Showing: 141 - 160 of 175 results
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Plasmid#41095DepositorInsertLoquacious-PD (loqs Fly)
UseTags6xHis-HRV3CsiteExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
49535-sgFMR1_E17A
Plasmid#157782PurposeExpresses a sgRNA targeting stop codon of human FMR1 geneDepositorInsertFMR1 (FMR1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
49535-sgFMR1_E3B
Plasmid#157783PurposeExpresses a sgRNA targeting the 3rd exon of human FMR1 geneDepositorInsertFMR1 (FMR1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FMR1-E17C-3xFLAG
Plasmid#157784Purposedonor plasmid for C-terminal FLAG tag fusion of human FMR1DepositorInsertFMR1-FLAG (FMR1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pWL502-PAM
Plasmid#174382PurposePlasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterraneiDepositorInsertPAM-spacer
UseCRISPR; Archaeal expressionTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pWL502-crRNA
Plasmid#174383PurposeThe plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterraneiDepositorInserta crRNA targeting the template strand
UseCRISPR; Archaeal expressionTagsExpressionBacterialMutationPromoterPhaR promoterAvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_MTX-LUCID
Plasmid#119047PurposePlasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_MTX-LUCID_part1
Fab_MTX-LUCID_part2
UseTagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianMutationPromoterEF-1α + CMV/SV40 enhancers and IRESAvailabilityAcademic Institutions and Nonprofits only -
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FMR1-E17B-P2A-NLuc
Plasmid#157857Purposedonor plasmid for FMR1-NlucDepositorInsertFMR1-Nlu (FMR1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + Fla
Plasmid#84866PurposeBaculovirus Expression of LFn -Fla fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
Fla
UseBaculovirusTagsExpressionInsectMutationPromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + FlaAAA
Plasmid#84867PurposeBaculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.DepositorInsertsLFn
FlaAAA
UseBaculovirusTags6xHisExpressionInsectMutation3 C-terminal leucines have been replaced by alani…PromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + Needle
Plasmid#84868PurposeBaculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
Needle
UseBaculovirusTags6xHisExpressionInsectMutationPromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + PrgJ
Plasmid#84869PurposeBaculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
PrgJ
UseBaculovirusTags6xHisExpressionInsectMutationPromoterPolyhedrinAvailabilityAcademic Institutions and Nonprofits only -
pET15b LFn-Fla 3A
Plasmid#84872PurposeBacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.DepositorInsertsLFn
Fla
UseTagsHisExpressionBacterialMutation3 C-terminal leucines have been replaced by alani…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHAhFMRPiso1
Plasmid#48690PurposeExpresses hFMRP iso 1DepositorInsertFlagHA FMR1 Iso 1 (FMR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMV, 2x TetAvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHAhFMRPiso1I304N
Plasmid#48692PurposeExpresses hFMRP iso 1 I304NDepositorInsertFlagHA FMR1 Iso 1 I304N (FMR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMV, 2x TetAvailabilityAcademic Institutions and Nonprofits only -
TFORF1110
Plasmid#143763PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertISX (ISX Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pUC19-B9M9X8
Plasmid#103072PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertB9M9X8(Cas9 coding gene from Acidovorax ebreus (strain TPSY))
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailabilityAcademic Institutions and Nonprofits only -
pUC19-K2FTY8
Plasmid#103079PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorInsertK2FTY8(Cas9 coding gene from Alcanivorax pacificus W11-5)
UseCRISPR; Cloning vectorTagsExpressionMutationhuman codon-optimizedPromoterAvailabilityAcademic Institutions and Nonprofits only -
Showing: 141 - 160 of 175 results