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pRS403red-luxTA2-4F

Plasmid

#166030

Purpose

Expresses luxTA(2-4F) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

luxR

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

S116Y, W201R, codon-optimized for expression in y…

Promoter

pPGK1 mutant

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pGK415-luxTA2-4F

Plasmid

#165971

Purpose

Expresses luxTA(2-4F) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

luxR

Use

Synthetic Biology

Tags

Nuclear localization signal and three copies of V…

Expression

Yeast

Mutation

S116Y, W201R, codon-optimized for expression in y…

Promoter

pPGK1 mutant

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a-LuxT

Plasmid

#51183

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

LuxT

Use

Synthetic Biology

Tags

6x-his

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pETmsGFPSyt1(80 JuxtaK-142)

Plasmid

#209013

Purpose

Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Tags

6xHis-msfGFP

Expression

Bacterial

Mutation

aa 80-142 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pETSUMOSyt1C2AB(80 JuxtaK-421)

Plasmid

#209008

Purpose

Recombinant protein expression of truncated syt1 construct with Juxta K mutations

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pFSynWSyt1C2AB(80 JuxtaK-421)msGFP

Plasmid

#209016

Purpose

Overexpression of truncated syt1 with Juxta K mutations and tagged with GFP in mammalian cells

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Lentiviral

Tags

msfGFP

Expression

Mammalian

Mutation

aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pETSyt1C2AB(80 JuxtaK-421)msGFP

Plasmid

#209011

Purpose

Recombinant protein expression of truncated syt1 construct with Juxta K mutations and tagged with GFP

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Tags

6xHis and msfGFP

Expression

Bacterial

Mutation

aa 80-421 only, Juxta K mutant (80KKCLFKKKNKKKGKE…

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
L1-1147

Plasmid

#13267

Depositor

Vance Lemmon

Article

Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

Insert

L1CAM (L1CAM Human)

Use

Tags

Expression

Bacterial

Mutation

Truncated in the juxtamembrane region

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pAraGFP

Plasmid

#39548

Depositor

Bryan Berger

Article

Su et al J Biol Chem. 2012 Jul 22.

Insert

eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pAraTM

Plasmid

#39547

Depositor

Bryan Berger

Article

Su et al J Biol Chem. 2012 Jul 22.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pAraTM-2BTMCYwt

Plasmid

#39549

Depositor

Bryan Berger

Article

Su et al J Biol Chem. 2012 Jul 22.

Insert

Integrin alpha2B TM-CYTO

Use

Tags

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
pAraTM-2BTMCYL980A

Plasmid

#39550

Depositor

Bryan Berger

Article

Su et al J Biol Chem. 2012 Jul 22.

Insert

Integrin alpha2B L980A TM-CYTO

Use

Tags

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
pAraGFPCDF

Plasmid

#47516

Purpose

Contains PBAD::GFP reporter, used for heterodimer competition assay

Depositor

Bryan Berger

Article

Su et al J Mol Biol. 2013 Jul 19. pii: S0022-2836

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pAraTMwt

Plasmid

#47514

Purpose

Contains A2B L980A TMCY mutant, expresses wt AraC for heterodimer competition assay

Depositor

Bryan Berger

Article

Su et al J Mol Biol. 2013 Jul 19. pii: S0022-2836

Insert

Integrin alpha2B L980A TM-CYTO

Use

Tags

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAraTMDN

Plasmid

#47515

Purpose

Contains A2B L980A TMCY mutant, expresses DN AraC (AraC*) for heterodimer competition assay

Depositor

Bryan Berger

Article

Su et al J Mol Biol. 2013 Jul 19. pii: S0022-2836

Insert

Integrin alpha2B L980A TM-CYTO

Use

Tags

Expression

Bacterial

Mutation

Promoter

Trc

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pETSUMOSyt1C2AB(80-421)

Plasmid

#209005

Purpose

Recombinant protein expression of truncated syt1 construct

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

aa 80-421 only

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pETSyt1C2AB(80-421)msGFP

Plasmid

#209009

Purpose

Recombinant protein expression of truncated syt1 tagged with GFP

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Tags

6xHis and msfGFP

Expression

Bacterial

Mutation

aa 80-421 only

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pETSUMOSyt1C2AB(96-421)

Plasmid

#209006

Purpose

Recombinant protein expression of truncated syt1 construct

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

aa 96-421 only

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pETmsGFPSyt1(80-142)

Plasmid

#209012

Purpose

Recombinant protein expression of truncated syt1 tagged with GFP

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Tags

6xHis-msfGFP

Expression

Bacterial

Mutation

aa 80-142 only

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pETSUMOSyt1C2AB(143-421)

Plasmid

#209007

Purpose

Recombinant protein expression of truncated syt1 construct

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

aa 143-421 only

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge