Skip to main content
Addgene
Showing: 1 - 20 of 21 results

  1. Deisseroth INTRSECT Collection

    Type
    Collection
    ...of populations expressing only Cre AND Flp, Cre NOT Flp, or Flp NOT Cre. B) This strategy has been expanded...Cre AND Flp, here expressed through a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp). Image...pAAV-hSyn Con/Fon EYFP Cre AND Flp Yes 231926 pAAV-Ef1a-Con/Fon-EYFP Cre AND Flp No 55651 pAAV-hSyn Con/Foff...Foff EYFP Cre AND NOT Flp F3/F5 No 137162 pAAV-Ef1a-Con/Foff 2.0-EYFP Cre AND NOT Flp FRT/F5 Yes 55652 pAAV-hSyn...pAAV-hSyn Coff/Fon EYFP Flp AND NOT Cre No 231927 pAAV-Ef1a-Coff/Fon-EYFP Flp AND NOT Cre Yes 137129 pAAV-Ef1a-Con...pAAV-Ef1a-Con/Fon-BFP Cre AND Flp Yes 137130 pAAV-Ef1a-Con/Foff 2.0-BFP Cre AND NOT Flp FRT/F5 Yes 137131 pAAV-Ef1a-Coff...-Ef1a-Coff/Fon-BFP Flp AND NOT Cre Yes 137132 pAAV-Ef1a-Con/Fon-mCherry Cre AND Flp Yes 137133 pAAV-Ef1a-Con...

  2. Control AAV Preps

    Type
    Collection
    ... tags Activity Cre-dependent Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive... mCherry Flp dependent 1, 2, 5, 8, 9, rg* Deisseroth 55641 pAAV-Ef1a-fDIO EYFP EF1a EYFP Flp dependent... EF1a tdTomato Flp dependent 1 Jensen 99133 pAAV-CAG-fDIO-mNeonGreen CAG mNeonGreen Flp dependent 1 Gradinaru...Fon-BFP EF1a BFP Flp dependent 8 Deisseroth 137134 pAAV-Ef1a-Coff/Fon-mCherry EF1a mCherry Flp dependent 8 ...EF1a oScarlet Flp dependent 8 Deisseroth 55650 pAAV-hSyn Con/Fon EYFP hSyn EYFP Cre and Flp dependent 8,... Cre and Flp dependent 8 Deisseroth 137132 pAAV-Ef1a-Con/Fon-mCherry EF1a mCherry Cre and Flp dependent...oScarlet Cre and Flp dependent 8 Deisseroth 137164 pAAV-nEF-Con/Fon/Von eYFP nEF EYFP Cre, Flp and VCre dependent...

  3. Optogenetics AAV Preps

    Type
    Collection
    ...fluorescent protein Activity Cre dependent Flp dependent Cre and Flp dependent Constitutive Serotype AAV1 AAV2...mCherry Flp dependent 1 Cepko 55639 pAAV-Ef1a-fDIO hChR2(H134R)-EYFP EF1a ChR2/H134R EYFP Flp dependent... Boyden 130909 AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] CAG ChrimsonR tdTomato Flp dependent 8 Boyden 100049...pAAV-nEF-Con/Fon-ChR2-mCherry nEF ChR2 mCherry Cre and Flp dependent 8 Deisseroth 137143 pAAV-nEF-Con/Foff 2.0... pAAV-nEF-Coff/Fon-ChR2-mCherry nEF ChR2 mCherry Flp dependent 8 Deisseroth 137163 pAAV-nEF-Con/Foff 2.0...Fon hChR2(H134R)-EYFP Syn ChR2/H134R EYFP Cre and Flp dependent 5, 8, 9, rg* Deisseroth 58880 pAAV-Syn-...Fon-ChR2(ET/TC)-EYFP nEF ChR2(E123T/T159C) EYFP Cre and Flp dependent 8 Deisseroth 137140 pAAV-nEF-Con/Foff 2.0...

  4. Retrograde AAV viral preps

    Type
    Collection
    ...Inhibitor, Flp-dependent DREADD Gether 154868 pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA Syn Activator, Flp-dependent...Controls Green Red Switch Other Recombinases Cre Flp VCre Dre Calcium Sensors GCaMP8f GCaMP8s GCaMP8m ...Roth 55650 pAAV-hSyn Con/Fon EYFP Syn EYFP, Cre and Flp-dependent Control Deisseroth 28306 pAAV-FLEX-tdTomato... Deisseroth 55641 pAAV-Ef1a-fDIO EYFP EF1a EYFP, Flp-dependent Control Deisseroth 114470 pAAV-Ef1a-mCherry...Deisseroth 114471 pAAV-Ef1a-fDIO mCherry EF1a mCherry, Flp-dependent Control Deisseroth 99130 pAAV-mDlx-NLS-... 137164 pAAV-nEF-Con/Fon/Von eYFP nEF EYFP, Cre, Flp and VCre-dependent Control Deisseroth 117382 hSyn1...Zhang 121675 pAAV-EF1a-fDIO-Cre EF1a Flp-dependent Cre recombinase Recombinases Engel , Nectow 50363 AAV phSyn1...

  5. Biosensor AAV Preps

    Type
    Collection
    ...element Activity Cre-dependent Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive...none Flp dependent 8 Deisseroth 137165 pAAV-Ef1a-Con/Fon/Von GCaMP 6m EF1a GCaMP6m none Cre, Flp and VCre... 128315 pAAV-Ef1a-fDIO-GCaMP6f EF1a GCaMP6f none Flp dependent 1 Jensen 135632 pAAV-S5E2-GCaMP6f E2 GCaMP6f...pAAV-Ef1a-Con/Fon-GCaMP6F EF1a GCaMP6f none Cre and Flp dependent 5, 8 Deisseroth 137123 pAAV-Ef1a-Con/Foff...137124 pAAV-Ef1a-Coff/Fon-GCaMP6F EF1a GCaMP6f none Flp dependent 8 Deisseroth 158757 AAV-CAG-SomaGCaMP6f2...pAAV-EF1a-Con/Fon-GCaMP6M EF1a GCaMP6m none Cre and Flp dependent 8 Deisseroth 137120 pAAV-Ef1a-Con/Foff ... 105714 pAAV-Ef1a-fDIO-GCaMP6s EF1a GCaMP6s none Flp dependent 8 Larsen 105715 pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s...

  6. Chemogenetics AAV Preps

    Type
    Collection
    ...mCitrine EGFP dTomato Activity Cre-dependent Flp-dependent Cre and Flp-dependent Constitutive (non-cre dependent...mCherry-WPREpA hM4D(Gi) - Inhibition mCherry fusion Flp-dependent 8, rg* Gether 154868 pAAV-hSyn-fDIO-hM3D...mCherry-WPREpA hM3D(Gq) - Activation mCherry fusion Flp-dependent 8, rg* Gether 119741 AAV SYN flex PSAM4...Gi-mCherry rM4D(Gi) - Inhibition mCherry Cre and Flp-dependent 8 Deisseroth 177673 pAAV-nEF-Con/Foff DREADD...

  7. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...EF1 promoter when FLP is expressed by another vector pCDH-EF1-FLPe 72262 Expresses FLPe from the EF-1 promoter...infectious virus, plasmids expressing both the Cre and Flp recombinases to remove genes flanked by either loxP...72260 Expresses copGFP from the EF1 promoter when FLP is expressed by another vector pCDH-EF1-Fon-tdTomato...expressing a variety of reporters as well as the Cre and Flp recombinases. Genes can be cloned into these transfer...from the CB promoter pCDH-CB-FLPe-P2A-copGFP-T2A-Puro 72258 Expresses FLPe, copGFP and the puromycin resistance...resistance from the CB promoter pCDH-CB-FLPe-P2A-tdTomato 72259 Expresses FLPe and tdTomato from the CB promoter...tdTomato from the CMV promoter pAdxEF1-FLPe-tdTomato 73352 Expresses FLPe and tdTomato from the EF1 promoter...

  8. Worm Expression Resources

    Type
    Collection
    ...diminishes epigenetic silencing in the germline. A "FLP-Out" system for controlled gene expression in Caenorhabditis...expression in C. elegans by combining expression of the FLP recombinase from either a heat-shock promoter or ...tissue-specific promoter and expression of the target FLP-out cassette from either a ubiquitous or a tissue-specific...

  9. AAV Molecular Tools

    Type
    Collection
    ...pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato EF1a-driven, Flp-dependent Flp-dependent expression of the voltage-gated ...

  10. CRISPR Plasmids - Tagging

    Type
    Collection
    .... An improved FLP recombinase expression vector allows for efficient marker cassette FLP-out. Plasmids...assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and...

  11. AAV Viral Preps

    Type
    Collection
    ...Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre Biosensors Calcium Sensors, Glutamate Sensors...

  12. Recombinases AAV Preps

    Type
    Collection
    ...rg* Zhang 75469 pAAV-EF1a-Flp-DOG-NW EF1a none 1 Cepko 183412 pAAV-CAG-FlpO CAG none 1, rg* Janelia Light-Inducible...5, rg* Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV phSyn1(S)-FlpO-bGHpA Syn none...Zeng 174378 pAAV-syn-Flpo Syn none rg* Wickersham 55634 pAAV-EF1a-mCherry-IRES-Flpo EF1a mCherry (not a...Deisseroth 55637 pAAV-EF1a-Flpo EF1a none 1, 2, rg* Deisseroth 87306 AAV pEF1a-DIO-FLPo-WPRE-hGHpA EF1a none... Packaging Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible Recombinases VCre AAV Browse...-iDreV EF1a none 1, PHPeB Zeng 140137 pAAV-EF1a-iFlpV EF1a none 1, PHPeB Zeng VCre AAV ID Name Promoter...

  13. Cre-lox system

    Type
    Collection
    ...multiple tissues. FLP/FRT: Alternative recombinase system for the control of gene expression. Flp recombinase...orientation of the FRT sites. FlpO constructs Addgene Blog Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist...recombinase recognizes FRT sites. Flp binding to these sites can result in excision, inversion or translocation...129419 pCag FlpO-2A-Cre EV FlpO-2A-Cre CAG Mammalian Breunig 130986 pCag SE (Self-excising) FlpO-2A-Cre EV...pCAG-Cre Cre CAG Mammalian Chenn 26745 pDIRE iCre and FlpO, required for dual RMCE EF-1 alpha Mammalian Zeller... EV Self-excising FlpO-2A-Cre CAG Mammalian Breunig 131780 pCS2+PIF6CreN CreLite - Red light-inducible...

  14. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...expression of GCaMP6m/s in cells depdent on Cre, Flp, or Vcre Comprehensive Dual- and Triple-Feature Intersectional...viral expression of sRGECO (constitutive or Cre- or Flp-dependent) Comprehensive Dual- and Triple-Feature...

  15. Brain Initiative Collection

    Type
    Collection
    ...AAV1 pAAV-CAG-fDIO-mNeonGreen An AAV genome with Flp recombinase-dependent expression of mNeonGreen from...pAAV-EF1a-iFlpV can be used to generate AAV virus that will express light-inducible site-specific iFlpV recombinase...

  16. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Isocortex 230518 pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA AiP1211 AiE2051m FlpO Layer 6_IT_Car3 Isocortex 224000...Isocortex 222939 pAAV-DLX2.0-minBG-FlpO-WPRE-HGHpA AiP14532 DLX2.0 FlpO Pan GABAergic interneurons Isocortex...Isocortex 230607 pAAV-AiE2103m-minBG-FlpO-WPRE-HGHpA AiP1252 AiE2103m FlpO Lamp5 interneurons Isocortex 223971...subplate 230518 pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA AiP1211 AiE2051m FlpO Claustrum Cortical subplate 191720...214855 pAAV-AiE0779m_3xC2-minBG-FlpO-WPRE3-BGHpA AiP14201 AiE0779m_3xC2 FlpO DRD1 Medium spiny neurons (D1...Striatum 230871 pAAV-AiE0452h-minBG-FlpO-WPRE3-BGHpA AiP14573 AiE0452h FlpO DRD2 Medium spiny neurons (D2-MSN...3xSP10ins-AiE0354m-minRho-SYFP2-WPRE3-BGHpA AiP12040 AiE0354m FlpO Vip interneurons Isocortex 230101 pAAV-hsA2-AiE0219h-minRho-SYFP2...

  17. Caltech Systemic Capsids

    Type
    Collection
    ... iDreV Zeng 140137 pAAV-EF1a-iFlpV EF1a light-inducible recombinase iFlpV Zeng 164450 CN1851-rAAV-hI56i-minBglobin-iCre...

  18. AAVED

    Type
    Collection
    ...Breakfast and Welcome Melina Fan 9:30 AM Cre and Flpo Katherine Matho 10:15 AM AAV Retrograde Tracing ...
Showing: 1 - 20 of 21 results