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We narrowed to 1,006 results for: RING

Showing: 1 - 40 of 1006 results

  1. TALEN Engineering

    Type
    Collection
    ... Genome Engineering TALEN Kits TALengineering Reagents Joung Lab TAL Effector Engineering Reagents You... found on the TALengineering website. Users may also wish to join the Genome Engineering newsgroup. An...Reagents from the Keith Joung laboratory for engineering TAL effectors, including designed TALENs, and... Joung Lab has developed three platforms for engineering TAL effector repeat arrays of any desired length...

  2. CRISPR History and Development for Genome Engineering

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    Collection
    ...system is especially amenable to genome engineering, offering flexibility and easy multiplexing/scaling...genome engineering tool. CRISPR... CRISPR CRISPR History CRISPR History and Development for Genome Engineering You ...research applications, eclipsing past genome engineering technologies like zinc finger nucleases (ZFNs...CRISPR was the first system harnessed for genome engineering, with Type V following in 2015. Class 1 (Multi-subunit...-switches for CRISPR genomic editing. Genome Engineering Applications In 2012, Jinek et al. first demonstrated...editors converted cytidine to thymidine; newly engineering base editors convert adenosine to guanosine. ...

  3. p53 Pathway

    Type
    Collection
    ...Checkpoint kinase 1 CHK2 Checkpoint kinase 2 Cop-1 Ring finger and WD repeat domain 2 (RFWD2); E3 ubiquitin...domain protein 1 PIGs Etoposide induced 2.4 PIRH-2 Ring finger and CHY zinc finger domain containing 1 (...2014 May;14(5):359-70. PubMed PMID: 24739573 . Uncovering the role of p53 splice variants in human malignancy...gain-of-function in cancer. Oren, M. and V. Rotter. Cold Spring Harbor Perspectives in Biology. 2010 Feb;2(2):a001107...

  4. Sharing is Caring: But How to Distribute Open Hardware?

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    Blog Post
    ...sharable products. Open-Source Microscopy Sharing Hardware vs. Sharing Software In the field of microscopy,...relatively young movement of sharing open hardware has a similar goal. The sharing of modules, ideas, and blueprints...material (BOM) of an assembly, the feasibility of manufacturing, and subsequent assembly can be difficult to... or 3D printers are key to enabling this work. Sharing open-source plans that rely on such tools has allowed...3D printing, on-demand CNC milling, and PCB manufacturing. These newly developed techniques are the key...enablers for making assemblies open-source. The sharing of digital representations of the 3D models, which...especially with regard to licensing models. Direct sharing of work from universities and research centers ...

  5. Fostering communication in remote collaborations

    Type
    Blog Post
    ... too. Schedule regular meetings Scheduling a recurring meeting every two weeks or every month is long...  Maintain informal communication In between recurring meetings, you should share interesting results...they’re set by checking in about them at your recurring meetings and reaching out if any milestones are...

  6. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    ... efforts check out Leila Haery’s blog series, “Bringing Sustainable Practices to the Lab.”  Whether participating...newsletter Help other scientists save resources by sharing plasmids Find plasmids at Addgene ...

  7. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...worry a very little bit about hiring when we need to do it quickly. Hiring is hard but as a 3 year Boston... space here at our new location to grow. We are hiring again and getting ready to embark on new exciting...distributed in 2018 and we are poised for more offerings. The viral vector team is publishing research,...We must keep spreading the word about materials sharing, but we don’t have to worry about getting support...False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button=False, width='1280', height...

  8. Corralling and Cornering the Goldilocks Antibody

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    Blog Post
    ... relationship to signal depend on what you’re comparing your sample to. For example, we use our negative...screening and development of a focused reference bring us one step closer to resolving the antibody crisis...

  9. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...terminal repeats (LTR). The 3’ LTR is duplicated during lentiviral integration, producing a second copy...voltage fluctuations, while having high fidelity during action potentials of fast-spiking neurons. The ...baseline voltage), the investigators found that administering these AAVs with a dilute Cre-expressing vector...splitting a large gene into smaller fragments, delivering them by individual AAVs, and then splicing their...

  10. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ... to insert your genetic cargo of choice. After hearing about researchers' favorite features and limitations...important modulators of cell signaling pathways during embryonic development and cancer. But there is ...

  11. Twenty Years of Sharing Science!

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    Blog Post
    ...resources. We've got twenty years of experience we're sharing through blogs, eBooks, videos, guides, protocols...

  12. A History of Genome Engineering in Popular Culture

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    Blog Post
    ...imagination of most of the authors during this period.  Genetic Engineering Era: Pre-CRISPR  Having visualized...associated with genetic engineering in the recent SciFi movies. Rampage (2018), starring Dwayne “The Rock” ...California, San Diego. The revolution in genetic engineering techniques is a speculation of yesteryear which...but the common themes that these authors were exploring were space and robots. Warping to stars in space...devise methods to manipulate it. The term genetic engineering itself is attributed to SciFi writer Jack Williamson...s Island. In 1976, the world’s first genetic engineering company, Genentech, was founded.  This commercialization...these corporations would extrapolate genetic engineering to exploit human beings. Ridley Scott directed...

  13. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...circuit by integrating neuronal signalling and triggering release of the stress hormone norepinephrine ...Cre-expressing AAV-5 into the DRN of a transgenic mouse harbouring a Cre-dependent IL-1 beta overexpression cassette...lit (risky) and a dark (protected) area. When measuring the time spent in a particular part of the apparatus...levels within the hippocampus and frontal cortex during depression, yet there have also been contradictory...

  15. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...waste. This is the second in a series of posts on bringing sustainable practices in the lab. The first post...waste from the landfill, but what about all the manufacturing steps that happen before you even receive the... lower the environmental impact in terms of manufacturing, energy, and water use. ACT labels are obtained...newsletter Help other scientists save resources by sharing plasmids Find plasmids at Addgene ...

  16. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    ...articles in this series: Bringing Sustainable Practices to the Lab: Innovations Bringing Sustainable Practices...Labconscious. I was eager to learn about practices I could bring both to Addgene, and share with my peers. In fact...the rate at which that happens and reduce the manufacturing of new containers, which is associated with ...huge contributor to the increase in plastic manufacturing, accounting for 40% of total plastic usage (...to be sustainability leaders. Take initiative to bring ideas to your workplace and you could end up making...newsletter Help other scientists save resources by sharing plasmids Find plasmids at Addgene ...

  17. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...Yoshii, Saori R., and Noboru Mizushima. "Monitoring and measuring autophagy." International journal of molecular...mitochondria-targeting sequence makes Keima useful for monitoring mitophagy. It’s important to remember that because...3B, or LC3. LC3 is a commonly used marker for measuring autophagic flux. mRFP-GFP-LC3 functions as an ...Maria Cuervo, and Per O. Seglen. "Methods for monitoring autophagy from yeast to human." Autophagy 3.3...

  18. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...genome engineering have played a huge role. For instance, researchers are genetically altering tumor cells... works on genome engineering and cancer immunotherapy, and is interested in exploring new avenues in healthcare...ve been studying it and, now, researchers are exploring ways to bolster and modulate our immune defenses...environments for tumor cells spreading to other organs during metastasis. Evading Recognition Tumor cells over-express...cause a transient shutdown of the immune system, hampering its ability to inhibit further cancer growth and...increasing life expectancy by 4.1 months as opposed to curing the cancer. DCs are harvested from the body, and...trials going amiss and patient deaths have occurred during CAR-T cell tests because treatment regimens were...

  19. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...chromosomal DNA.  Plasmids are said to be under stringent control when they depend on the host cell’s initiation...consider the replication mechanism of plasmids under stringent control, such as pSC101. DNA replication and regulation...replication for the bacterial chromosome and for stringently controlled plasmids. Binding of IHF (green) redistributes... cell growth or even kill the host cell. For stringently controlled plasmids, replication is tightly coupled...number.  Let’s consider pSC101. This plasmid is stringently controlled and has a low copy number. Like the... necessary. Iterons are not the only tool to stringently regulate plasmid replication. For example, the...Comparison of replication regulation mechanisms of stringently controlled plasmids pSC101 and ColIb-P9. pSC101...

  20. Twenty Years of Addgene Sharing: CRISPR

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    Blog Post
    ...also a story of community, cooperation, and sharing. Sharing this story is a truly special way to celebrate...As we celebrate twenty years at Addgene, we are sharing stories of our repository’s positive impact on ... that developed CRISPR tools were committed to sharing them with the rest of the community. They did this...the usefulness of popular CRISPR tools is both enduring and rapidly recognized.   Where is the CRISPR ...appropriate MTAs and customs forms. Of course, ordering the plasmids is the easy part; using them can ... resources on the Addgene blog Twenty Years of Sharing Science  ...

  22. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...preparation steps of production, purification, and titering. Our newest viral vector service, AAV Packaged...

  23. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...Addgene blog Twenty Years of Addgene Sharing: CRISPRTwenty Years of Sharing: Addgene's Viral Vector Service...many countries, import and export regulations, differing customs processes, and the distances the packages...specific to that country. ‌For example, when we began offering temperature-sensitive materials, we noticed a ...shipments arrived in India at room temperature. Partnering with our distributor allowed us to ensure that...shipments weren’t able to clear import control. Partnering with a distributor allowed us to share materials...scientists in China. They were also able to help with sharing information through the use of popular Chinese ...solutions aren’t enough to ensure accessibility, partnering with distributors who have expertise in a specific...

  24. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...2012). This made them an appealing target for bioengineering, but it wasn’t clear whether they could 1) ...bodies We have shown that all three of these engineering objectives are possible in our recent paper (...from This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & Rosati, G. Defensive...

  25. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...cycle for titering as this will better represent future experiments. The aforementioned titering options...in the long run. Read on for an overview of the titering options as well as the benefits and drawbacks ...measurements via ELISA and qPCR for viral RNA. Measuring p24 levels in a viral prep is straightforward ...transfer plasmid was used in the transfection; if preparing several different lentiviruses in parallel, this...should be considered. Similar to the p24 assay, titering via measurement of viral RNA can overestimate ...viral particles. One of the si mplest methods of measuring functional titer uses FACS to count the number...antibody staining. Check Out Our In-Depth Fluoresence Titering Protocol Another popular approach utilizes the...

  26. pSiM24: Simplifying Plant Genetic Engineering

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    Blog Post
    ...of modified Ti plasmids during plant infection, as well as plasmid yield during preparation in E. coli....the plants that we grow and eat, and genetic engineering will continue to improve plant nutrition, yield..., and pest resistance. Much of plant genetic engineering revolves around Agrobacterium tumifaciens. Agrobacterium...Agrobacterium to ensure that they are maintained during co-cultivation with the target plant species. Thus...with biolistic methods, making it useful for engineering plants that are not susceptible to Agrobacterium...customizability, pSiM24 can be used not only for plant engineering and biotechnology applications, but also for ... plant genetic engineering...

  27. Sleeping Beauty Awakens for Genome Engineering

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    Blog Post
    ...these defenses. For some applications of genome engineering - such as certain forms of gene therapy - avoiding...tool and an alternative to CRISPRs for genome engineering whose use is only likely to grow in the coming...  genome engineering...

  28. Reagent Repositories Are Speeding up Science During the Pandemic

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    Blog Post
    ...-2, but with many academic research labs closed during the pandemic, it’s hard to get these reagents. ...available through a repository, they remain accessible during these times. Centralized reagent repositories, ... rapid access to validated reagents, especially during a time when these materials may not be available...for further investigation of this pathogen. The sharing and distribution of cell lines from repositories...community will continue having access to plasmids during the COVID-19 pandemic. Over 7,000 plasmids for ... Case study 3: Mouse strains Reagents developed during the SARS-CoV pandemic more than a decade ago are...et al., 2006). Now that a similar phenomena is appearing with regards to SARS-CoV-2, this mouse strain ...

  30. Scientific Sharing in the Time of COVID-19: Databases and Resources

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    Blog Post
    ...been able to progress so rapidly is scientific sharing. Already we’ve seen articles about COVID-19 on ...centralized databases and centers to support the sharing of materials and data associated with COVID-19 ... these resources and why they value scientific sharing as the scientific community strives to understand... motivations vary, everyone we spoke with sees sharing and collaboration as the best way that the scientific...are supporting their access to useful resources during this time. Facilitate collaborations within ...researcher studying COVID-19. “Open scientific sharing is important for creating research synergies and...iterating on methods for coronavirus research, sharing resources for COVID-19 research in their field ...

  32. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...stably integrates into the genome and is propagated during normal DNA replication and cell division. Mobile-CRISPRi... these plasmids for transfer to recipient cells during a tri-parental mating scheme. Mobile-CRISPRi integrates...was variable. They measured transfer rates by comparing the number of colonies that grew on a selective...about other CRISPR methods for bacterial genome engineering   Learn about human CRISPRko, CRISPRi, and CRISPRa...

  34. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...determine functional titer with a colony formation titering assay or similar protocol. After functional titer...skewed the representation of your pooled library during the amplification process. It’s far better to verify...worked.  Ready, optimize, transduce!  If you’re ordering a pre-made library, you don’t need to worry about...optimize before you can transduce your cells.  Titering After generating your lentivirus, you’ll want ...specifically to allow scientists to perform functional titering and optimize transduction efficiency. Transduction...page from our book and put aside an aliquot for titering and optimizing before you freeze down your viral...and validation of CRISPR libraries. The Genome Engineering using CRISPR/Cas Systems Forum- Moderated by ...

  36. Networking and Inspiring Young Scientists at Freshers Fairs

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    Blog Post
    ...for positions both inside and outside academia. Inspiring future scientists At Addgene, we’re incredibly... a scientist and what benefits did they provide during your education? Let us know in the comments section...

  37. Gaining Leadership Skills Volunteering at a Professional Organization

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    Blog Post
    ...experience working on and later chairing the Advocacy Committee. I’m now furthering my leadership experience ...abilities we often hear that scientists should acquire during their PhD and postdoctoral training. Addgene executive...more substantial experience can be achieved by volunteering for a professional organization or a scientific...Vision and Ophthalmology Research (ARVO). Hopefully sharing my experience will show you how you can get started...started on your own development as a leader. Chairing a committee I was Chair of the Advocacy Committee...updated some sections of the NPA’s website. By volunteering, you can gain experience working with other ... my verbal and written communication skills. Volunteering on these committees was real work; meetings ...

  38. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ... start a peer mentoring group? "What Makes a Good Mentor?" Form Your Own Peer Mentoring Group: A How-...presentation skills (as much as you can), form a peer mentoring group to get real feedback Write whenever you ...How-To Guide for Scientists Download our eBook: Mentoring for Scientists [PDF] ...

  39. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...about her mentoring style. Set goals with a mentor: The secret of accountability To make a mentoring relationship...want more great advice on mentoring, click below to download the "Mentoring for Scientists" ebook, which... the fifth and final post in the Addgene Blog Mentoring for Scientists Series. The entire series and additional... If you have been following the posts in this Mentoring for Scientists series, you have: realized the ...adding formality and active goal setting to your mentoring relationships, so that you can reap rewards in...Joanne's Reddit AMA Add a little formality to mentoring Just getting together and chatting with another...about their career path is a great start to a mentoring relationship, but if you don’t take this past ...

  40. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ...brain at a level of abstraction that enables the engineering of its function." Once scientists can do that...big questions that motivate his interest in neuroengineering, and some of the optogenetic tools that are...are still missing. Optogenetic tools for neuroengineering Addgene: For those who may not be experts, can...set of neurons, maybe we could treat patients suffering from Parkinson's, chronic pain, deafness, or other... neuroengineering...
Showing: 1 - 40 of 1006 results