We narrowed to 54 results for: aav gfp cre

...efficiency of AAV-PHP.B versus AAV-PHP.eB in the CNS. A GFP transgene was packaged into either an AAV-PHP.B or... comparison, capsids were packed with a GFP transgene and GFP intensity and transduction efficiency of...efficiency of AAV-PHP.eB and AAV-PHP.S versus their parental AAV vectors. What can these AAV’s be used for...efficiency of AAV.9 versus AAV.PHP.S in the PNS. A GFP transgene was packaged into either an AAV.9 or an AAV.PHP.S...stands for Cre Recombinase-based AAV Targeted Evolution. In this technique, a pool of mutant AAV capsid (...Search for AAV Vectors to use with your research. Visit Our AAV Guide Page for info on AAV components,... with cell-type-specific Cre, the polyA sequence is inverted only in the Cre-expressing tissues. This ...

...GBPs/GFP. The new system, CRE-DOG (Cre Dependent On GFP), is activated by GFP and derivatives GFP and ...to bind GFP. The idea of using GFP as a scaffold suddenly seemed very realistic. Envisioning GFP as a substrate...Connie Cepko Combining Cre and GFP with Cre-DOGs The T-DDOG results showed that GFP could regulate transcription...induced in ~76% of GFP+ cells, and 100% of DsRed+ cells also expressed GFP. Using AAV constructs, Tang ...At Addgene, we love GFP, and we’re always excited when depositors find new ways to make this workhorse...transcription or Cre recombinase activity only in the presence of GFP. These systems, known as T-DDOG and Cre-DOG,...lines expressing GFP in specific cell types to do more than just label cells. If GFP could be co-opted...

... how Cre-lox recombination works. Advances in Cre-lox recombination have included inducible Cre, such...transgenes and Cre, Cai et al. also created an Autobow construct containing both Cre and XFPs. Cre production...adeno-associated viral vectors (AAV) available - AAV-EF1a-BbChT and AAV-EF1a-BbTagBY. These constructs ...continued utility of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need a quick...membrane tethered cyan). Without Cre recombination, RFP is expressed. Cre can mediate one of two deletions...construct, OFP (orange), is expressed when Cre is absent; stochastic Cre excision results in one of three recombinations...system employs Cre-mediated deletion, the design of Brainbow-2.1 cleverly combines Cre-mediated deletion...

...channelrhodopsin, fused to a fluorescent GFP marker (CatCH-GFP). The lab deposited AAV plasmids containing the active... of Cre-mCherry and a second promoter to drive expression of a Cre-dependent fluorescent CatCH-GFP reporter...Expression of GFP will only result in cells where Cre-mCherry is also expressed. If Cre-mCherry expresses...horizontal cells transduced by both AAVs would express Cre-mCherry and GFP.   Figure 2: Synthetic promoters... photoreceptors and horizontal cells, and Cre-dependent-GFP expresses in horizontal cells and ganglion...Adeno-associated virus (AAV) is a popular tool for gene delivery, but it has a drawback: how do you ensure...Performance of synthetic promoter AAVs in mice All of these synthetic promoter AAVs were first injected into the...

...makes an AAV transfer plasmid an AAV transfer plasmid. ITR sequences are 145 bases each and AAV plasmids... want to do, but it requires some AAV. You’ve never worked with AAV before, but you aren’t going to let...experiments, making AAV starts with some plasmids. You just need three plasmids to start making AAV:  the packaging... the AAV transfer plasmid and take a look at each of its parts. Figure 1: Parts of an AAV transfer...   Key elements of an AAV plasmid First let’s talk about the key parts of an AAV plasmid. Inverted terminal...packaged into the AAV molecule. What’s outside of the ITRs does not get packaged in the AAV vector. The cloning...ITR loss abolishing AAV packaging. To prevent ITR loss, it’s best to propagate AAV constructs in recombination-deficient...

...consider our AAV Packaged on Request service for more options. Addgene blog Plasmids 101: Cre-lox Cre-ating ...Controlling Protein Activity with GFP Plasmids 101: FLEx Vectors Optimizing AAV DIO and FLEx Vector Expression...Plasmid Collections Recombinases Cre-Lox and Other Site-Specific Recombinases Cre Plasmids Flp Plasmids Dre ...prevent gene expression in the absence of Cre. In the presence of Cre, the stop codon is excised, and gene ...until Cre is present, at which time the gene will be disrupted or deleted. shRNA Expression: Cre-lox can...interest, Genes A and B. When Cre is absent, only Gene A is translated correctly. Cre expression excises Gene...double-crossover event. Toxicity Cre-lox recombination is a very useful tool, but Cre expression can be toxic ...

...EF1a Nuc-flox(mCherry)-EGFP Brandon Harvey 50457 AAV2 pAAV-hSyn-DIO-EGFP Bryan Roth 50459 AAV1 pAAV-hSyn-DIO-mCherry..., AAV Retrograde, AAV2, AAV5, AAV8, and AAV9). Once activated, this DREADD acts through the Gq pathway...respectively of a GFP dependent Cre. The two segments come together in the presence of GFP to form a functional...gene activation to cells expressing both Cre and GFP. The GFP dependent FLPo, Flp-DOG (75469), is also...proteins ChR2 for targeted expression DREADDs GFP dependent Cre and FLPo And more!  New lentiviral preps...plasmids in new serotypes, as well as a new Cre dependent GFP control plasmid for expression in forebrain...Serotype Name 114471 AAV2 pAAV-Ef1a-fDIO mCherry 83900 AAVrg pAAV-mDlx-GFP-Fishell-1 Biosensors...

... 20 ul aliquots in AAV1, AAV2, AAV5, AAV8, AAV9, and AAV Retrograde. You can also request the specific...: pGP-AAV-syn-jGCaMP7f-WPRE (104488-AAV8) pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (104492-AAV8) pGP-AAV-syn-jGCaMP7c...Flp-dependent Cre AAV in AAV9 and AAVrg. See our new additions here: Cre vectors pAAV-EF1a-fDIO-Cre (121675...26976-AAV5) pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5) Calcium Sensors pGP-AAV-syn-jGCaMP7b-WPRE.... Our new AAVs include: EGFP-expressing AAV for serotype testing Calcium sensors and GABA sensors Optogenetics...is now complete! pAAV-CAG-GFP (plasmid 37825) and pAAV-hSyn-EGFP (plasmid 50465) are now available as 20...pAAV-hSyn-DIO-EGFP (50457-AAV5): The Synapsin promoter directs broad, neuronal expression. AAV pCAG-FLEX-...

...prep pAAV-CAG-GFP AAV9-X1.1 Controls Edward Boyden New serotype pAAV-hSyn-EGFP AAV PHP.eB Controls...levels compared to AAV9. You can now target these cells with AAV-CAG-GFP packaged in AAV9-X1.1!  We also ...pAAV-EF1a-fDIO-Cre AAV5 Recombinases Esteban Engel New serotype pAAV-hSyn-Cre-P2A-dTomato AAV PHP.eB Recombinases... Serotype Category Depositor  Notes AAV-GfaABC1D-Cre-4x6T AAV5 Recombinases Stanley Thomas Carmichael... (Alias: CN3905) AAV PHP.eB Controls Jonathan Ting New viral prep pAAV-CaMKIIa-EGFP AAV1 Controls Bryan...H134R)-EYFP AAV2 Optogenetics Karl Deisseroth New serotype AAV-CamKIIa-jGCaMP8s-WPRE AAV5 Biosensors ...first Addgene prep packaged in the AAV9-X1.1 serotype.  In rodents, AAV9-X1.1 enables expression in brain...

...diversified) 37825  AAV8*, AAV9*  pAAV-CAG-GFP 50465  AAV8*, AAV9*  pAAV-hSyn-EGFP *Also coming in...pAAV-mDlx-NLS-mRuby2 CAG-NLS-GFP pAAV-hSyn-mCherry Read on to learn more!  Calcium sensor AAV The GFP-based calcium...pAAV-Syn-Archon1-KGC-GFP-ER2) and cre-dependent version (pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]) of this membrane-embedding... 50363  AAV5  AAV phSyn1(S)-DreO-bGHpA 121675  AAV9  pAAV-EF1a-fDIO-Cre (FLP-dependent CRE) 55634...pAAV.hSyn-FLEX.iGABASnFR.F102G pAAV-Syn-Archon1-KGC-GFP-ER2 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] pAAV-FLEX-tdTomato pAAV-GFAP104...SYN1 HA-hM4D(Gi) 119741  AAV5  AAV SYN flex PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation...manipulation 119742  AAV5  AAV SYN PSAM4 GlyR IRES EGFP Chimeric channels for neuronal manipulation Recombinases...

...Schnutgen et al. Nat Biotech 2003): In the presence of Cre, GFP expression is lost, allowing for rescued expression...well as for improvement of optogenetic adeno-associated virus (AAV) vectors. Listed below are a few additional... should contain a reporter DNA sequence (such as GFP) in the sense orientation, followed by coding sequence...express GeneR only in the presence of Cre. In this example, Cre would first invert the reporter and GeneR...a cell or tissue-specific Cre is provided. Since the FLEx switch ensures Cre-dependent reporter expression...orientation in the presence of Cre. A similar strategy can be employed for "Cre-off" (i.e. your gene of interest...in the sense orientation and Cre is used to flip it to "off"). These Cre-off switches may be referred ...

...Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the absence of Cre. mCherry...[Jaws-KGC-GFP-ER2] (Addgene 84445-AAVrg) alone at 1.1E6 vg/mL, or both. Two days later, Cre-dependent ... and quality control are described below. AAV Production AAV distributed by Addgene has been produced ...purity of the AAV preparation. Figure 1: Silver staining of purified and non-purified AAV subjected...   Figure 2: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone...prep # 55632-AAVrg). pAAV-CAG- FLEX-rc [Jaws-KGC-GFP-ER2] was a gift from Edward Boyden (Addgene viral...arrowhead). Scale bar = 100 nm.   AAV resources Browse AAV preps available from Addgene. After receiving...

...transduced at levels similar to that of a single AAV expressing GFP. The lab tested the base editors in vivo ...combines the Cre/lox system with optogenetics. Cre activity is controlled by red light by splitting Cre and fusing...here.        AAV delivery of split base editors Article contributed by Jennifer Tsang AAVs are a great ...voltage indicators, however, fall short compared to GFP-based sensors in terms of response and brightness...reduces toxicity as compared to previous Cre/lox or drug-induced Cre systems (ex: tamoxifen). As the name ... dimerize, bringing each terminus of Cre together to restore Cre activity. As soon as red light or PCB...proteins separate, inactivating Cre. This is critical for reducing Cre toxicity in long-term experiments...

...) pAAV-CAG-GFP 50465 AAV8, 9 (20 µL size serotype testing) AAV-hSyn-EGFP Biosensor AAV The ability...popular control AAV encoding GFP. Check out our inventory of serotype testing AAV New control AAVs Plasmid...AAV1 pAAV-CAG-GFP 59462  AAV2 pAAV-CAG-tdTomato (codon diversified) 114472  AAV5, AAV8 pAAV-hSyn-mCherry...Serotype Name 119741  AAV5  AAV SYN flex PSAM4 GlyR IRES EGFP Recombinase AAV Finally, we are excited to...Flp-expressing AAV, as well as a Cre-dependent Flp-expressing AAV. See our recombinase AAV inventory here...testing) pAAV-CAG-GFP 50465 AAV1, 2, 5, rg (20 µL size serotype testing) pAAV-hSyn-EGFP Recombinases...Name 99039  AAVrg pAAV-CamKII-ArchT-GFP (PV2527) 105679  AAV5 ppAAV-CKIIa-stGtACR1-FusionRed New...

...line expressing a GFP-tagged version of a protein of interest. The binding of the GFP nanobody to its target... proximity to the GFP-tagged protein (albeit slightly offset due to the nanobody-GFP interaction). The...labeling Increased neuronal expression using Cre-mediated AAV Targeted Evolution method (M-CREATE) dCas9...ligase TurboID fused to a conditionally stabilized GFP-nanobody rather than a specific protein of interest...-4.   Increased neuronal expression using Cre-mediated AAV Targeted Evolution method (M-CREATE) by: Brian...previously developed the multiplexed version of the Cre-mediated AAV Targeted Evolution method (M-CREATE), in which...generational evolution of the AAV capsids (left) and transduction patterns (right).  Both AAV-PHP.eB and the AAV....

... Mammalian shRNA Tools for RNAi! Cre-Lox technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral ...retroviral vector from the Gage lab encodes GFP and Cre-recombinase. The Cre-lox system is a technology that can...gamma-retrovirus, lentivirus, Adenovirus, and Adeno-associated virus (AAV). If you are wondering which virus fits...Vectors   Expressing your gene of interest pMKO.1 GFP (Plasmid #10676) Retroviral vector for cloning and...and expressing your gene of interest with GFP expression.  Cloning and expressing RNAi pMKO.1 puro (Plasmid...of mouse transgenics. Experimental uses include: Cre-dependent gene expression, gene knockout, and selection... how to use the pLKO.1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408...

...fused to GFP forms fluorescent biomolecular condensates. A GFP-nanobody fused to the killswitch (GFP-nb–KS...the EGFP, and the TEV protease. Once the recipient cells take up the Cre-tagged mitochondria, Cre is released...protein of interest or targeted to a GFP fusion protein using an anti-GFP nanobody (Figure 4). Once incorporated... excises the DsRed-STOP to trigger expression of EGFP. Figure 2: Schematic of the MitoTRACER system...Micropeptide killswitch for biomolecular condensates AAV-CRISPRa-engineered fat cells fight cancer Frugal ...interactions. Plus, the tools used here (also available as AAV Packaged on Request) will be useful for related studies...mitochondria (Hoover et al., 2025). MitoTRACER relies on a Cre-lox switch to turn recipient cells from red to green...

...PI 37825 AAV-CAG-GFP CAG GFP Control Edward Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP, Cre-dependent... 50465 pAAV-hSyn-EGFP Syn EGFP Control Bryan Roth 50469 pAAV-CaMKIIa-EGFP CamKII EGFP Control Bryan Roth...pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Gordon Fishell 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Control...pAAV-hSyn-DIO-EGFP Syn EGFP, Cre-dependent Control Bryan Roth 55650 pAAV-hSyn Con/Fon EYFP Syn EYFP, Cre and Flp-dependent...pENN.AAV.EF1a.eGFP.WPRE.rBG EF1a EGFP Control James M. Wilson 116869 pAAV-CAG-H2B-GFP CAG GFP Control Loren Looger...24593 AAV-pgk-Cre PGK Cre expression Recombinases Patrick Aebischer 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a...pAAV-hsyn-Jaws-KGC-GFP-ER2 Syn Inhibitor Optogenetics Edward Boyden 84445 pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] CAG...

...CAG-NLS-GFP CAG NLS-GFP Constitutive PHPeB Viviana Gradinaru 116869 pAAV-CAG-H2B-GFP CAG H2B-GFP Constitutive...pAAV-EF1a-mitoGFP-DIO EF1a mito-GFP Cre dependent 2 Maarten Kole 190155 AAV_pMBP-EGFP-caax MBP EGFP-caax Constitutive...mCherry Cre dependent 1, 2, 5, 8, 9, rg* Bryan Roth 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP Cre dependent...pAAV-hSyn-EGFP hSyn EGFP Constitutive 1, 2, 5, 8, 9, 11, rg*, PHP.eB Bryan Roth 50469 pAAV-CaMKIIa-EGFP CaMKIIa...EF1a mScarlet Cre dependent 1, 5 Karl Deisseroth 50457 pAAV-hSyn-DIO-EGFP hSyn EGFP Cre dependent 1, 2...-CAG-FLEX-EGFP CAG EGFP Cre dependent 1 Ian Wickersham 104052 pAAV-CAG-DIO-EYFP CAG EYFP Cre dependent...Fluorophore/Tag Activity Serotype PI 37825 AAV-CAG-GFP CAG GFP Constitutive 1, 2, 5, 6, 8, 9, 11, rg*, PHPeB...

...optogenetic AAV for expression in forebrain GABA-ergic interneurons. These include mDlx-GFP, hDlx-FLEX-GFP, hDlx-Flex-dTomato...syn-FLEX-axon-jYCaMP1s. Find these AAVs at Addgene Cre-dependent EYFP AAV in the new serotype PHP.V1 that...introduced into the hPSC genome using AAV1 and contain an EGFP reporter. Expression of the dCas9-KRAB activator...vesicular brain cells. Find this AAV at Addgene New serotypes for the Cre-dependent Flpo recombinase pEF1a-DIO-FLPo-WPRE-hGHpA...pEF1a-DIO-FLPo-WPRE-hGHpA, which is now also available in AAV5 and AAVrg. Find these AAVs at Addgene New controls, biosensor...plasmids at Addgene! CRISPR RNP electroporation and AAV donor infection (CRISPR-READI) enables genome engineering...sensors include syn-jYCaMP1s (slow variant), the Cre-dependent syn-FLEX-jYCaMP1s, and the axon-targeted...

...Chronos-eGFP]-WPRE EF1a Chronos EGFP Flp dependent 2 Ivo Spiegel 22222 AAV-FLEX-Arch-GFP CAG Arch GFP Cre ...pAAV-hsyn-Jaws-KGC-GFP-ER2 Syn Jaws GFP Constitutive 5, 8, rg* Edward Boyden 84445 pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2...Activity Cre dependent Flp dependent Cre and Flp dependent Constitutive Serotype AAV1 AAV2 AAV5 AAV8 AAV9 ...PdCO mScarlet Cre dependent 1, 5 Ofer Yizhar 198513 pAAV_hSyn-PdCO-EGFP-WPRE Syn PdCO EGFP Constitutive...soma-targeted) GFP Constitutive 9 Edward Boyden 107712 pAAV-hSynapsin-FLEX-soCoChR-GFP Syn CoChR (soma-...Deisseroth 122063 pAAV-EF1α1.1-ChrimsonR-GFP EF1a ChrimsonR GFP Constitutive 2 Edward Boyden 124650 pAAV-CamKIIa-C1V1...Christopher Harvey 59170 pAAV-Syn-Chronos-GFP Syn Chronos GFP Constitutive 1, 5, rg* Edward Boyden 59171...

... a 'Cre-on' dependent manner. 1 Don Arnold 124364 pAAV-FLEX-DTR-GFP CBA-driven, Cre-dependent Cre-dependent...2A-Synaptophysin-mRuby Syn-driven, Cre-dependent Cre-dependent expression of membrane-bound GFP and synaptophysin-mRuby... 98747 pAAV-FLEX-EGFPL10a EF1a-driven and Cre-dependent EGFP-tagged ribosomal L10a. 5, rg* Nathaniel Heintz...Serotype PI 51509 AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE Syn-driven, Cre-dependent Cre-dependent expression...with cysteine-free GFP (cfGFP) under synapsin promoter. 8 Alexander Dityatev 200778 AAV-∆Hapln1 hSyn-driven...99121 pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible Synapsin promoter (ihSyn) Cre-dependent expression of...Gradinaru 117383 TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible Cre-dependent, Tet-inducible expression...

... Gether 119741 AAV SYN flex PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP Cre-dependent 5, 9 ...mCitrine EGFP dTomato Activity Cre-dependent Flp-dependent Cre and Flp-dependent Constitutive (non-cre dependent...dependent) Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity...Scott Sternson 119742 AAV SYN PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Scott Sternson...Sternson 119744 AAV CAMKII PSAM4 GlyR IRES EGFP PSAM4 GlyR - Inhibition IRES EGFP none 5 Scott Sternson 121538...52536 rAAV-CAG::FLEX-rev:: hM4D-2a-GFP hM4D(Gi) - Inhibition GFP Cre-dependent 1 Scott Sternson 228902 ...Service AAV Chemogenetics Viral Vector Packaging Service: Chemogenetics AAV Use our chemogenetics AAV to chemically...

...pAAV-CAG-FLEX-Archon1-KGC-EGFP-ER2 CAG Archon1 EGFP Cre dependent 5 Edward Boyden 115892 pAAV-Syn-Archon1-KGC-GFP-ER2 Syn... Constitutive (non-cre dependent) Serotype AAV1 AAV2 AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters...HaloCaMP1a 138327 pAAV-synapsin-HaloCaMP1a-EGFP Syn HaloCaMP1a EGFP Constitutive 1, 9 Eric Schreiter Calcium...HaloCaMP1b 138328 pAAV-synapsin-HaloCaMP1b-EGFP Syn HaloCaMP1b EGFP Constitutive 1, 9 Eric Schreiter Dopamine...Syn Archon1 EGFP Constitutive 8 Edward Boyden 115893 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] Syn Archon1...Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor AAV Browse our AAV that express ...Synapsin Other Activity Cre-dependent Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent...

...Service AAV Recombinases Viral Vector Packaging Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible... M. Wilson 24593 AAV-pgk-Cre PGK none rg* Patrick Aebischer 51507 AAV pmSyn1-EBFP-Cre Syn EBFP 5 Hongkui...expression. See our Cre-lox Plasmids page for more information about these molecular tools. Cre AAV ID Name Promoter...pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 CMV eGFP 1, 2, 5, 8, 9 James M. Wilson 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a mCherry (not...Wilson 107312 AAV-hSyn-mCherry-P2A-Cre-WPRE Syn mCherry 1 Hui Yang 107738 pAAV-hSyn-Cre-P2A-dTomato Syn...provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of site...Light-Inducible Recombinases VCre AAV Browse the following tables for in-stock AAV preps that encode site-specific...

...generation of iGluSnFR, the Looger lab swapped eGFP for sfGFP to create SF-iGluSnFR. This improved variant...regulation. To create iGluSnFR, the Looger lab wedged GFP between two halves of a bacterially derived periplasmic...increase in fluorescence intensity from the inserted GFP. After characterizing iGluSnFR in vitro, showing ...Formulated as AAV-DJ for neuronal expression. CAG-FLEX: CAG promoter, FLEXed for expression in Cre-expressing...and beyond. These new iGluSnFR3 variants come in AAV packaged virus aliquots being deposited at Addgene...hSynap-FLEX: Synapsin-1 promoter, FLEXed for expression in Cre-expressing cells. hSynap-FLP: Synapsin-1 promoter... Biosensors Browse All Fluorescent Proteins Find AAV Tools References Marvin, Jonathan S., et al. “An...

...mCherry)-EGFP 27056 AAVrg pAAV-Ef1a-DIO EYFP 50457 AAV2 pAAV-hSyn-DIO-EGFP   Biosensor AAV Calcium...50457 AAV2 pAAV-hSyn-DIO-EGFP Recombinases Plasmid Serotype Name 87306 AAVrg, AAV5 AAV pEF1a-DIO-.... Our new AAVs include: Optogenetics AAV Control AAV Biosensor AAV And more! Optogenetics Optogenetics...Testing AAV inventory for the entire collection! Plasmid Serotype Name 51502 AAV5 pCAG-FLEX-EGFP-WPRE... 114471 AAV5 pAAV-Ef1a-fDIO mCherry 112677 AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP 50457...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE 104496 AAVrg pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE 104495 AAVrg GP-AAV-CAG-FLEX-jGCaMP7s-WPRE...Name 113050 AAV9, AAVrg pAAV-hSyn-GRAB_DA1h 113049 AAV9 pAAV-hSyn-GRAB_DA1m 123308 AAV9, AAVrg pAAV-hSyn-GRAB_NE1m...

...S29W.A95K.HaloTag AAV5 Biosensor Tim Brown New viral prep pGP-AAV-syn-flex-iGABASnFR2(no bind)-WPRE AAV1, AAV5 Biosensor... AAV1, AAV2, AAV9 Biosensor Robert Campbell New viral prep with multiple serotypes AAV-hSyn-Ribo-jGCaMP8s...: pAAV-AiE0779m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4033) AAV-PHPeB Optogenetics Jonathan Ting...: pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035) AAV-PHPeB Optogenetics Jonathan Ting...pAAV-AiE0873m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5140) AAV-PHPeB Optogenetics Jonathan Ting New viral prep...prep AAV-hSyn-Ribo-jGCaMP8m AAV1 Biosensor Marianne Fyhn New viral prep pAAV-Ef1a-fDIO EYFP AAV5. AAV8...serotype (multiple) rAAV-CAG::FLEX-rev:: hM4D-2a-GFP AAV1 Chemogenetics Scott Sternson New viral prep ...

... from the default GFP (green) to RFP, CFP, or YFP fluorescence. In Brainbow 2.1, GFP is tagged with a ...Tissue-specific Cre recombination in this mouse generates cells that randomly express either nuclear GFP, membrane...incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red) to solve the problematically low...and activate fluorescence of the downstream OFP, GFP, or RFP. Cells that express the construct but do ...protein genes, Cre recombination is required before fluorescence can occur. Cre recombinase can be used ...developing fish via Cre protein microinjection or crossing of Zebrabow animals to an inducible Cre line. The Schier...OFP (orange) (Livet et al., 2007). Brainbow uses a Cre/lox system to label neurons. Genes encoding the fluorescent...

...Voltron2 voltage sensors AAV vectors for targeting microglia The REVeRT Dual AAV Vector System Neurodegeneration...investigators found that administering these AAVs with a dilute Cre-expressing vector resulted in a sparsely-labeled... incorporated in an AAV vector (Figure 5). Efficiency and specificity of these AAV vectors for microglia...self-complementary AAV (scAAV). However, scAAV vectors have limited size compared to single-strand AAV (ssAAV). ...Figure 5: A) Representative confocal images showing GFP expression from ssAAV5s with hIBA1 or hIBA1a promoters...Quantifications showing high microglia specificity of GFP expression for the indicated ssAAV5s. C) Quantifications...Dual AAV Vector System By Vaibhav Kawde To circumvent the payload limitations of traditional AAVs, the...

...dim GFPs The availability of the three dimensional structure of GFP, its brighter mutant S65T-GFP, and...carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre alleles showed efficient GFP knockdown in eyes and clonogenic...sequence in between two fragments of EGFP in pCAG-EGxxFP. The EGFP fragments contain 482bp of overlapping...strain in which Cre expression causes the induction of both DsRed fluorescence and GFP shRNA. Mice carrying...and demonstrate that they can successfully create GFP fusion proteins with a variety of genes across the...of Dimitri Deheyn recently identified a family of GFP proteins in the cephalochordate Branchiostoma floridae...Cas9 gene editing. Dr. Masahito Ikawa has created a GFP reporter plasmid for scientists to validate the efficacy...

...Cell type-specific manipulation with GFP-dependent Cre recombinase.” Nat. Neurosci. 18(9) (2015): 1334-...about Professor Cepko's recent work on developing GFP-activated proteins, read our recent blog post. Check...Multiple Genes to Cells for Gene Therapy 28:40-35:10 : GFP Activatable Tools Being Developed in the Cepko Lab...on the Addgene Blog Read our Recent Blog Post on GFP Activable Plasmid Tools Being Developed in the Cepko... with Michael Koeris Get Cepko Lab Tips on using AAVs in Your Experiments  Resources on the Addgene Website...

...license. Libraries are available with T-Sapphire, EGFP, or mScarlet reporters, so you can choose a color...our blog post on AAV serotypes for more about the tissue tropism of these and other AAVs. Figure...them in our own AAV production processes. Check out our step-by-step protocols for AAV production, purification... In-Stock AAV inventory or use our Packaged on Request service for thousands of eligible AAV plasmids ...packaging plasmids from Addgene By Meghan Rego Attention AAV users: in an effort to expand your gene delivery ...postsynaptic AMPA receptor and is endocytosed, the Cre recombinase payload activates a floxed reporter gene (genetically...created with BioRender.com. When delivered by AAV to various brain regions in mice, ATLAS mediated ...

...ones? Many of the available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free...Applications Viral Vectors 101: An Introduction to AAV Find AAV Calcium Biosensor Vectors at Addgene ...Calcium is arguably one of the most important metal ions in biology (sodium and potassium are up there... expression, and compatible with constitutive or Cre-dependent expression. These sensors have also been... tissue-specific targeting. By employing various AAV serotypes, researchers can precisely direct the calcium... ready to go. Pro tip: Vectors with ready-to-use AAV preparations are labeled with the green virus particle...3):1057-65. Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’...

...available in AAV8! Expand your imaging experiments with the Cre-OFF/FLP-ON vector 137128-AAV8: Ef1a-Coff...SL-sensitive fluorophore (circularly permuted green fluorescent protein or cpGFP) to the petunia SL enzymatic ...selection of GCaMP8 AAV preps has expanded. Check out the new selection of ready-to-use AAV9 and AAVrg vectors...

...restraint include an AAV-based sparse labeling system where a limiting amount of AAV are injected into the...transcription activation systems, such as LexA/p65 or mCD8/GFP, to allow sparse labeling in a variety of fly cell... the brain of Cre-expressing mice to trigger recombination and expression of a transgene (Lin et al., ...However, this system requires precise titration of the AAV. So far, tools to overcome this challenge in Drosophila...

...along with EGFP while retaining an emission spectrum that is easily separable from that of EGFP. This makes...spectra (N- and C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that...-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-linked...and combined it with a variety of reporters (i.e. eGFP) and effectors (i.e. DREADDs). The team demonstrated...mCyFP1, the Lin and Yasuda groups use both mCyFP1 and EGFP as FRET donors in experiments simultaneously measuring...interneurons, Gordon Fishell’s lab has developed a new AAV approach using regulatory elements that restrict ...to this cell type. To this end, they constructed AAV plasmids bearing one of the distal-less homeobox ...

...tdTomato, Cre-dependent Control Boyden 37825 pAAV-CAG-GFP CAG GFP Control Boyden 50465 pAAV-hSyn-EGFP hSyn ...hSyn EGFP Control Roth 50469 pAAV-CaMKIIa-EGFP CamKIIa EGFP Control Roth 59462 pAAV-CAG-tdTomato CAG tdTomato...104061 CAG-NLS-GFP CAG NLS-GFP Control Gradinaru 105530 pAAV.CMV.PI.EGFP.WPRE.bGH CMV EGFP Control Wilson...pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn Cre-EGFP expression Cre Wilson 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP Cre-expression Cre Wilson 107738...-B22 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden AAV9-X1.1 ...Available MaCPNS1 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden ...Available MaCPNS2 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden ...

...Gordon Fishell 83895-AAV2 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain...Gordon Fishell 83895-AAV8 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain...Gordon Fishell 83895-AAV9 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain...CAG-NLS-GFP AAV expression of nuclear localized GFP with SV40 NLS Viviana Gradinaru 105448-AAV9 pAAV-hSyn-DIO-ChrimsonR-mRuby2...Yulong Li 115892-AAV8 pAAV-Syn-Archon1-KGC-GFP-ER2 AAV-mediated expression of Archon1-KGC-GFP-ER2 under the... 115893-AAV8 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] AAV-mediated expression of Archon1-KGC-GFP-ER2 under...Fishell 83895-AAV1 pAAV-hDlx-Flex-GFP-Fishell_6 Cre recombinase-dependent GFP expression in forebrain GABA-...

...target gene expression (EGFP, in the depositing manuscript) across the brain. AAV-BI-28 has been tested ...astrocytes (60%) than AAV9 (Ravindra Kumar et al., 2020). AAV9-X1.1 is evolved from AAV9 and transduces GLUT1...    Figure 1: AAV toolkit for targeted gene delivery to the brain. The AAV (a) capsid, (b) cargo...However, these natural serotypes (AAV9, AAVrh10, and to a certain extent, AAV8) have low transduction efficiency...capsids are derived from AAV9, their efficacy is generally compared to the AAV9 serotype or an engineered...Systemic capsids packaging Cre-dependent transgenes can also be paired with Cre-expressing transgenic mouse...precious cargo space in AAV vectors. Dual expression systems, which use two or more AAVs to transduce the same...

...James M. Wilson 37825-CAP-B10 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana...Viviana Gradinaru 37825-CAP-B22 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana... Gradinaru 37825-CAP-MaCPNS1 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana... Gradinaru 37825-CAP-MaCPNS2 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana...pAAV-AiE0873m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5140) Expression of CoChR-EGFP in striatal cholinergic...pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035) Expression of CoChR-EGFP in striatal indirect pathway...pAAV-AiE0779m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4033) Expression of CoChR-EGFP in striatal direct pathway...

...(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This is a AAV plasmid for sgRNA cloning. GFP-KASH fusion ...activator with 2A GFP 61423 : Expresses the MS2-P65-HSF1 activator helper complex with 2A GFP 61424 : sgRNA...backbone with Cre recombinase 60230 : sgRNA targeted to NeuN, plus Cre recombinase-2A-EGFP-KASH 60231 :...ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR This plasmid enables Cre/loxP recombination and...cassettes, Cre recombinase-2A-EGFP-KASH and an sgRNA. #60231 - AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR... : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone cloning information: CRISPR-Cas9...plasmid contains two expression cassettes, Cre recombinase-2A-EGFP-KASH and an sgRNA backbone for cloning ...

...22222-AAV9 AAV-FLEX-Arch-GFP Optogenetics Ed Boyden AV-9-PV2527 99039-AAV9 pAAV-CamKII-ArchT-GFP (PV2527...PV2509 29777-AAV9 pAAV-CAG-ArchT-GFP Ed Boyden AV-9-PV3446 59170-AAV9 pAAV-Syn-Chronos-GFP Ed Boyden AV...27056-AAV2 pAAV-Ef1a-DIO EYFP Control Karl Deisseroth AV-2-ALL854 51502-AAV2 AAV pCAG-FLEX-EGFP-WPRE Control...-ALL854 51502-AAV9 AAV pCAG-FLEX-EGFP-WPRE Control Hongkui Zeng AV-9-PV0101 105530-AAV9 pAAV.CMV.PI.EGFP.WPRE.bGH...22222-AAV1 AAV-FLEX-Arch-GFP Optogenetics Ed Boyden AV-1-PV2527 99039-AAV1 pAAV-CamKII-ArchT-GFP (PV2527)...Sternson AV-5-PV2432 22222-AAV5 AAV-FLEX-Arch-GFP Ed Boyden AV-1-ALL864 51503-AAV1 AAV pCAG-FLEX-tdTomato-WPRE... 51503-AAV9 AAV pCAG-FLEX-tdTomato-WPRE Hongkui Zeng AV-5-PV2509 29777-AAV5 pAAV-CAG-ArchT-GFP Ed Boyden...

...Cre-dependent GFP expression was detected with direct fluorescence. GFP was not detected in the absence of Cre. mCherry...[Jaws-KGC-GFP-ER2] (Addgene 84445-AAVrg) alone at 1.1E6 vg/mL, or both. Two days later, Cre-dependent ... and quality control are described below. AAV Production AAV distributed by Addgene has been produced ...purity of the AAV preparation. Figure 1: Silver staining of purified and non-purified AAV subjected to ...images). Figure 2: AAV Pro cells were transduced with either pAAV-Ef1a-mCherry-IRES-Cre (55634-AAVrg) alone... prep # 55632-AAVrg). pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] was a gift from Edward Boyden (Addgene viral...using our virus protocols . Browse our AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral...

...pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre recombinase. Floris ...Eric Kowarz 96930 XLone-GFP Tet-On PiggyBac vector for inducble expression of EGFP Tet-On 3G rtTA TRE3GS...171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector for inducible expression of EGFP Tet-On 3G rtTA TRE3GS...pTet-IRES-EGFP Lentiviral plasmid for Tet-controlled expression of transgene of interest with EGFP (On or... pK170.AAV-TRE-Cre-WPRE (Supernova) AAV vector for dox-inducible expression of Cre recombinase. Takuji... Off) None TRE, miniCMV Maria Lung 16542 pBI-MCS-EGFP Bidirectional promoter (Pbi) for Tet-responsive ...expression (On or Off) of both your gene of interest and EGFP. Pbi contains a TRE between two minimal CMV promoters...

...Fluorescent proteins (GFP, mCherry, etc) Localization pcDNA3-EGFP - C-terminal GFP for mammalian expression...expression vector GFP Varies pMKO.1 GFP - Retroviral shRNA expression pLenti CMV GFP DEST - ...blue fluorescent protein 35S-eGFP-nosT - Transient expression vector for eGFP in plants Find FP tagging ...genome MSCV-IRES-GFP or pMSCV-IRES-mCherry FP - Mammalian retroviral gene expression with GFP or mCherry selectable...Lentiviral shRNA expression pLJM1-EGFP - 3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin Hygromycin...Expresses mCherry tag in mammalian cells pBI-MCS-EGFP - Tet-inducible Find more Tet-inducible empty backbones...fusing your protein to another protein, such as GFP, which allows you to visualize the cellular localization...

...Calcium erGAP3 (GFP-Aequorin Protein) for imaging of Ca 2+ dynamics in endoplasmic reticulum GFP-Aequorin Protein...FlincG3 (GFP-based cGMP sensor) for imaging in C. elegans neurons Using a Robust and Sensitive GFP-Based ... jGCaMP7 High-performance GFP-based calcium indicators (Constitutive or Cre-dependent) High-performance.... We also offer ready-to-use AAV preparations of select plasmids and AAV Packaged on Request for many ...cyclic AMP) Signaling reporter island (SiRI) with GFP-based fluorescent reporter cAMPr Spatial Multiplexing... FLAMP Plasmids Jun Chu cGMP (cyclic GMP) FlincG GFP-based cGMP sensor Differential patterning of cGMP... vascular smooth muscle cells revealed by single GFP-linked biosensors. Proc Natl Acad Sci U S A. 2008...

...pAAV.CMV.Luc.IRES.EGFP.SV40 Firefly CMV AAV expression of firefly luciferase and GFP James Wilson 22522 phGluc Gaussia...-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla EF1α AAV-CRISPR knockout plasmid. Renilla ...Gaussia EF1α Expression of Gaussia luciferase; GFP is expressed if cells are infected with virus Christopher... with a fluorophore as the acceptor. LumiFluors: EGFP-NanoLuc ( GpNLuc ) and LSSmOrange-NanoLuc ( OgNLuc...extracellular pH sensor consisting of a pH-sensitive GFP (superecliptic pHluorin), a pH-stable OFP (Antares...expressing luciferase. We also offer ready-to-use AAV preparations of select luciferase expression plasmids...Stephen Tapscott 83281 pAAV-CAG-FLuc Firefly CAG AAV expression of firefly luciferase Mark Kay 105532 ...

..., Hess et al. successfully evolved wild type GFP to EGFP using CRISPR-X and subsequent FACS sorting. They...with unique tissue-specific promoters expressing GFP, tetracycline response elements, and shRNAs many ... of DsRed connected to a pH-sensitive variant of GFP (SEP) by a 9 amino-acid linker (see Figure 1). The...pAAV-CMV-DIO-Magneto2.0-sNRPpA AAV 74306 pAAV-CMV-DIO-TRPV4-p2A-ferritin-sNRPpA AAV 74302 pDestTol2CG2-Neurog1...loxP-stop-loxP cassette into the Rosa26 locus, allowing for Cre-dependent expression of a GMAP inserted gene. The...plasmids, which already includes dSaCas9-VPR in an AAV transfer vector for in-vivo expression and plasmids...Magneto2.0 in the mammalian brain after delivering it via AAV injection, finding that it could increase the activity...

...pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest...for replication. For more information about AAVs, read our AAV guide . Recombinant Adenoviral Vectors Wild-type...throughout viral vector production. During experiments, GFP can be used to sort cells infected with rAdV vectors...recombinant adenoviral vector production that contain IRES-GFP. Pseudotyping The production of viral vectors (or...adenoviral vector production that do not contain GFP. Recombinant adenoviral vector (rAdV) Modified form...Browse Adenoviral Plasmids Adenovirus versus Adeno-Associated Virus Adenoviruses fall under the Adenoviridae...double-stranded DNA genome. Adeno-associated viruses (AAV), however, are small single-stranded DNA viruses ...