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pHH0103_STAC3-2/2

Plasmid

#91466

Purpose

Protein expression and purification of human SH3 domain construct STAC3-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

STAC3-2/2 (STAC3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_STAC3-1/2

Plasmid

#91271

Purpose

Protein expression and purification of human SH3 domain construct STAC3-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

STAC3-1/2 (STAC3 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_STAC-1/2

Plasmid

#91527

Purpose

Protein expression and purification of human SH3 domain construct STAC-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

STAC-1/2 (STAC Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_STAC-2/2

Plasmid

#91302

Purpose

Protein expression and purification of human SH3 domain construct STAC-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

STAC-2/2 (STAC Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_STAC2-1/1

Plasmid

#91287

Purpose

Protein expression and purification of human SH3 domain construct STAC2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

STAC2-1/1 (STAC2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pNOC-stacked-aequorin

Plasmid

#101010

Purpose

Construct for expression of aequorin, calcium sensitive luciferase reporter and hygromycin resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Promoter

Ribi

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTH732-2µ-RLuc/staCFLuc

Plasmid

#40607

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

The last three codons of the full-length Firefly …

Promoter

ADH1 and TDH3

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pNOC-stacked-GeNL

Plasmid

#101002

Purpose

Construct for fusion expression of GeNL reporter and neomycin/G418 resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Promoter

Ribi

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pNOC-stacked-GeNL-Ca

Plasmid

#101003

Purpose

Construct for fusion expression of GeNL calcium reporter and neomycin/G418 resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis expression

Available Since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pTH727-CEN-RLuc/staCFLuc

Plasmid

#38211

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

The last three codons of the full-length Firefly …

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pNOC-stacked-GFP-aequorin

Plasmid

#101011

Purpose

Construct for expression of a calcium sensitive luciferase reporter, GFP-aequorin, and hygromycin resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Promoter

Ribi

Available Since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTH743-CEN-RLuc/slowstaCFLuc

Plasmid

#38223

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Inserts

Firefly Luciferase
Renilla luciferase

Expression

Yeast

Mutation

The last three codons of the full-length Firefly …

Promoter

ADH1 and TDH3 (=GPD)

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pTH825-CEN-minRLuc/slowstaCFLuc

Plasmid

#115370

Purpose

Expresses translation elongation-controlled Renilla luciferase and translation initiation-controlled firefly luciferase

Depositor

Tobias von der Haar

Inserts

Gcn4 5'-UTR + Firefly Luciferase
Renilla luciferase (codon minimised)

Expression

Yeast

Mutation

All codons have been changed for the least favour…

Promoter

ADH1 and TDH3

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pNOC-ARS-stacked-NeoR-mNeonGreen

Plasmid

#101249

Purpose

Construct for fusion expression of mNeonGreen reporter and G418 resistance gene by a bidirectional promoter

Depositor

Eva Farre

Article

Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

Type

Empty backbone

Use

Algae, nannochloropsis

Promoter

Ribi

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL3184

Plasmid

#41358

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-stac-Left (LOC100005402 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only
TAL3185

Plasmid

#41359

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-stac-Right (LOC100005402 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only
pRSET his-eGFP

Plasmid

#113551

Purpose

For bacterial expression and purification of eGFP

Depositor

Jeanne Stachowiak

Article

Busch et al Nat Commun. 2015 Jul 24;6:7875. doi: 10.

Insert

his-eGFP

Expression

Bacterial

Mutation

A206K

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET his-mCherry

Plasmid

#113552

Purpose

For bacterial expression and purification of mCherry

Depositor

Jeanne Stachowiak

Article

DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

Insert

his-mCherry

Expression

Bacterial

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET his-mRFP

Plasmid

#113553

Purpose

For bacterial expression and purification of mRFP

Depositor

Jeanne Stachowiak

Article

DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

Insert

his-mRFP

Expression

Bacterial

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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TfRΔEctoR1-mRFP-GFPnb

Plasmid

#113555

Purpose

Transmembrane model receptor to probe receptor internalization via clathrin mediated pathway

Depositor

Jeanne Stachowiak

Article

DeGroot et al Biophys J. 2018 Mar 27;114(6):1377-1388.

Insert

TfRΔEctoR1-mRFP-GFPnb

Expression

Mammalian

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge