We narrowed to 285 results for: Spr

#115927

~93,000 gRNAs targeting random positions along the genome of E. coli MG1655 with a NGG PAM motif. Contains ~19 targets per gene and can be used with SpdCas9 to perform pooled CRISPRi screens.

David Bikard

Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

June 6, 2019

Academic Institutions and Nonprofits only

#140633

Enables genome-wide CRISPR screening at high MOI through use of barcoded gRNA plasmids.

Wensheng Wei

Zhu et al Genome Biol. 2019 Jan 24;20(1):20. doi:

April 21, 2020

Academic Institutions and Nonprofits only

#247027

Human genome-wide CRISPR knockout library for the use of S. pyogenes Cas9. Each gene is targeted by 3 constructs.

John Doench, David Root

Drepanos et al bioRxiv 2025.08.26.672375; doi: https://

Oct. 2, 2025

Academic Institutions and Nonprofits only

#160134

Plasmid library harboring a randomized 10nt PAM 5’ of target site 3. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 5’ PAMs (e.g. Cas12a)

Keith Joung, Benjamin Kleinstiver

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Feb. 8, 2021

Academic Institutions and Nonprofits only

#160132

Plasmid library harboring a randomized 8nt PAM 3’ of target site 1. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

Keith Joung, Benjamin Kleinstiver

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Feb. 8, 2021

Academic Institutions and Nonprofits only

#160135

Plasmid library harboring a randomized 10nt PAM 5’ of target site 4. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 5’ PAMs (e.g. Cas12a)

Keith Joung, Benjamin Kleinstiver

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Feb. 8, 2021

Academic Institutions and Nonprofits only

#160133

Plasmid library harboring a randomized 8nt PAM 3’ of target site 2. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

Keith Joung, Benjamin Kleinstiver

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Feb. 8, 2021

Academic Institutions and Nonprofits only

#123361

CRISPR-Cpf1/Cas12a double knockout library. Targets 26 genes, chosen from analysis of human metastasis cohorts. It can be used to interrogate the genetic interactions driving metastasis.

Sidi Chen

Chow et al Nat Methods. 2019 May;16(5):405-408. doi

June 10, 2019

Academic Institutions and Nonprofits only

#209552

The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs.

John Doench

Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

Dec. 5, 2023

Academic Institutions and Nonprofits only

#209551

The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs.

John Doench

Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

Dec. 5, 2023

Academic Institutions and Nonprofits only