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Showing: 1 - 40 of 56 results

  1. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...post). Optimizing FLP FRT through smart engineering Different FLP versions – making FLP efficient The first...fellow SSR system FLP-FRT. In addition, a variety of means to spatiotemporally control FLP and Cre expression...Read on to learn more about FLP-FRT, Cre-lox, and how combinations of FLP and Cre enable additional levels...levels of genetic control. What is FLP FRT and how does it work? FLP recombinase, which is named after ...Sadowski 1985). The FLP system works in a similar fashion to the Cre system. The FLP recombinase recognizes...(LDB) to the C-terminus of FLP or Cre (pCAG-CreERT2 #14797). These Cre/ FLP versions are retained in the...Andrea et al. 2010. “An Improved FLP Deleter Mouse in C57Bl/6 Based on FLPo Recombinase.” Genesis 48(8): ...

  2. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ... we are excited to add Flp-expression AAV to our recombinase AAV inventory. Flp is another useful recombinase...populations. We now offer a Flp-expressing AAV, as well as a Cre-dependent Flp-expressing AAV. See our recombinase...detection PSAM4 GlyR for chemogenetic inhibition Flp expression for intersectional studies And more! ...Serotype Name 55637  AAVrg pAAV-EF1a-Flpo 87306  AAV1 AAV pEF1a-DIO-FLPo-WPRE-hGHp Other new viral vectors...55634  AAV1  pAAV-EF1a-mCherry-IRES-Flpo 55637  AAV1  pAAV-EF1a-Flpo Chemogenetics Plasmid Serotype...

  3. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    ...with low cross-reactivity, as well as low toxicity. FLP/FRT recombinases (from Saccharomyces cerevisiae) ...with flies heterozygous for tubulin-GAL4. For Dre, Flp, KD, R, B2 and B3, approximately 50% of offspring...shows that the yeast recombinases KD, B2, B3 and FLP can be used together in Drosophila as a toolkit for...

  4. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...expressing both Cre and GFP. The GFP dependent FLPo, Flp-DOG (75469), is also available in AAV1. Plasmid...expression. Plasmid 75470 is now available in AAV1 for Flp-dependent expression of ChR2(H134R) bound to an mCherry...pAAV-EF1a-C-CreintG Connie Cepko 75469 AAV1 pAAV-EF1a-Flp-DOG-NW Connie Cepko Viral vectors coming soon...targeted expression DREADDs GFP dependent Cre and FLPo And more!  New lentiviral preps SARS-CoV-2 protein...

  5. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another site-directed... conceptually similar to Cre-lox, with flippase (Flp) and the short flippase recognition target (FRT) ...being analogous to Cre and loxP, respectively. The FLP-FRT technology can be an effective alternative to...

  6. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...of the site-specific recombinases (SSRs) Cre and FLP. SSRs bind DNA at target sites to induce site specific... events: Cre recombinase binds loxP sites, while FLP binds FRT sites. When a DNA sequence is flanked by...and a pair of FRT sites and include both Cre and FLP recombinases.   Figure 1 (modified from Schnutgen...

  7. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...transgenic models that express recombinases like Cre or Flp in their cells of interest and recombinase-dependent...sequences, lox (recognized by Cre) or frt (recognized by Flp), to either flip or excise a gene that is located...leaky expression in cells that do not express Cre or Flp. Here at Addgene we quantify the amount of transgene...expression can occur in cells that don’t express Cre or Flp, what can we do about it?  Test the serotype and ...

  8. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both Cre and Flp dependent. Plasmid Serotype Name 124603 AAV9...our calcium sensor collection to include our first Flp-dependent GCaMP, AAV8-105714. Several new options... Serotype Name 87306 AAVrg, AAV5 AAV pEF1a-DIO-FLPo-WPRE-hGHpA    Additional resources on the Addgene...

  9. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo (55634-AAV1) pAAV-EF1a-Flpo (55637-AAV1) Viral...collection. Of note, we are excited to release a Flp-dependent Cre AAV in AAV9 and AAVrg. See our new ...

  11. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...Specificity can be achieved using specialized promoters, Flp or Cre dependent gene switches, and serotype selection...cell-specific gene expression and viral infection. Cre or Flp dependent switches control gene expression via switches...

  12. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...including our first far-red fluorescent marker, a Flp-dependent channelrhodopsin in the retrograde serotype...

  13. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    .... It’s an alternative to the conventional Cre or Flp recombinases used to excise markers. It has the advantage...

  15. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...complementary Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP/FRT ...-responsive CreERT, the coupling of Cre-lox with FLP-FRT recombination to generate additional recombination... placed under different promoters, Brainbow and Flpbow can be used to label distinct cell populations....

  17. Deisseroth INTRSECT Collection

    Type
    Collection
    ...of populations expressing only Cre AND Flp, Cre NOT Flp, or Flp NOT Cre. B) This strategy has been expanded...Cre AND Flp, here expressed through a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp). Image...pAAV-hSyn Con/Fon EYFP Cre AND Flp Yes 231926 pAAV-Ef1a-Con/Fon-EYFP Cre AND Flp No 55651 pAAV-hSyn Con/Foff...Foff EYFP Cre AND NOT Flp F3/F5 No 137162 pAAV-Ef1a-Con/Foff 2.0-EYFP Cre AND NOT Flp FRT/F5 Yes 55652 pAAV-hSyn...pAAV-hSyn Coff/Fon EYFP Flp AND NOT Cre No 231927 pAAV-Ef1a-Coff/Fon-EYFP Flp AND NOT Cre Yes 137129 pAAV-Ef1a-Con...pAAV-Ef1a-Con/Fon-BFP Cre AND Flp Yes 137130 pAAV-Ef1a-Con/Foff 2.0-BFP Cre AND NOT Flp FRT/F5 Yes 137131 pAAV-Ef1a-Coff...-Ef1a-Coff/Fon-BFP Flp AND NOT Cre Yes 137132 pAAV-Ef1a-Con/Fon-mCherry Cre AND Flp Yes 137133 pAAV-Ef1a-Con...

  19. Control AAV Preps

    Type
    Collection
    ... tags Activity Cre-dependent Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive... mCherry Flp dependent 1, 2, 5, 8, 9, rg* Deisseroth 55641 pAAV-Ef1a-fDIO EYFP EF1a EYFP Flp dependent... EF1a tdTomato Flp dependent 1 Jensen 99133 pAAV-CAG-fDIO-mNeonGreen CAG mNeonGreen Flp dependent 1 Gradinaru...Fon-BFP EF1a BFP Flp dependent 8 Deisseroth 137134 pAAV-Ef1a-Coff/Fon-mCherry EF1a mCherry Flp dependent 8 ...EF1a oScarlet Flp dependent 8 Deisseroth 55650 pAAV-hSyn Con/Fon EYFP hSyn EYFP Cre and Flp dependent 8,... Cre and Flp dependent 8 Deisseroth 137132 pAAV-Ef1a-Con/Fon-mCherry EF1a mCherry Cre and Flp dependent...oScarlet Cre and Flp dependent 8 Deisseroth 137164 pAAV-nEF-Con/Fon/Von eYFP nEF EYFP Cre, Flp and VCre dependent...

  20. Optogenetics AAV Preps

    Type
    Collection
    ...fluorescent protein Activity Cre dependent Flp dependent Cre and Flp dependent Constitutive Serotype AAV1 AAV2...mCherry Flp dependent 1 Cepko 55639 pAAV-Ef1a-fDIO hChR2(H134R)-EYFP EF1a ChR2/H134R EYFP Flp dependent... Boyden 130909 AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] CAG ChrimsonR tdTomato Flp dependent 8 Boyden 100049...pAAV-nEF-Con/Fon-ChR2-mCherry nEF ChR2 mCherry Cre and Flp dependent 8 Deisseroth 137143 pAAV-nEF-Con/Foff 2.0... pAAV-nEF-Coff/Fon-ChR2-mCherry nEF ChR2 mCherry Flp dependent 8 Deisseroth 137163 pAAV-nEF-Con/Foff 2.0...Fon hChR2(H134R)-EYFP Syn ChR2/H134R EYFP Cre and Flp dependent 5, 8, 9, rg* Deisseroth 58880 pAAV-Syn-...Fon-ChR2(ET/TC)-EYFP nEF ChR2(E123T/T159C) EYFP Cre and Flp dependent 8 Deisseroth 137140 pAAV-nEF-Con/Foff 2.0...

  25. Retrograde AAV viral preps

    Type
    Collection
    ...Inhibitor, Flp-dependent DREADD Gether 154868 pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA Syn Activator, Flp-dependent...Controls Green Red Switch Other Recombinases Cre Flp VCre Dre Calcium Sensors GCaMP8f GCaMP8s GCaMP8m ...Roth 55650 pAAV-hSyn Con/Fon EYFP Syn EYFP, Cre and Flp-dependent Control Deisseroth 28306 pAAV-FLEX-tdTomato... Deisseroth 55641 pAAV-Ef1a-fDIO EYFP EF1a EYFP, Flp-dependent Control Deisseroth 114470 pAAV-Ef1a-mCherry...Deisseroth 114471 pAAV-Ef1a-fDIO mCherry EF1a mCherry, Flp-dependent Control Deisseroth 99130 pAAV-mDlx-NLS-... 137164 pAAV-nEF-Con/Fon/Von eYFP nEF EYFP, Cre, Flp and VCre-dependent Control Deisseroth 117382 hSyn1...Zhang 121675 pAAV-EF1a-fDIO-Cre EF1a Flp-dependent Cre recombinase Recombinases Engel , Nectow 50363 AAV phSyn1...

  26. Biosensor AAV Preps

    Type
    Collection
    ...element Activity Cre-dependent Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive...none Flp dependent 8 Deisseroth 137165 pAAV-Ef1a-Con/Fon/Von GCaMP 6m EF1a GCaMP6m none Cre, Flp and VCre... 128315 pAAV-Ef1a-fDIO-GCaMP6f EF1a GCaMP6f none Flp dependent 1 Jensen 135632 pAAV-S5E2-GCaMP6f E2 GCaMP6f...pAAV-Ef1a-Con/Fon-GCaMP6F EF1a GCaMP6f none Cre and Flp dependent 5, 8 Deisseroth 137123 pAAV-Ef1a-Con/Foff...137124 pAAV-Ef1a-Coff/Fon-GCaMP6F EF1a GCaMP6f none Flp dependent 8 Deisseroth 158757 AAV-CAG-SomaGCaMP6f2...pAAV-EF1a-Con/Fon-GCaMP6M EF1a GCaMP6m none Cre and Flp dependent 8 Deisseroth 137120 pAAV-Ef1a-Con/Foff ... 105714 pAAV-Ef1a-fDIO-GCaMP6s EF1a GCaMP6s none Flp dependent 8 Larsen 105715 pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s...

  27. Chemogenetics AAV Preps

    Type
    Collection
    ...mCitrine EGFP dTomato Activity Cre-dependent Flp-dependent Cre and Flp-dependent Constitutive (non-cre dependent...mCherry-WPREpA hM4D(Gi) - Inhibition mCherry fusion Flp-dependent 8, rg* Gether 154868 pAAV-hSyn-fDIO-hM3D...mCherry-WPREpA hM3D(Gq) - Activation mCherry fusion Flp-dependent 8, rg* Gether 119741 AAV SYN flex PSAM4...Gi-mCherry rM4D(Gi) - Inhibition mCherry Cre and Flp-dependent 8 Deisseroth 177673 pAAV-nEF-Con/Foff DREADD...

  28. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...EF1 promoter when FLP is expressed by another vector pCDH-EF1-FLPe 72262 Expresses FLPe from the EF-1 promoter...infectious virus, plasmids expressing both the Cre and Flp recombinases to remove genes flanked by either loxP...72260 Expresses copGFP from the EF1 promoter when FLP is expressed by another vector pCDH-EF1-Fon-tdTomato...expressing a variety of reporters as well as the Cre and Flp recombinases. Genes can be cloned into these transfer...from the CB promoter pCDH-CB-FLPe-P2A-copGFP-T2A-Puro 72258 Expresses FLPe, copGFP and the puromycin resistance...resistance from the CB promoter pCDH-CB-FLPe-P2A-tdTomato 72259 Expresses FLPe and tdTomato from the CB promoter...tdTomato from the CMV promoter pAdxEF1-FLPe-tdTomato 73352 Expresses FLPe and tdTomato from the EF1 promoter...

  30. Worm Expression Resources

    Type
    Collection
    ...diminishes epigenetic silencing in the germline. A "FLP-Out" system for controlled gene expression in Caenorhabditis...expression in C. elegans by combining expression of the FLP recombinase from either a heat-shock promoter or ...tissue-specific promoter and expression of the target FLP-out cassette from either a ubiquitous or a tissue-specific...

  32. Rabies and Neuronal Tracing

    Type
    Blog Post
    ... to the CNS and PNS Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist's View Control Gene Expression...

  33. AAV Molecular Tools

    Type
    Collection
    ...pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato EF1a-driven, Flp-dependent Flp-dependent expression of the voltage-gated ...

  35. CRISPR Plasmids - Tagging

    Type
    Collection
    .... An improved FLP recombinase expression vector allows for efficient marker cassette FLP-out. Plasmids...assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and...

  38. AAV Viral Preps

    Type
    Collection
    ...Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre Biosensors Calcium Sensors, Glutamate Sensors...

  40. Recombinases AAV Preps

    Type
    Collection
    ...rg* Zhang 75469 pAAV-EF1a-Flp-DOG-NW EF1a none 1 Cepko 183412 pAAV-CAG-FlpO CAG none 1, rg* Janelia Light-Inducible...5, rg* Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV phSyn1(S)-FlpO-bGHpA Syn none...Zeng 174378 pAAV-syn-Flpo Syn none rg* Wickersham 55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a mCherry (not a...Deisseroth 55637 pAAV-EF1a-Flpo EF1a none 1, 2, rg* Deisseroth 87306 AAV pEF1a-DIO-FLPo-WPRE-hGHpA EF1a none... Packaging Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible Recombinases VCre AAV Browse...-iDreV EF1a none 1, PHPeB Zeng 140137 pAAV-EF1a-iFlpV EF1a none 1, PHPeB Zeng VCre AAV ID Name Promoter...
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