Available since

Dec. 12, 2011

Availability

Academic Institutions and Nonprofits only
PDF1::mCitrine-MBD

Plasmid

#115222

Purpose

Microtubule visualization

Depositor

Jan Traas

Article

Armezzani et al Development. 2018 Jun 4;145(11). pii: de

Insert

Microtubule binding domain

Use

Tags

mCitrine

Expression

Plant

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pDF0317 HvsCas7-11 mammalian expression

Plasmid

#172511

Purpose

Encodes human codon optimized HvsCas7-11 protein alone for expression in mammalian cells

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

HvsCas7-11 mammalian expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLNCX2-CFP-VGPDFGR-YFP

Plasmid

#59587

Purpose

Encodes a granzyme B FRET reporter

Depositor

Tim Mitchison

Article

Choi et al Proc Natl Acad Sci U S A. 2013 Apr 16;11

Insert

ECFP-VGPDFGR-Venus

Use

Retroviral

Tags

ECFP and Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pDF0159 pCMV - huDisCas7-11 mammalian expression

Plasmid

#172507

Purpose

Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 mammalian expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0234 pCMV - huDisCas7-11 dead mutant

Plasmid

#172509

Purpose

Encodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cells

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 dead mutant

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0352 HvsCas7-11 with C-term Nes mammalian expression

Plasmid

#172516

Purpose

Encodes human codon optimized HvsCas7-11 protein with C-terminal NES tag for expression in mammalian cells

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

C-term Nes HvsCas7-11 mammalian expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0387 pcmv-hudiscas7-11-r978-gggs-r1294

Plasmid

#186984

Purpose

mammalian expression of cas7-11-r978-gggs-r1294

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-r978-gggs-r1294

Use

Tags

Expression

Mammalian

Mutation

r978-gggs-r1294 deletion

Promoter

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDF0237 pCMV - dead huDisCas7-11 - NES - huADAR2

Plasmid

#172510

Purpose

Encodes human codon optimized catalytically dead DisCas7-11-NES-huADAR2 fusion protein alone for expression in mammalian cells

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - dead huDisCas7-11 - NES - huADAR2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0386 pcmv-hudiscas7-11-s1006-gggs-d1221

Plasmid

#186983

Purpose

mammalian expression of cas7-11-s1006-gggs-d1221

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-s1006-gggs-d122

Use

Tags

Expression

Mammalian

Mutation

s1006-gggs-d1221 deletion

Promoter

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDF0584 huDisCas7-11 S1006-GGGS-D1221 U6-NT guide

Plasmid

#186993

Purpose

AAV transgene plasmid for Cas7-11-S1006-GGGS-D1221, with U6 promoter-driven expression of non-targeting crRNA guide

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-s1006-gggs-d122, non-targeting crRNA guide

Use

AAV

Tags

Expression

Mutation

s1006-gggs-d1221 deletion

Promoter

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pDF0336 pAAV EFS-HvsCas7-11 for dual vector

Plasmid

#172515

Purpose

Encodes HvsCas7-11 mammalian expression casette in AAV backbone

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pAAV EFS-HvsCas7-11 for dual vector

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0582 huDisCas7-11 R1045-GGGS-R1122 U6-NT guide

Plasmid

#186991

Purpose

AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of non-targeting crRNA guide

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-r1045-gggs-r1122, non-targeting crRNA guide

Use

AAV

Tags

Expression

Mutation

r1045-gggs-r1122 deletion

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDF0044 CjcCas7-11 full locus with synthetic array

Plasmid

#172502

Purpose

Encodes the CjcCas7-11 full locus for bacterial expression

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

CjcCas7-11 full locus with synthetic array

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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