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  1. Gibson Assembly Protocol

    Type
    Protocol
    ... Protocols Gibson Cloning Gibson Assembly Cloning You may also...to include in the Gibson reaction are different than with normal PCR primers (Gibson et al. , 2010). Number...new window) . Gibson Assembly® is licensed to New England Biolabs by TelesisBio. Gibson DG, Young L, Chuang...nmeth.1318 (Link opens in a new window) Gibson DG, Smith HO, Hutchison CA, Venter JC, Merryman C. (2010). ...Get started with Gibson Assembly Cloning! Protocols...Molecular Biology Protocols Summary In 2009 Dr. Daniel Gibson and colleagues at the J. Craig Venter Institute...easy assembly of multiple linear DNA fragments (Gibson et al. , 2009). Regardless of fragment length or...
  2. Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

    Type
    Protocol
    ...the proper personal protective equipment (PPE) for BSL-1 and BSL-2 labs.... Protocols Personal Protective...Protective Equipment (PPE) for BSL-1 and BSL-2 Labs Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs...
  3. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...from the Michael Davidson Lab.... Plasmid Collections Fluorescent Proteins Michael Davidson Fluorescent Protein...Protein Collection Michael Davidson Fluorescent Protein Collection Empty Backbones Blue Cyan Green mEmerald...Orange Red Photoactivatable The lab of Michael Davidson from Florida State University has contributed ...localization data found below. In addition, the Davidson lab has contributed the corresponding empty backbone...post about this collection , or visit Michael Davidson's Molecular Expressions website (Link opens in ...
  4. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Nucleolus (fibrillar center) 133964 SON-mEGFP AICSDP-82 mEGFP SON Nuclear speckles 159744 DMD-mEGFP AICSDP...A., Grancharova, T., Arakaki, J., Fuqua, M. A., Nelson, A., Hookway, C., Ludmann, S. A., Mueller, I. A...
  5. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...eukaryotic genomes.  Components of a transposon system Transposon: The transposon is the DNA sequence that moves...also called retrotransposons, and Class II TEs, also called DNA transposons. DNA transposons are commonly...discovered transposons due to changes in coloration of corn kernels. Aside from corn, transposons are in ...2010).  So why do transposons matter? The outcome really depends on where a transposon lands. For example...components of transposons, how they are classified, and how they are used in the lab. What is a transposon? DNA...Transposable elements (TEs): Retrotransposons  Class I TEs are also known as retrotransposons. They transpose by...which are required for LTR retrotransposon transposition. Non-LTR retrotransposons contain two open reading...
  6. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...tests. All sample comparisons Do you really need to know every pairwise comparison within your population...we will dive into the nitty gritty details of comparison statistical tests – when to use them, what they...one-tailed test also works for exclusively less-than comparisons. Paired and unpaired t-tests Samples that come...family-wise error rate by only performing the comparisons you really care about. Family-wise error is the...probability of a false positive occurring when many comparisons are performed. For example, if your alpha is ...you have 5 samples and perform every pairwise comparison (10 in total), your likelihood of a false positive...higher than if you only look at 2 or 3 pairwise comparisons. Similar to family-wise error, is the false discovery...
  7. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...do an AMA in the first place and give you some reasons why you should consider using Reddit to share your... as our first foray into Reddit. Here are some reasons why: 1. Reddit has a large and active community...
  8. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ...research findings so difficult to do?  There are four reasons that research can be difficult to replicate. Unclear...with powerful cognitive biases such as motivated reasoning and hindsight bias, we may fool ourselves and ...Unanticipated discoveries Finally, there are good reasons for obtaining a different result after conducting...
  9. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...contact to connect on LinkedIn and always include a personal note about where you met and what you discussed...the near future. Even just one more meeting (in person or virtually) will make the relationship stronger... law of networking--don’t be creepy. There is a person on LinkedIn I once helped by answering one simple...not at all necessary. Once you’ve talked with a person a few times, if it was a positive experience for...was in graduate school, there was only one other person in my class who had children. When I had my baby...answered my questions and honestly, she was the only person who really understood what I was going through....
  10. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...conversations on the history of the group, lessons learned, and to Sonali Roy, Magdalena Julkowska, and Jennifer... provide greater visibility of these materials, Sonali Roy, a postdoctoral researcher at the Noble Research...effort. The future of reproducibility workshops and lessons learned: Since early 2018 the team has grown to...researchers at all levels of the scientific community. Lessons learned Instead of continuously talking about...
  11. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...Zhang group. Mechanism of CRISPR-transposons One of the CRISPR-transposon systems developed by the Sternberg...aka mini-transposon), which contains the DNA cargo of interest flanked by ~50-150 bp transposon end sequences...characteristic of the Tn7-family of transposons. Insertion of the mini-transposon can occur in two different orientations...with the same transposon exhibit reduced efficiencies (Vo et al., 2020). CRISPR-transposons within the CRISPR...Lab at Columbia University Medical Center. DNA transposons are ubiquitous genetic elements capable of spreading... Beauty). Despite their diverse utility, DNA transposons have been hindered by their limited programmability...recently surpassed with the discovery of CRISPR-transposons by the Sternberg and Zhang labs (Peters et al...
  12. The Advances Behind The World's First Personalized CRISPR Treatment

    Type
    Blog Post
    ...successfully treated with a CRISPR gene editing therapy personalized to his genetic mutation. “Baby KJ” was diagnosed...Ahrens-Nicklas administered the first dose of his personalized genetic medicine (Musunuru et al., 2025). Much... C., Rees, H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017). Programmable base ....2015.12.041  Musunuru, K., Grandinette, S. A., Wang, X., Hudson, T. R., Briseno, K., Berry, A. M., Hacker, J. L...Silverstein, R. A., Hille, L. T., Ogul, A. N., Robinson-Garvin, N. A., Small, J. C., McCague, S., Burke...https://doi.org/10.1056/NEJMoa2504747  Rees, H. A., Wilson, C., Doman, J. L., & Liu, D. R. (2019). Analysis..., Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer, D. E., Doudna...
  13. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    ...the next steps. Harvard PI Angela DePace has put personal development at the center of her lab (pictured...easily adaptable for use in your lab! Promoting personal development through thoughtful planning and honest... of in the supplement of this paper), help each person organize his or her thoughts to facilitate honest...through obstacles, and take steps to resolve interpersonal conflicts in the lab. Yearly planning meetings...hear about the steps taken in your lab to promote personal development - let us know your strategies in the... personal development...
  14. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ... said Addgene’s Lianna Swanson, who has been working with members of Davidson's lab to coordinate the ...work of Michael Davidson and his colleagues." Acknowledgments Thank you to Michael Davidson, Michelle Baird...and their use for imaging cell biology, Michael Davidson’s lab at Florida State University has been the ... easily searchable format on Addgene’s Michael Davidson Fluorescent Protein Collection webpage. These...who has created plasmids as a technician in the Davidson lab for the last four years. “Researchers can ...comprehensive list of readily available targets since the Davidson lab always tagged new proteins with mEmerald first...to localize to specific cellular structures.The Davidson lab even included some of their own reference ...
  15. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ...announce the recipients of this year’s Michael Davidson and Roger Tsien Commemorative Conference Awards...of their choice while honoring the late Michael Davidson and Roger Tsien who have played enormous roles...made some changes. In addition to supporting in-person conference attendance, the award now supports virtual... Aggarwal, and colleagues Filip Tomaska and Tim Hanson, are characterizing variants of iGluSnFR3 in vivo...
  16. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    ...the basics of Gibson assembly as a parody of “Breakfast at Tiffany’s.” Overview of Gibson assembly The ...The Gibson assembly technique was first described by Dr. Daniel Gibson and colleagues at the J. Craig Venter...program to design primers for Gibson Assembly. For a simple example of using Gibson assembly, imagine that you...the mixing and matching of parts. Gibson assembly meets CRISPR Gibson can be adapted to more complicated... a try! References: 1. Gibson DG, Young L, Chuang RY, Venter JC, Hutchison CA 3rd, Smith HO. Enzymatic... have become more and more common, and for good reason. They offer many advantages over the traditional...disadvantages, and examples of how scientists are using Gibson assembly to put together DNA fragments. As a fun...
  17. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    ...but also a biological transposon system. Sleeping Beauty is a synthetic transposon system that can be used...resurrecting this transposon system? Scientists can use Sleeping Beauty transposons as a non-viral system...Now, we have meetings in rooms such as Groucho, Sonic Hedgehog, Sleeping Beauty, Cookie Monster, Spaghetti...Shivdasani Lab from the Dana Farber Cancer Institute! Sonic Hedgehog, an important regulator of embryonic development...The largest conference room at Addgene is named Sonic Hedgehog but this is not a direct reference to the...gene in animal development. To understand where sonic hedgehog got its name, we need to go back to 1980...Wiechaus, 1980). The mammalian hedgehog protein, sonic hedgehog (SHH) was named by a postdoc in Cliff Tabin...
  18. AAV Production in HEK293 Cells

    Type
    Protocol
    ... of sonication to avoid overheating of the sample. Mix well between rounds of sonication. Sonicate until...container pH meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex Reagents Adherent HEK293T...completely. Combine all resuspended cell pellets and sonicate 5 x 1 sec pulses with at least 5 minutes on ice...
  19. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...Current Protocols in Protein Science. John Wiley & Sons, Inc. https://doi.org/10.1002/0471140864 Fox, J....
  20. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...family, he did not seem much different than my second son of similar age. When he came to visit us and watch...  Many thanks to our guest blogger Kurt Swanson! Kurt Swanson is a structural biologist and protein engineer...This post was contributed by Kurt Swanson a structural biologist and protein engineer currently working...
  21. Lentivirus ddPCR Titration

    Type
    Protocol
    ... two copies of RPP30 in diploid cells, thus the reason for multiplying by 2. $$V = 2*{copies\ RRE \over...Example dilutions and titrations Reference Wang Y, Bergelson S, Feschenko M. Determination of Lentiviral Infectious...
  22. Immunology Research Plasmids and Resources

    Type
    Collection
    ...RaLP, SHCD SOS1 son of sevenless homolog 1 (Drosophila) GF1, GGF1, GINGF, HGF, NS4 SOS2 son of sevenless ... RHOH12 SOS1 son of sevenless homolog 1 (Drosophila) GF1, GGF1, GINGF, HGF, NS4 SOS2 son of sevenless ...Set : From Jesse Boehm , William Hahn , Matthew Meyerson , and David Root , this kit consists of 182 wild-type..., Hu Z, Zalocusky KA, Shankar RD, Shen-Orr SS, Thomson E, Wiser J, Butte AJ. 2018. ImmPort, toward repurposing...
  23. Weighing Reagents Protocol

    Type
    Protocol
    ...of how to weigh reagents. Wear the appropriate personal protective equipment (PPE) prior to handling the...
  24. Video Library

    Type
    Protocol
    ...key strategies of aseptic technique, including personal protective equipment, setting up a clean workspace...
  25. Centrifugation

    Type
    Protocol
    ...Centrifuge Microfuge tubes or other suitable container Personal protective equipment (PPE) appropriate for the...
  26. Western Blot

    Type
    Protocol
    ...most proteins but more stringent buffers and a sonication step may be required for hard to extract proteins...
  27. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Myc CMV Parkinson's Mark Cookson 13314 pCMVTNT PINK1 C-myc PINK1 Myc CMV Parkinson's Mark Cookson 13315 ...CMV Parkinson's Mark Cookson 13316 pcDNA-DEST47 PINK1 C-GFP PINK1 GFP CMV Parkinson's Mark Cookson 13319...CMV Parkinson's Mark Cookson 13320 pLenti6-DEST PINK1-V5 WT PINK1 V5 CMV Parkinson's Mark Cookson 13321...GST tac Parkinson's Mark Cookson 13322 pGEX5X.1 PINK1 KD PINK1 GST tac Parkinson's Mark Cookson 13917 mSecPrP-pCB6...His, Myc CMV Parkinson's Ted Dawson 17612 pRK5-Myc-Parkin PRKN Myc CMV Parkinson's Ted Dawson 17613 pRK5...GFP CMV Parkinson's Mark Cookson 25045 pDEST53-LRRK2-G2019S LRRK2 GFP CMV Parkinson's Mark Cookson 25046...GFP CMV Parkinson's Mark Cookson 25048 pDEST53-LRRK2-Y1699C LRRK2 GFP CMV Parkinson's Mark Cookson 25049...
  28. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ... James M. Wilson AV-1-PV0105 105532-AAV1 pAAV.CMV.ffLuciferase.SV40 Control James M. Wilson AV-1-PV1917...James M. Wilson AV-1-PV1963 105542-AAV1 pENN.AAV.CB7.CI.eGFP.WPRE.rBG Control James M. Wilson AV-1-PV2177...James M. Wilson AV-1-PV2642 105552-AAV1 pENN.AAV.hSyn.TurboRFP.WPRE.RBG Control James M. Wilson AV-1-PV2975...Control James M. Wilson AV-2-PV0101 105530-AAV2 pAAV.CMV.PI.EGFP.WPRE.bGH Control James M. Wilson AV-2-PV1963...Control James M. Wilson AV-5-PV0102 105531-AAV5 pAAV.CMV.LacZ.bGH Control James M. Wilson AV-5-PV1917 105541...James M. Wilson AV-5-PV1963 105542-AAV5 pENN.AAV.CB7.CI.eGFP.WPRE.rBG Control James M. Wilson AV-5-PV2177... James M. Wilson AV-5-PV2213 105547-AAV5 pENN.AAV.EF1a.eGFP.WPRE.rBG Control James M. Wilson AV-5-PV2369...
  29. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...mCherry Maria Harrison 61206 pCMU-PDESr Plasmodesmata (Plants) PDLP1 mCherry Maria Harrison 61200 pCMU-PLAr...* Michael Davidson 54491 mCherry-Lifeact-7 Actin filaments LifeAct mCherry* Michael Davidson 54610 mEGFP-Lifeact...Michael Davidson 55148 mCherry-Tubulin-C-18 Microtubules alpha-Tubulin mCherry* Michael Davidson 12298 ...mCherry Maria Harrison *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent...Michael Davidson 55165 mCherry-ZO1-C-14 Tight junctions Zonula Occludens-1 mCherry* Michael Davidson 55001...TagRFP James Johnson 79801 pTag-BFP-C-h-Rab5a-c-Myc Endosomes (early) Rab5a TagBFP James Johnson 13050 DsRed-Rab5... 38770 pEF.myc.ER-E2-Crimson Endoplasmic reticulum ER retention signal E2-Crimson Benjamin Glick 36204...
  30. Brain Armamentarium

    Type
    Collection
    ...Alias: pAAV_WDC0004_eGFP Gordon Fishell James M. Wilson 221463-PHPeB pAAV_BiPVe4_eGFP AAV construct expressing...pAAV_WDL0003_dTomato_nlsdTomato Gordon Fishell James M. Wilson 218792-PHPeB pAAV_BiLAMP5e3_dTomato_nlsdTomato ...Alias: pAAV_WDS0004_GCaMP6f Gordon Fishell James M. Wilson 213947-PHPeB pAAV_BiSSTe4_GCaMP6f AAV construct...interneuron-targeting enhancer Gordon Fishell James M. Wilson 213945-PHPeB pAAV_BiSSTe4_ChR2_mCherry AAV construct...interneuron-targeting enhancer Gordon Fishell James M. Wilson 213944-PHPeB pAAV_BiSSTe4_dTomato_nlsdTomato AAV...Alias: pAAV_WDP0003_GCaMP6f Gordon Fishell James M. Wilson 213943-PHPeB pAAV_BiPVe3_GCaMP6f AAV construct ...cell-targeting enhancer Gordon Fishell James M. Wilson 213941-PHPeB pAAV_BiPVe3_ChR2_mCherry AAV construct...
  31. Optogenetics AAV Preps

    Type
    Collection
    ... Karl Deisseroth 122063 pAAV-EF1α1.1-ChrimsonR-GFP EF1a ChrimsonR GFP Constitutive 2 Edward Boyden 124650... 5, rg* Edward Boyden 59171 pAAV-Syn-ChrimsonR-tdT Syn ChrimsonR tdTomato Constitutive 1, 5, 9, rg* Edward...Edward Boyden 62723 pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] Syn ChrimsonR tdTomato Cre dependent 1, 5 Edward...Edward Boyden 130909 AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] CAG ChrimsonR tdTomato Flp dependent 8 Edward Boyden...dependent 9 Karl Deisseroth 92207 TRE-ChrimsonR-mCherry TRE ChrimsonR mCherry Constitutive 1 Alice Ting 105448... 105448 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Syn ChrimsonR (soma-targeted) mRuby2 Cre dependent 9 Hillel ...Adesnik 124603 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 EF1a ChrimsonR (soma-targeted) mRuby2 Cre ...
  32. CRISPR Guide

    Type
    Collection
    ... A., Chan, M. M., Bauer, D. E., Marson, A., Parsons, L. R., & Adamson, B. (2024). Improving prime editing...including transposons, integrases, and recombinases, with Cas enzymes. CRISPR Transposases Transposon systems...I., Yoon, Y., Song, C., Cao, Y., Gallant, J., Xue, W., Rivera-Pérez, J. A., & Sontheimer, E. J. (2019)...S., Cofsky, J. C., Kranzusch, P. J., Sontheimer, E. J., Davidson, A. R., Maxwell, K. L., & Doudna, J. ..., J., Edraki, A., Shah, M., Sontheimer, E. J., Maxwell, K. L., & Davidson, A. R. (2016). Naturally occurring... Chen, C., Nelson, J. W., Newby, G. A., Sahin, M., Osborn, M. J., Weissman, J. S., Adamson, B., & Liu,...Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., Pogson, A. N., Kim, J. Y., Chung, A., Leonetti...
  33. Control AAV Preps

    Type
    Collection
    ...PHP.eB James M. Wilson 105531 pAAV.CMV.LacZ.bGH CMV LacZ Constitutive 5, 8 James M. Wilson 105532 pAAV.CMV.ffLuciferase.SV40...Constitutive 8 James M. Wilson 105534 pAAV.TBG.PI.LacZ.bGH TBG LacZ Constitutive 8 James M. Wilson 105536 pAAV.TBG.PI.Null.bGH... 8 James M. Wilson 105539 pAAV.hSyn.eGFP.WPRE.bGH Syn EGFP Constitutive 1 James M. Wilson 200773 AAV-cfGFP...5 James M. Wilson 105535 pAAV.TBG.PI.eGFP.WPRE.bGH TBG EGFP Constitutive 8 James M. Wilson 105542 pENN.AAV.CB7...James M. Wilson 105543 pENN.AAV.cTNT.PI.eGFP.WPRE.rBG cTNT EGFP Constitutive 9 James M. Wilson 105544 pENN.AAV.CB7...James M. Wilson 105548 pENN.AAV.CMVs.TurboRFP.WPRE.RBG CMV TurboRFP Constitutive 1, 8 James M. Wilson 105549...James M. Wilson 105552 pENN.AAV.hSyn.TurboRFP.WPRE.RBG hSyn TurboRFP Constitutive 1 James M. Wilson 105554...
  34. Recombinases AAV Preps

    Type
    Collection
    ...James M. Wilson 105558 pENN.AAV.CamKII 0.4.Cre.SV40 CamKII none 1, 5, 9, rg* James M. Wilson 105537 pENN.AAV.CMVs.Pl.Cre.rBG...James M. Wilson 105545 pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 James M. Wilson 55632 pAAV-Ef1a-mCherry-IRES-Cre...James M. Wilson 105553 pENN.AAV.hSyn.Cre.WPRE.hGH Syn none 1, 2, 5, 8, 9, rg* James M. Wilson 105555 pENN.AAV.hSyn.Cre.hGH...pAAV.GFAP.Cre.WPRE.hGH GFAP none 5, PHPeB James M. Wilson 196410 AAV-GfaABC1D-Cre-4x6T GfaABC1D none 5 Stanley...107788 AAV.rTH.PI.Cre.SV40 rTH none 9, rg* James M. Wilson 24593 AAV-pgk-Cre PGK none rg* Patrick Aebischer... pENN.AAV.hSyn.Cre.hGH Syn none 9 James M. Wilson 107312 AAV-hSyn-mCherry-P2A-Cre-WPRE Syn mCherry 1 Hui... 107787 AAV.TBG.PI.Cre.rBGe TBG none 8 James M. Wilson Dre AAV ID Name Promoter Fluorophore Serotype(s...
  35. Bacterial Expression Systems

    Type
    Collection
    ...acceptor) FRET Robert Campbell , Michael Davidson Michael Davidson , Nathan Shaner , Roger Tsien 54575 54771...Timers Return to top Protein Interactions Förster resonance energy transfer (FRET) and bimolecular fluorescence...acceptor) FRET/Dual FRET Robert Campbell Michael Davidson , Nathan Shaner , Roger Tsien 54553 54723 mTFP1...acceptor) FRET/Dual FRET Robert Campbell , Michael Davidson 54571 54856 mT-Sapphire-pBAD tdTomato-pBAD mT-... Clover (donor) mRuby2 (acceptor) FRET Michael Davidson 18856 pGWF1 ECFP (donor) Venus (acceptor) FRET...IPTG Escherichia coli , Acinetobacter baumannii Jason Peters 127088 pMS17 tcp830 Anhydrotetracycline (... (firefly luciferase) Mycobacterium sp. Brian Robertson , Siouxsie Wiles 26161 pMV306hsp+LuxG13 Promoter...
  36. Brain Initiative Collection

    Type
    Collection
    ...105448-AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore...pAAV-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR under the ...pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE Expresses bicistronically soma-targeted ChrimsonR in frame...Chemogenetics Cre-dependent expression plasmid Scott Sternson 119741-AAV9 AAV SYN flex PSAM4 GlyR IRES EGFP ...Chemogenetics Cre-dependent expression plasmid Scott Sternson 119742-AAV5 AAV SYN PSAM4 GlyR IRES EGFP Chemogenetics...Chemogenetics expression plasmid Scott Sternson 119744-AAV5 AAV CAMKII PSAM4 GlyR IRES EGFP Chemogenetics...
  37. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR. 2016. Naturally occurring off-switches...MF, Hidalgo-Reyes Y, Wiedenheft B, Maxwell KL, Davidson AR. 2015. Multiple mechanisms for CRISPR-Cas inhibition...26416740 Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR. 2013. Bacteriophage genes that inactivate ...132-6. PMID: 23942116 Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead EH, Guimaraes...Cell . 159(3):647-6. PMID: 25307932 Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-...Topkar VV, Nguyen NT, Zheng Z, Gonzales AP, Li Z, Peterson RT, Yeh JR, Aryee MJ, Joung JK. 2015. Engineered...Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. 2015. Multicolor CRISPR labeling of chromosomal...
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