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We narrowed to 208 results for: pme i

Showing: 1 - 50 of 208 results

  1. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...complex) Class 2 (Single multi-domain effector) Type I (Cas3) Type II (Cas9) Type III (Cas10) Type V (Cpf1...5429-33. PMID: 3316184 Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. 2012. A programmable...26422227 Zetsche B, Heidenreich M, Mohanraju P, Fedorova I, Kneppers J, DeGennaro EM, Winblad N, Choudhury SR...An overview of CRISPR's development from a prokaryotic immune system to a valuable genome engineering... CRISPR CRISPR History CRISPR History and Development for Genome Engineering You may also like... CRISPR...multiplexibility in various species, as well as the development of new applications. The first CRISPR papers ...CRISPR Technological Advances CRISPR technology development has occurred at a truly astonishing pace, with...

  2. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type
    Blog Post
    ...University of Iowa. “I left not really knowing what I wanted to do, but knowing that I wanted to leave the...and not know anyone. I say I’m the anomaly because I didn’t network to get to where I was. It was really...for Caitlin Runne-Janczy. “I wrote reports for fun in fourth grade because I was weird like that,” says...rather at the beginning. “When I was at Iowa in the Department of Pharmacology, I taught a couple of lectures...the faculty members, I helped grad students who were teaching for the first time. I walk[ed them] through...here, I was still editing from home and looking for a job.” But Caitlin’s persistence paid off: “I spent...love of teaching also runs through Caitlin’s blood. “I come from a family of educators, mostly elementary...

  3. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    ...Facebook. “I think this helped me say with confidence when I interviewed, ‘I write science. I edit science... an SME: “I write curriculum for students taking science courses online, which is a blast. I have so much...her first post-grad school job, head over to part I of the interview. Caitlin Runne-Janczy’s first day...at eScience Labs was not your typical first day. “I walked in and they asked me, 'How do you feel about...for] electrophoresis or doing a dissection video. I did that in January. This involves going in front ...responsibilities.“I’d say maybe 25% of my day is still what I did as an SME, and then 75% of the time I’m kind of...science. And I do it well.’” Stay tuned for the next feature in this series. We’ll be interviewing Susan...

  4. Immunology Research Plasmids and Resources

    Type
    Collection
    ...histocompatibility complex, class I, A FLJ26655, HLAA HLA-B major histocompatibility complex, class I, B AS, HLA-B-7301...complex, class I, F CDA12, HLA-5.4, HLA-CDA12, HLAF HLA-G major histocompatibility complex, class I, G MHC-G... MHC class I polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related...SKR3, TSR-I ADCYAP1R1 adenylate cyclase activating polypeptide 1 (pituitary) receptor type I PAC1, PACAPR...Antigen presentation is mediated by the MHC class I and class II molecules found on the surface of APC...

  5. Protocol - Bacterial Transformation

    Type
    Protocol
    ...Incubate plates at 37°C overnight. Tips and FAQ How can I save time when carrying out transformations? If you...is a good idea to outgrow for at least 20-30 mins) I get very few if any transformants when transforming...transforming large plasmids (>10 kb) or BACs, what can I do? Chemically competent cells are fast and easy to use... Follow the manufacturer's instructions for each. I got no transformants. What went wrong? Check that ...learn how to isolate single bacterial colonies. Equipment Shaking incubator at 37 °C Stationary incubator...

  6. COVID-19 Resources

    Type
    Collection
    ...Severe Acute Respiratory Syndrome Coronavirus Class I Fusion Protein Upstream of Rather than Adjacent to...Beating Affected by the Pcp4 Dose-Dependent [Ca 2+] i Increase in Down Syndrome Mice, Ts1Rhr . Do you have...Nanobodies now available from Ray Owens' lab. Development of CRISPR as an Antiviral Strategy to Combat ...relevant to SARS-CoV-2 Research Coronavirus Method Development Community at Protocols.io Addgene's Protocol ...

  7. Making Career Development an Integral Part of the Science Training System

    Type
    Blog Post
    ...and integral part of all science training programs. I believe in the academic apprenticeship system as a...PhD Career Development; a Stakeholder Workshop Report.” The report enabled the development of priorities...their training.   Figure 1: Professional Development Hub Workshop Attendees and their 2021 report....the structure and know-how to integrate career development in science training programs. In 2018, the ASBMB...level with the creation of the The Professional Development Hub (pd|hub). Pd|hub was spearheaded by Cynthia...committed to advancing career and professional development as core components of predoctoral and postdoctoral...General Medical Sciences (NIGMS) will support the development and implementation of collections of evidence-based...

  8. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...Solution I - Resuspension Buffer 25 mM Tris-HCl (pH 8) 50 mM glucose 10 mM EDTA Store Solution I at 4°C .... Resuspend the pellet in 100 μL of cold Solution I. Vortex the solution for 2 min or until all bacteria...bottom of this page. Last Update: Feb. 8, 2018 Equipment Desktop microcentrifuge Desktop vortexer Vacuum...

  9. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Zalocusky KA, Bernstein H, Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve R, Huang ZJ, Deisseroth K...59. PubMed (Link opens in a new window) Lazaridis I, Tzortzi O, Weglage M, Märtin A, Xuan Y, Parent M,...INTRSECT Implementation Plasmids References The development of powerful molecular reagents for biology have...

  10. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ...example (which I will come back to) is the one-eyed pinhead mutant, which regulates a developmental pathway ..., Christiane. "The zebrafish issue of Development." Development 139.22 (2012): 4099-4103. Additional resources..., embryo growth, brain function, and disease development. Why have zebrafish become such a popular model...resources and highlight two recent breakthroughs in developmental biology that arose from zebrafish-based investigations...the gene networks that regulate processes from development to metabolism to fertility. Several zebrafish-specific... These mutants are used extensively to study development and disease. Additional mutagenesis projects ...affecting diverse biological processes—not just development—are available from strain distribution centers...

  11. CRISPR Library Amplification

    Type
    Protocol
    ...Pooled Libraries Molecular Biology Reference How do I process my Addgene pooled library? Introduction Please...total plasmid DNA. Tips and Troubleshooting What do I do if my transformation efficiency is not high enough...light in the cuvette during the electroporation step. I got a heavy recombined band by digest. Recombination...ensure that outgrowth and competition are limited. Equipment Table top centrifuge BioRad Electroporator (MicroPulser...and follow all specifications described in the equipment manual. Immediately add 1 mL SOC to cuvette. Remove...

  12. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Michael Davidson Collection Blog: Which FP Should I Use? Blog: How to Choose a FP For Multi-Color Imaging...-C Cloning Vector - Mammalian Expression mScarlet-I 569 593 57 5.4 36 min Monomer pmScarlet-i_C1 - Mammalian...Bacterial Expression mEGFP 488 507 34 6 Monomer (A206K) pmEGFP-1 - Mammalian Expression mEGFP-N1 - Mammalian Expression...18.4(blue), 5.8(red) 2.7/4.7 3.9 hr (red) Monomer pMedium-FT-N1 - Mammalian Expression pTRE-Medium-FT - Mammalian...

  13. Genetic Code Expansion

    Type
    Collection
    ...AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA) 1R26PylRS and AfTyrRS CbzK and p-I-Phe Bacterial... 200225 pMega-MaPylRS PylRS M. alvus Nε-Boc-lysine Bacterial TAG Alanna Schepartz 200226 pMega-MaFRSA ...

  14. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...linearized. Treat the purified AAV samples with DNase I to eliminate any contaminating plasmid DNA carried...Haase M, Muether N, Hausl M, Rauschhuber C, Huber I, Nitschko H, Busch U, Sing A, Ehrhardt A, Baiker A...set-up: 2 h qPCR run: 1.5 h Data analysis: 30 min Equipment qPCR instrument Heating plate Pipettors 1–10 µL...

  15. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...binding domain, Type I/II) [N120A/9.1R] Neuregulin-HBD (Heparin binding domain, Type I/II) Human Mouse IgG2a...177570 Anti-mGluR1/5 (Group I) glutamate receptor [N75/33R] mGluR1/5 (Group I) glutamate receptor Rat Mouse...182119 Anti-mGluR1/5 (Group I) glutamate receptor [N75/3R] mGluR1/5 (Group I) glutamate receptor Rat Mouse... M. musculus (mouse) IgG2b 227029 mGluR1/5 (group I) glutamate receptor (Rattus norvegicus) recombinant...recombinant mouse monoclonal antibody. mGluR1/5 (group I) glutamate receptor Rat M. musculus (mouse) IgG2b 227030... new window) Gavira-O'Neill CE, et al. 2020. Development, Screening, and Validation of Camelid-Derived...

  17. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...said, “Boy, I love science,” and I realized, I still do too! Beyond the outreach experience I gained from...t something I liked – well, I was used to experiments not working, so just like in lab, I sucked it up...moved on to the next thing. If I made a mistake, I evaluated what lessons I had learned – call it “troubleshooting...as a graduate student, and I love it. I am able to write about any topic I like and gain experience in...students because I wasn’t sure what I wanted to do. A few weeks into volunteering, I was talking to a ...your professional development I had a wonderfully supportive mentor and graduate office. I cannot stress ...four years into my PhD in Molecular Biology, I realized I wanted a career in science outreach: engaging...

  18. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ... find it useful, I highly recommend doing an AMA! Before I turn it over to Joanne, I thought I’d share...relationships. I also like Twitter because I can get a little opinionated and, while it can be anonymous, I can ... to blogs to recipes. I am not paid to say this, but I really don’t know what I would do without it. So...social media, I must say that doing a Reddit “Ask Me Anything” was appealing, but also scary. I do a lot of... public speaking where I enjoy communicating face to face with the audience. I post on LinkedIn where ...It seemed like I would really have to open myself up to anything for a Reddit AMA and I didn’t know what...even despair at having chosen an academic past. I hope I was able to provide some good resources to the...

  19. Finding Your Perfect Job After University

    Type
    Blog Post
    ...positions.  I had to reassess what I wanted to do and so I applied for a role in Industry. I wanted to ...to gain relevant experience. I applied for this summer position as I felt I needed more practical lab experience...wasn’t a good fit for me, then I didn’t need to explain why I changed jobs. I highly recommend taking on ...ended, I wanted to explore another area which I was really passionate about during my degree. I loved ... completing my MSc, I again had to look at what type of work I would like to do. I had taken advanced ... job, but I knew that I enjoy helping people and decided to compromise and applied anyway. I later found...different sectors. Luckily, I have worked for a variety of different organizations, so I tend to provide them...

  20. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...afield at first and I let them pass by. But I didn’t forget: what would it be like? Then I spotted an opening...one I just could not resist applying for. Not that I knew anything about being an editor - that I learned...manuscripts. I work with people, nurturing networks, as much as with technical knowledge. I leverage a ...egg is packed with information. I am fascinated with how developmental programs unfold, absorbing the ...down into a singular focus on molecular pathways, I found myself curious about other routes – In terms...fascinating biology is found in weird and rare organisms so I found it hard to stick to vertebrates alone.  And ...inclination to be a teacher was looking for an outlet. I started asking people: why do you do what you do? ...

  21. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...second postdoc, I realized that working in the wet lab wasn’t the right path for me. I began exploring... transition wasn’t easy - I had to reinvent myself and redefine the value I could bring to potential employers... employers. Over time, I discovered that I not only enjoyed the technical aspects of my work but also ...below. Common pitfalls in the transition (what I wish I knew before experimenting on myself) Assuming...structure. I quickly learned that my approach needed to adapt based on individual needs. For instance, I had ...small-scale opportunities where I could make mistakes and learn from them. I started at my previous company...role. Along the way, I discovered strategies to effectively pivot my career development and align it with...

  22. Transferable Skills: Negotiation

    Type
    Blog Post
    ... as I applied for jobs, I knew I should negotiate my salary after receiving an offer but even as I did...What I didn’t realize was how much I was constantly negotiating, every day. As a grad student, I was negotiating...Negotiation. As a grad student, I thought that “negotiation” was something that I would mainly do over the price...process felt unnatural and intimidating. I felt unprepared. Mainly, I thought of negotiation as something ...negotiation as a zero sum game - that is, if I get what I want, that means you don’t. In this mindset,...solutions that benefit everyone. Even if I “lose” a negotiation, I will view the “winner” positively if they...take my vacation? I want to learn more about a different area of the company. How can I negotiate with my...

  23. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...media as a scientist. I mean, I had a LinkedIn page and a Twitter handle, but I wasn’t using them to my ... blog. I wrote my first blog post out of boredom. I had just started working in a new lab and I was the...When I saw a request for an Addgene guest blogger post about colony PCR, I signed up immediately. I had...Before I started writing for the Addgene blog, sharing Chemistry Cat memes was how I used social media...Twitter that I realized 1) a lot of scientists are using social media professionally, and 2) I needed to ...Chemistry Cat memes use to be how I used social media as a scientist. Now I write for the @Addgene blog & ... maybe hadn’t used this method. I enjoyed writing the first post so I signed up to write a second. While...

  24. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    ...Addgene. So, when I received an invitation to join the Advisory Board of Addgene, I accepted the offer...offer without any hesitation. I hope I will be able to effectively promote Addgene, which is also beneficial... Basel, I felt that the time flows much slower, like the flow of the Rhine River. In the US, I very much... the other hand, I feel that researchers in Japan are not eager to interact, which I don't think is good...was done in Japan, which is very disappointing. I hope I can help change the situation in the future. Addgene...the rules of the game. In this regard, I believe that development of new technologies may be very effective...such as TAL proteins and CRISPR became available, I was very excited because genome-editing with these...

  25. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...the media.  At one point I started to become very aware of how little care I took with my discards and...and to be mindful of the lab materials I was using.  I started to do little things to save reagents and...glass powder in 50% nitric acid, but I never let that stop me. I only had to replenish my glass milk supplies...supplies every 10 years or so. Actually, I was happy to hear that I am not the only Addgenie who has a thrifty...My other stingy science project started when I realized I was pouring large agarose gels for DNA visualization...gel.  What a waste?  I started looking for smaller and smaller gel apparatus until I settled on using glass...taking conservation to new levels, I was bothered by the fact that I was wasting buffer running my tiny...

  26. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...When I first began collaborating with people outside my lab’s department, I personally felt that I had ...conference attendance, and I scout for potential business partners. I am glad that I was able to build experience...professional updates. Whenever I see interesting events, articles and newsletters I make sure to share them ... Addgene I work with people from various organizations in science and beyond. For example, I help organize...department where we shared materials and equipment between labs. Later I worked with people outside the department...your comfort zone As part of my position at Addgene I am now involved in Strategic Partnership Management...During my graduate studies at Karolinska Institutet I started working with other groups. First within my...

  27. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    ... paper, I usually try to grasp the main message of the work. I underscore the sentences that I found hard...comments to “you” I can easily check if I would have formulated specific comment differently if I would be speaking...After finishing my section-specific comments, I re-read what I wrote about the paper after my initial read...Therefore, I try to stay away from the loaded and judgemental language. In each peer-review, I try to mention...postdoctoral researcher studying plant development and abiotic stress tolerance, I have been peer-reviewing journal...to be replicated or omission from the literature. I think that delivering constructive critique can help... 2015 for a wide variety of journals. Since then, I developed a routine that works well for me to evaluate...

  28. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...leave. I really enjoyed my time in the lab, my colleagues and what I was researching, but I decided I needed...just as important. The problem was I knew I had a knowledge gap. I had no previous business, marketing...marketing or sales training and knew I had to start somewhere. I knew if I started with the Harvard Business ...of researchers. For many years I struggled with the questions of “Will I leave academia?” and "What type... would I do next and what options lie ahead of me? Like a considerable number of researchers, I had high...business of western blots and cloning, I decided to pack it in. I had just started month 8 of my 3 year... for the job To become an attractive candidate I knew I had to push not only my academic background but...

  29. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...half into my role, I realized that actually, I didn’t want to go to grad school. But, I didn’t know what...ago when I started my career transition from bench science to software product management, I confess that...management? I always tell people the best part of my job is getting to talk to so many different people! I communicate...project I led. It’s divided into the key takeaway themes and customer pain points we’ve identified. I can’... that I had no idea what software product management was either. Coming from a strong background of undergraduate...research straight to a lab career at a biotech company, I always assumed I’d work one or two years at the company... and begin my product management (PM) career. Had I known about product management earlier on in my career...

  30. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

    Type
    Blog Post
    ...to Addgene? So why did I join Addgene? Was I jaded by my time in academia? Did I run screaming from the...things to people. When I started my graduate work at Harvard, I recognized that I would ultimately want... the right?!? I would love to tell such a dramatic story, but it wouldn’t be my story. I love academics... and here); however, simply put, I moved into this position because I want to help make the research enterprise...friends. In my teenage years I was super shy - there was even a time when I was too nervous to ask store...help at the mall. In undergrad I was forced to open up a little after I dropped myself into Boston University...research at BU, I realized that I’m energized by talking to people about science and that I love explaining...

  31. How to Write a Scientific Review Article

    Type
    Blog Post
    ...references. I cite as I write, meaning I use the software to add the citations in real time as I write. Things...hits. So, here, I will share my process with you. But before I do that, let me say that I was by no means...peers. Start reading! I started by reading other reviews because, as I mentioned, I wasn’t an expert in ...cared about. I also used reviews to get a list of research papers that I needed to read. Once I had an idea... of authors. Just start writing When I first started I thought I would read a bunch of papers and then... or anything else. Like I mentioned, I also added citations in real time as I wrote, so each statement...initially I felt that I needed a huge chunk of time to sit down and get anything done, so unless I had at...

  32. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...very, very quiet. Every time I asked a question, I felt like I was yelling. I imagined 6 years of biting...all. Finally I rotated in a small lab with a new PI. I realized that as a first born child, I would thrive...later. I hear too many science trainees get warnings and still think “but that won’t happen to me, I have...work and talent for many years (at a very low salary I might add). You have a right and responsibility to...talking about simple things like “Which route should I take to get to work?”; we often make important life...where we will spend the next 6 years. Know yourself I rotated in 3 labs before choosing a graduate school...second rotation was in a big lab with a famous PI who I was only likely to see once a year. There was lots...

  33. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ...their career path. Graduate school: Do I or don’t I? I asked the nine Addgenies about why they did...master’s and leave. I was really devastated when that happened. I didn't know if I should even stay in...Control team, I help make sure the plasmids that go out our doors are correct. Now that I am heading into...pretty easy choice for me,” she said. “I had always been a student and I truly enjoyed learning.” Addgene’s...the thing I wanted more of [after undergrad] was to learn and speak the language of science. I didn’t come...not go. She told me, “I think that my experience is more valuable at this point. I don't know where a master...full time as a Lab Technician. “I was so tired of being in school so I just wanted to work,” she said....

  34. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...tasks and I made a point of sticking to deadlines and performing the work I agreed upon (just as I would ...Members. In some instances I felt like I was going back to previous formats but I learnt to overcome the ...people at that conference, I later became involved with the NPA beyond the meeting. I first joined a committee...committee member, I volunteered for various tasks as things came up. For example, I updated some sections...implemented and I had to adapt accordingly. For example, the quarterly and annual reports I had to write ...for the organization as a whole. I came to understand that not only was I growing as a leader but the organization...leader in the NPA, I had to speak as an NPA representative and speak the NPA’s stance. I had to be professional...

  35. Zhang Lab CRISPR Page

    Type
    Collection
    ...Delivery AAV - Cas9 Mouse CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is a microbial...SpCas9, driven by the truncated MeCP2 promoter (pAAV-pMecp2-SpCas9-spA) for expressing Cas9 in neurons. SpCas9...

  36. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...employer, I took on my first consulting job, and I began to realize I too had a niche. As a result, I started...infrastructure. My career path changed when I was asked if I would consider consulting for a particular...program changes, I learned to write grants for health, housing, economic development, education, workforce... How did you get started in the grant profession? I asked Deborah Cook, Ph.D., GPC, previously a college... to scientifically oriented nonprofits. Like Deb, I worked for an organization in a position that required...process and managing programs that were grant funded. I worked for a health department and then for the regulatory...and then our grant training and mentoring. How do I become a grant professional? Employment opportunities...

  37. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...similar development stage, they have a lot to learn from each other. Some have taken my advice and I recently...the Addgene Blog Mentoring for Scientists Series. I have been thinking a lot about Mentoring for over ... these elusive teachers, supporters and advisors? I tried to start a more formal mentoring program at ...and be matched with the many interested mentees. So I experimented with a group mentoring format where 1...her mentoring style. While working on this project, I read the book “Every Other Thursday: Stories and Strategies... the group mentoring format could work wonders.  I have now seen the formation of hundreds of mentoring...mentoring program at your organization, contact me! I can provide a step-by-step, how-to guide to get one...

  38. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    ...of experience. I am away from the bench now, so I have to talk to other scientists when I am in new territory...for selfish reasons: I wanted to learn about careers in science communication. When I started my Science...looking for someone who might take over. I like writing and science, so I took a chance.” It’s been six years...job. “I am really more of a multimedia science communicator than a science writer these days. I do some...’ workload. “I am currently getting abstracts and plans together for my next 4 webinars—I have them stacked... it, and be flexible. The best stuff that I do is not what I was hired to do.” Get Networking Tips Here...Science Communication Internship with Addgene, I didn’t know a lot about scicomm, but had enjoyed writing a...

  39. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    .... Last time I checked we were ranked as the 21,806th most popular website in the world. I know, right?..., low meeting, and low email culture. I like working here and I hear from the Addgenies that they do too...but definitely not worried. I have confidence that from Business Development to Shipping we will execute...increasingly seeking us out on their own to share reagents. I love the way scientists starting their own labs get...as much a tech company as it is a reagent company. I used to have to worry about this a lot, but not any...online for Addgenies and to serve external customers. I also can’t wait to see where our new data initiatives...continue to incorporate additional Board best practices. I thank them wholeheartedly for their service and support...

  40. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...seriously. As I make my rounds speaking at research institutions and at scientific conferences, I see that ...coaches? Yes I can. Please note that Addgene does not endorse any of these coaches and I can't tell you...it.  Because I am located in Cambridge, MA (and because we have a lot of scientists here) I know many coaches...speaking engagements as are many of the coaches I have mentioned. I have seen a lot of fantastic online content...will make you take the experience more seriously and I have seen, without exception, that this helps people...reaching their goals.  Check out Joanne's Reddit AMA I am a strong advocate for peer mentoring, but volunteer...will cost a fortune for a successful outcome. How do I find a coach? Start with referrals. Are there scientists...

  41. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ..., or so I thought. So I entered a molecular and cellular biology PhD program in 2011, which I left four...I didn’t plan to get a master’s degree. I wanted to be a scientist and you need a PhD to be a scientist...exactly the type of scientist I wanted to be. But prior to graduate school, I wasn’t sure a master’s was ...more autonomy?  Before my master’s, I worked in an academic lab where I ran a handful of assays over and ...and over. Everyday was the same and I was bored. I wanted more variety and more power to influence the direction...still decide to earn a PhD, but if I had to do grad school over, I would just do the master’s! If you'...degree felt like a consolation prize, but in time I realized its value. My master’s strengthened my critical...

  42. Career Insights: Technical Support Specialist

    Type
    Blog Post
    ...unpleasant experience I thought: I could do this job so much better. Driven by that, I successfully applied...junior postdoc I was aware that such roles existed, but did not consider them for myself. I started picturing...biotech companies), and as far as I remember, it was the first time that I was absolutely convinced it was...company’s fault. I contacted them to express my concerns and request a replacement. I got my replacement...picturing myself in a technical support role only when I was confronted one day with a product issue in the...resolved - but it was a rather painful to get there: I had to send the company detailed documentation of ...of my results, answer loads of questions, and I also had to do some troubleshooting as advised by the technical...

  43. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...health. As a teenager in high school, I knew that this is what I wanted to do,” Stegmeier says He began...treatment of rare and ultra-rare diseases. “Today, I am a scientist that helps with different aspects of...Baradaran’s path to science began in high school. “I had a great chemistry teacher in 10th grade and then... in for a long time. “At an early age, I was fascinated by the impact that medicines can have on human...foray into genetics during graduate school at MIT. “I read some of the seminal papers on cell cycle discovery...the University of Leuven in Belgium. “That’s where I not only got incredibly compelled by wet bench molecular...disrupt these inefficiencies is pretty remarkable. I was compelled to be part of that.” He’s deposited ...

  44. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...listening to me. When I got home, I saw my daughters playing with some cards and I had a quick flashback...engagement. I figured, an engaging card game could help me get the engagement I wanted. I therefore started...some other professors, I went home thinking about how to solve this problem. I didn’t want to face another...diseases. That’s when I thought, if it cannot be hands-on, it will be “brains-on”! I know that even lectures...as much as I try being compelling, interactive, and application-driven to boost curiosity, I have never...courses about gene therapy and genetic manipulation, I and other professors at the University of Barcelona...you might have to drastically alter your strategy. I realized that similar types of thinking go into developing...

  45. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...Pharmaceuticals in Cambridge, MA. When I joined Sanofi/Genzyme nearly three years ago I decided it was time to get...kids, I had put on typical middle age weight and “exercise” consisted of taking walks in the mall. I took...After being with the company for about six months, I learned there was a Genzyme-based running team called...correct treatments, if available. As part of the team, I started adding mile after mile on my poor, poor legs...partners so that they can help tell their stories. I was paired with a six-year-old boy named AJ Gonzales... bone that only further impairs the patient. When I first met AJ and his family, he did not seem much ...supporting AJ, who is a big superhero fan. This year, I have decided to run as The Green Lantern’s friend,...

  46. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...USA). I had never participated in a protest before, so I had no idea what to expect. Admittedly, I was ...Below I present perspectives on the March for Science from researchers all over North America. I hope ...funding.  So when I saw that there was going to be a march in support of science, I loved the idea and...38.9072°N 77.0369°W – Mark Lucera I am a passionate scientist.  I care about my work because it's important...86.7816°W- Jennifer Stancill I am a research biologist by training, so of course I felt compelled to participate... hostile anti-science activists. However, I must say that I was supremely proud of Nashville that morning...37.9101°N 122.0652°W – Steph Hays When I marched for science in May, I marched to advocate for the process...

  47. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...extremely helpful, but with hindsight I wished I had gotten them from Addgene. I really struggled to get those...information and I believe that a lot of European students and researchers are craving, like I was, for a better...share their plasmids. Now I am on the other side of the force and as an Addgenie I will do all my best to...to help them in their tasks. But I won’t be alone. I join another Addgenie in our office in London, Emma...PhD in Developmental Physiology at the University of Paris VII. During my years in research, I developed...service: Addgene’s London office Just a few weeks ago I became the newest Addgene Outreach Scientist - the...supporting European scientists. Born a french frog, I moved to Albion six years ago to work as a research...

  48. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...of which I could identify. There were dozens of different mushroom varieties alone. At least I could tell...mushrooms. In some cases I couldn’t tell if the bags contained flora or fauna. I tried to find out what ...Having never been to China myself, I was pleased to be able to participate when the organizers of the...to hear current findings in nucleic acid science. I was delighted to see that the majority of attendees...companies and start-ups choose to locate there. I made time to go into the city of Guangzhou and see some of ...overlooked regulatory fragments of these transcripts. I remember when small RNA was just something we ran ... Rajewsky lab is studying circular RNAs (who knew…I had barely heard of these). These single stranded,...

  49. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ... of 2014, I began developing software to make sgRNA design accessible to all. At the time I thought there...focus further development toward. Quality control is, however, not a focus of this blog post. I separate sgRNA...CRISPR software and the piñata effect Two years ago I was a part of a group (Biology of Host-parasite Interactions...Plasmodium falciparum (the parasite that causes malaria). I attribute their success to technical expertise, thoughtful...software to use. With so much choice the question I have is, how did we get here in the first place? How...time to assess public response; and, it is the stage I think we are currently at in the CRISPR software space...to succeed. With respect to de novo sgRNA design, I again separate it into two categories. On the one ...

  50. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ...getting tighter and competition is fierce. However, I believe there are plenty of great jobs out there for...conference is organized by postdocs for postdocs. I am delighted to see enterprising early career scientists...working together on the event planning and execution. I am seeing many similar examples of science trainees...Penn Biotech Group and PBG Healthcare Consulting. I know of many schools that have completely student/...career preparedness training for their scientists. I have worked with some great postdoc and grad student...own hands, there are some easy ways to get started. I am happy to work with any group of trainees interested... creating their own opportunities for career development and networking. Aside from the importance of ...
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