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We narrowed to 131 results for: Green fluorescence protein

Showing: 1 - 30 of 131 results

  1. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...Photoactivatable fluorescent proteins (PA-FPs) are fluorescent proteins that display unique changes in their spectral...et al. "EosFP, a fluorescent marker protein with UV-inducible green-to-red fluorescence conversion." Proceedings...Blog Read More about Fluorescent Protein Imaging Choosing Your Fluorescent Protein for Multi-Color Imaging...Fluorescent proteins (FPs) offer scientists a simple yet powerful way to tag cellular proteins and investigate...from low fluorescent states to high fluorescence states, they can change from one fluorescent color to...photoconvertible fluorescent proteins (PC-FPs). PC-FPs can convert from one fluorescent color to another...Selected Irreversible Photoactivatable Fluorescent Proteins Protein Oligomeric State Activating Light ...

  2. General Transfection

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    Protocol
    ...be seen here by the amount of green fluorescent protein expression (green in the right panels) with a limited...line and can be useful for short term studies of protein function. We specifically use this protocol with...with fresh media Day 3 or more (am): Observe fluorescence, harvest cells, or perform your experiment Equipment...batch of PEI is prepared, transfect cells with a fluorescent plasmid using a variety of ratios. Check the ...Incubate the cells 24–48 h before checking for protein expression. Sample Data Legend: Lenti-X 293T cells...

  3. Fluorescent Proteins 101: History of Fluorescent Proteins

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    Blog Post
    ..., as well as fluorescent ones, like Green Fluorescent Protein (GFP). These molecules occur naturally in...reach. Nobel Prize for fluorescent proteins The importance of fluorescent proteins was recognized in 2008...learning more about fluorescent proteins, I invite you to download our Fluorescent Protein eBook and learn...new green glowing protein could be used to measure gene transcription, as well as to track protein localization...the light they emit. Proteins that give off light include chemiluminescent proteins, like luciferases, .... The discovery of GFP The story of how fluorescent proteins like GFP came to be used as research tools... a ring that glows with a faint green light. To investigate this green glimmer, Shimomura collected many...

  4. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    Blog Post
    ...Bioluminescence and fluorescence from proteins such as Green Fluorescent Protein (GFP) has likely existed...applications in the lab. Why Green Fluorescent Protein? GFP is a ~27 kDa protein consisting of 238 amino acids...study protein-protein interactions. In this case, two portions of EGFP are fused to the proteins of interest... in fluorescent microscopy. Read More: All Plasmids 101 Articles Addgene's Fluorescent Protein Guide... green fluorescent protein...acting as a biosensor or cell marker, studying protein-protein interactions, visualizing promoter activity... Aequorea victoria. It has a fluorescent emission wavelength in the green portion of the visible spectrum...

  5. Fluorescent Proteins 101: Fluorescent Protein Timers

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    Blog Post
    ... Choosing Your Fluorescent Protein Resources at Addgene.org Find Fluorescent Protein Timers Browse All... All Fluorescent Protein Empty Backbones Check Out Our Fluorescent Protein Guide ...understand how protein localization changes over time.Hallmarks of fluorescent protein timers When FP...whether as part of protein fusions or expressed independently) originally fluoresce green or blue and, when...expressing a single FP-timer protein or protein fusion. This one protein method simplifies cloning and...Even before fluorescent proteins (FPs) came into wide use, there were a variety of ways to monitor cell...hybridization experiments. In many cases fluorescent proteins have usurped old methods or complemented...

  6. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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    Blog Post
    ...yellow-green fluorescent protein mNeonGreen is a yellow-green fluorescent protein that was derived from...either with standard green fluorescent protein filters or yellow fluorescent protein filters, with no or...about these proteins is that they are monomeric fluorescent proteins. Fluorescent proteins that oligomerize...plasmids containing the fluorescent protein mNeonGreen. This fluorescent protein joins mTFP1 and mWasabi... Read our fluorescent protein blog posts Find blog posts about choosing fluorescent proteins for your ...Michael Davidson fluorescent protein collection Check out Addgene’s fluorescent protein plasmids and resources...termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent protein at the ...

  7. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

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    Protocol
    ...transfection protocol Fluorescence Titering Assay for Lentivirus Quantify virus using fluorescence measurements...Purification Purify recombinant antibodies using Protein A or Protein G columns Watch the Video! Coomassie Purity...Watch the Video! AAV Titration by qPCR Using SYBR Green Technology Use qPCR to measure the physical titer...Video! Western Blot Separate and detect specific proteins Watch the Video! Immunocytochemistry Use antibodies...

  8. Fluorescent Protein Guide: Biosensors

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    Collection
    ...Plasmid Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal ...Glucose Green fluorescent glucose indicators for living cells (Glifons) Green Fluorescent Protein-Based ...Lactate Lindoblum green fluorescent biosensor for lactate Green fluorescent protein-based lactate and ...Pyruvate Pegassos green fluorescent biosensor for pyruvate Green fluorescent protein-based lactate and...cyclic AMP) Red fluorescent protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based cAMP... AAV Biosensors Fluorescent biosensors are proteins designed so that their fluorescent properties are ... from a nested cassette of green- and orange-emitting fluorescent proteins. Nat Commun. 2017 Sep 5;8(1...

  9. Which Fluorescent Protein Should I Use?

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    Blog Post
    ...categories of fluorescent proteins Photoactivatable / Photoconvertible: These proteins can switch their...state of the protein is non-fluorescent, thus allowing very low background level of fluorescence. Examples... photoconvertible proteins are PA-GFP, Dendra2, and the mEOS proteins. Some proteins are reversibly switchable...you, "Which fluorescent protein should I use?" And if you're looking for more fluorescence microscopy ...Interactive Visualization of Fluorescent Protein Properties  Fluorescence Spectrum Viewer  from BD bioscience...sheets on many FP variants Plasmids 101: Green Fluorescent Protein (GFP) Three Review Articles on the Different...processes” Curr. Protein. Pept. Sci. 9(4):338. Chudakov et. al. (2010) “Fluorescent proteins and their applications...

  10. Michael Davidson Fluorescent Protein Collection

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    Collection
    ...Michael Davidson Fluorescent Protein Collection Michael Davidson Fluorescent Protein Collection More Fluorescent...lab fluorescent protein fusion constructs and backbones. Plasmid... Plasmid Collections Fluorescent Proteins Michael...collection of ORFs tagged with a variety of fluorescent proteins. The majority of the ORFs are of mammalian...empty backbone with different fluorescent tags for you to create fusion proteins with your gene of interest...Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics The lab of Michael...Empty Backbones mEmeralds Reds Blues Cyans Oranges Greens Yellows Photoactivatables Empty Backbones Plasmid...

  11. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    Blog Post
    ...Light Sheet Fluorescence Microscopy Additional Resources on Addgene.org Fluorescent Protein Plasmids and... and Resources Fluorescent Protein Guide: Biosensors Keima, another fluorescent pH-biosensor for studying...supernatant (this is indicative of protein degradation). To control for protein degradation due to other pathways...an active form of the protein. To monitor autophagy, the Pho8Δ60 mutant protein is expressed. Pho8Δ60 ...Australian parrot. It’s comprised of two tandem fluorescent proteins: a relatively pH-insensitive RFP variant... is the permanent fluorescent tag portion of Rosella: it will emit red fluorescence regardless of its ...cell. SEP’s pH sensitivity means Rosella will fluoresce green unless it’s in an acidic environment like the...

  12. Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System

    Type
    Blog Post
    ...inoculation, no more fluorescent proteins are made. As the bacterial replicate, fluorescent proteins will be diluted...use a suite of fluorescent reporters. Bacteria containing unstable fluorescent proteins under the control...and Roger Tsien played enormous roles in fluorescent protein development by openly sharing their resources...conference of their choice to share their fluorescent protein based research. This year, we put out another...excited to see the many creative uses of fluorescent proteins. We’re happy to announce the three winners...approximately 75% of all protein-coding pathogenic human variants are decreased in protein abundance, possibly... reduced eGFP fluorescence. Cells expressing the library are then sorted by fluorescence-activated cell...

  13. Seeing Red: Simple GFP Photoconversion

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    Blog Post
    ...Addgene’s Fluorescent Protein Plasmids and Resources Relevant Blog Posts Plasmids 101: Green Fluorescent Protein...localization studies or make a reporter construct. Fluorescent proteins (FPs) are also the foundation of multiple... biological research. Photoconvertible fluorescent proteins Newly developed photoconvertible FPs like...allow one to monitor compartment/protein dynamics or evaluate protein turnover rate. In many cases, however... al., as well as a wide variety of other fluorescent proteins for your imaging needs. If you need an FP... WR, Menassa R, Hanson MR. Green to red photoconversion of GFP for protein tracking in vivo. Sci Rep. ... bright and photostable photoconvertible fluorescent protein. Nat Methods. 2009 Feb 6(2):131-3.  PubMed...

  14. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    Blog Post
    ...McNaughton mNeonGreen2 mNG2(1-10) and mNG2(11) Improved split fluorescent proteins for endogenous protein labeling...available to assess protein-protein interactions. One popular approach is to fuse a protein of interest to ... a split fluorescent protein (FP) and measure the signal produced when the candidate proteins’ interaction...assemble the full structure of the fluorescent protein, producing fluorescent signal. Image modified from Feng...Multiplexed labeling of cellular proteins with split fluorescent protein tags. Tamura R, Jiang F, Xie J,...Identification of new fluorescent protein fragments for bimolecular fluorescence complementation analysis...

  15. Choosing Your Fluorescent Proteins for Multi-Color Imaging

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    Blog Post
    ... of fluorescent proteins requires some thought. The first step in choosing fluorescent proteins for ... be aware of what fluorescent proteins are available. With new fluorescent proteins being published every... Choosing compatible fluorescent proteins To choose a set of fluorescent proteins to be imaged together...crosstalk between the two fluorescent proteins. If you must image fluorescent proteins whose spectra overlap...infrared fluorescent protein such as iFP1.4 or iFP2.0. Beware that these infrared fluorescent proteins require...with fluorescent proteins. With the right microscope and the right choice of fluorescent proteins, imaging...Clover is probably best; mNeonGreen is an even newer green fluorescent protein that is supposed to be extremely...

  16. CRISPRainbow and Genome Visualization

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    Blog Post
    ...about Fluorescent Protein-Based Tools Learn Some of the Pitfalls When Using Fluorescent Proteins Control...Control Protein Activity with GFP Resources on Asddgene.org Find Fluorescent Protein Plasmids Find Fluorescent...Fluorescent Protein Kits Find Empty Backbones with Fluorescent Protein Tags ...vivo. Some researchers have used fluorescently tagged DNA-binding proteins to label certain loci, but this...represents the composite of fluorescent measurements from red, blue, and green channels overlaid on a bright-field.... Each ortholog was fused to a different fluorescent protein to create three distinct colors. The specificity...containing two hairpin sequences, which recruit fluorescent proteins BFP, GFP, and RFP fused to the respective...

  17. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ...example green and red fluorescent proteins) allow simultaneous identification of more than one protein of ... found that fluorescent proteins might not always be the best option. Fluorescent proteins can interfere... in the expression of the protein of interest. The green fluorescent protein (GFP) and mCherry, two of...that non-fluorescent tags may be advantageous over fluorescent proteins when the fluorescence is not essential...fused to ‘tag’ or ‘label’ proteins to facilitate its detection. Fluorescent proteins are most widely used ...interfere with fused protein function Under some circumstances, the fused fluorescent proteins can be a roadblock...trafficking of the fusion protein (Kimple et al., 2013). We found that the fluorescent proteins can, indeed, interfere...

  18. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...circularly permuted green fluorescent protein (GFP), and nLightR uses a red fluorescent protein, mApple. Kagiampaki...findings that red fluorescent proteins tend to be less sensitive than green fluorescent proteins. nLightR did...the Addgene blog Fluorescent proteins 101: Fluorescent Biosensors Which Fluorescent Microscopy Technique...Plasmids Find Optogenetics Plasmids Visit the Fluorescent Protein Guide Pages ...sensitivity and speed and were only available as green probes. The Patriarchi Lab aimed to overcome these..., J., & Li, Y. (2019). A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection...S., & Steinegger, M. (2022). ColabFold: Making protein folding accessible to all. Nature Methods, 19(6...

  19. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...where an antibody binds a target protein in your sample, then a fluorescently labeled secondary antibody binds...anti-GABA-AR β3 (green). (C) Hippocampus labeled with anti-Kv2.1 (magenta), anti-AnkyrinG (green), and Hoechst...suitable antibody against your target protein, consider fusing a protein tag to the target and using an epitope-based...reagents like fluorescently-labeled nanobodies or scFvs. Many, many options for fluorescently conjugated ...palette for display (like green-magenta-blue or orange-blue rather than red-green). Even better, show each...Antibodies Which Fluorescence Microscopy Technique is Best for Me? Introduction to Immunofluorescence Resources...then you use a microscope to see where the target protein is (localization) and how much there is (quantification...

  20. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...indicators (GECIs) are fluorescent proteins linked to calcium binding proteins (e.g., calmodulin or troponin...CaMPARI. This sensor switches from green fluorescence to a red fluorescent state only by simultaneous (1) ...methods Many calcium reporters use a single fluorescent protein (FP), requiring only a simple measurement...Structure-and mechanism-guided design of single fluorescent protein-based biosensors. Nature chemical biology...Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’s Viral Services  ...troponin C). Their fluorescent readouts are used to monitor calcium concentrations and transients in living... of the sensor, and (3) subsequent change in fluorescence only occurs at high calcium concentrations. ...

  21. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    Blog Post
    ... Post on Protein Tags Read Other Posts on Fluorescent Proteins Browse Our Fluorescent Protein Pages Find...the untagged protein of interest. To avoid the accumulation of dark fluorescent proteins in your experiments...integral membrane proteins and membrane associated proteins (i.e. GPI-anchored proteins), which are more... JAIN, R., et al. "Oligomerization of green fluorescent protein in the secretory pathway of endocrine ...applicable to many microscopy experiments with fluorescent proteins (FPs) localized to compartments other than... cell physiology in general. Pitfalls of Fluorescent Protein Fusions Observing a bright signal for the... of the fusion protein molecules fail to correctly fold? Misfolded FPs do not fluoresce. A dark population...

  22. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ... the Fluorescent Protein Featured Topic Page Resources at Addgene.org Find Fluorescent Protein Biosensors...other Fluorescent Biosensors A Practical Approach to Choosing the B(right)est Fluorescent Protein Visit...periplasmic glutamate binding protein known as GltI. When the resulting fusion protein binds to glutamate, conformational...Biosensors Browse All Fluorescent Proteins Find AAV Tools References Marvin, Jonathan S., et al. “An optimized...conformational changes lead to an increase in fluorescence intensity from the inserted GFP. After characterizing...expression levels in bacteria and produces stronger fluorescent signals upon sensing glutamate in mouse and ferret... different colors – blue (SF-Azurite-iGluSnFR), green (SF-iGluSnFR), and yellow (SF-Venus-iGluSnFR). iGluSnFR3...

  23. Fluorescent Protein Guide: Empty Backbones

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    Collection
    ...Special Collections Fluorescent Protein Guide Empty Backbones Fluorescent Protein Guide: Empty Backbones...extensively by life scientists. The original green fluorescent protein (GFP) was cloned in 1992 ( Prasher et ...Expression mAzamiGreen 492 505 41 5.8 Monomer mAzami Green-N1 (mAG) - Mammalian Expression mAzami Green-C1 (... backbones with different fluorescent tags for you to create fusion proteins with your gene of interest...Backbones More Fluorescent Protein Resources: FRET Biosensors Subcellular localization Michael Davidson Collection... Imaging Blog: Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs Blog: When is a Monomer...Monomer not a Monomer? Background Fluorescent proteins are genetically encoded tools that are used extensively...

  24. Live and Let Dye: Self-Labeling Protein Tags

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    Blog Post
    ... — a green fluorescent protein, a red fluorescent protein, a fancy new green fluorescent protein, some...tags on different proteins of interest (or one self-labeling tag and a fluorescent protein) enables applications...new-and-improved fluorescent protein you want to try, just swap in a new fluorescent ligand and see the...plasmids containing the same protein of interest tagged with different fusion proteins for different experiments...tags, another version with that same red fluorescent protein somebody made because they forgot it was ...Just express the fusion protein and add a specific ligand! A self-labeling protein gives you the flexibility...chemical dyes provide many advantages over fluorescent proteins, opening up new applications.   Figure...

  25. Fluorescent Biosensors for Measuring Autophagic Flux

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    Blog Post
    ... to emission of only red fluorescence. The ratio of red to green fluorescence allows for clear distinction...mRFP-GFP-LC3 Tandem Fluorescent Protein, but following translation, this fusion protein is cleaved to generate...photoconversion Light sheet fluorescence microscopy Resources on Addgene.org Fluorescent protein plasmids and resources...biosensor that’s comprised of two tandem fluorescent proteins: a relatively pH-insensitive RFP variant...neutral pH cytoplasm where it emits red and green fluorescence. Following the induction of bulk autophagy...can also be fused to a protein of interest to track the autophagy of that protein. Find the cytosol-targeted... variant at Addgene! mRFP-GFP-LC3 tandem fluorescent protein This autophagy biosensor contains mRFP and...

  26. The Fluorescent Vegetables in Aptamer Soup

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    Blog Post
    ... selection of an RNA mimic of green fluorescent protein by fluorescence-based selection and directed evolution... applications of fluorescent RNA aptamers in Addgene’s collection. Green fluorescent aptamers Spinach ...activity of highly efficient RNA mimics of green fluorescent protein." Nature structural & molecular biology...small molecule activates the fluorescence of the small molecule. Fluorescent RNA aptamers are used to track...localization of the complex. Broccoli, the other green fluorescent aptamer, was identified through directed evolution... Resources at Addgene.org Browse fluorescent protein plasmids and resources See all Jaffrey lab plasmids...using aptamers might know this best, as most fluorescent aptamers are named for a fruit or vegetable! ...

  27. Download Science-Themed Zoom Backgrounds from Addgene

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    Blog Post
    ...interested in our fluorescent protein background. Download the orange fluorescent protein background.     ...not, you might want to check out Addgene’s Fluorescent Proteins blog topic to learn more about FRET and ...ready-to-use AAV and lentiviral preps! Download the green AAV background.       Some of our lentiviral plasmids...pooled barcoding libraries, and more. Download the green virus background.           Maybe your viral research...organism, research technique, and more. Download the green DNA background.     Since we ❤️ DNA at Addgene,...

  28. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...separate DNA or transforming cells with green fluorescent protein, these activities are limited to those...say green fluorescent protein – you first need the DNA sequence that encodes it. That DNA sequence then ...instructions it needs to produce that green fluorescent protein through transcription and translation....Making proteins outside of a cell - cell free extracts Typically, if you want to make a protein – say ... can easily sense, whether that’s bright fluorescent proteins that they can see, enzymes that can convert... live cells. The alternative is to express the protein in a cell-free manner. This is like taking an engine...systems contain all the parts needed to produce proteins without the need to handle a living, replicating...

  29. Bacterial Expression Systems

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    Collection
    ...Photoconvertible , and Photoswitchable fluorescent proteins Various PIs Fluorescent protein fusion: contains empty vectors...and C-terminal fluorescent protein tags that can be used to create fluorescent protein fusions with your...mEos2 20341 mEos2 (fluorescent protein) Loren Looger Photoactivable Fluorescent Protein Expression: contains...Empty Backbones with Fluorescent protein tags Various Plasmids Blue/UV , Cyan , Green , Yellow , Orange ...your protein of interest. See an overview of Addgene fluorescent protein resources here . pCyPet-His 14030...cyan fluorescent protein variant (CyPet) optimized for FRET measurements with a yellow fluorescent protein...yellow fluorescent protein variant (YPet) optimized for FRET measurements with a cyan fluorescent protein...

  30. Fluorescent Proteins 101: When GFP lets you down

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    Blog Post
    ...of the available green fluorescent proteins emit light. The pKa of fluorescent proteins can be accurately...use of bright red fluorescent proteins (e.g. mScarlet) or infrared fluorescent proteins (Chernov et al.,...Structural Dynamics of Green Fluorescent Protein Fused with a Single Chain Fv Protein." Journal of Biological...Introduction to GFP Read our Fluorescent Proteins Blog Posts Download the Fluorescent Proteins 101 eBook Resources... Browse the Fluorescent Protein Collection Find empty backbones for fluorescent protein fusions Browse...co-factor binding proteins, systems wherein a protein binds a synthetic, fluorescent analogue have been...for another protein. Effectively, it equips the protein of interest with an additional protein module that...
Showing: 1 - 30 of 131 results