We narrowed to 343 results for: ATC

...44249 pdCas9-bacteria pTetO Anhydrotetracycline (aTc) Escherichia coli Stanley Qi 11518 pDest-527 T7-lacO...Moeglich 68940 pRMC2 Pxyl/TetO Anhydrotetracycline (aTc) Staphylococcus aureus Tim Foster 36267 pBAD33.1 ... Lu 17972 pSE100 Pmyc1/TetO Anhydrotetracycline (aTc) Escherichia coli , Mycobacterium tuberculosis Sabine...Ehrt 44561 pST-KT Pmyc1/TetO Anhydrotetracycline (aTc) Escherichia coli , Mycobacterium tuberculosis Vinay...Christopher Marx 44448 pLC291 pR/TetO Anhydrotetracycline (aTc) Methylobacterium extorquens Christopher Marx 122635... Peters 127088 pMS17 tcp830 Anhydrotetracycline (aTc) Streptomyces sp. Maggie Smith 74065 pZ8-Prp PprpD2...

...pLC97 plasmid allows for quick integration of the aTc-inducible pTet-dCas9 in the attachment site of the... or 20 matching nucleotides, the second binds to mcherry with either 0, 11, 18, or 20 matching nucleotides...thus strongly decreasing the expression level. Mismatches between the guide RNA and the target sequence...repression level. By controlling the number of mismatches we can obtain a range of expression levels. This...

...efforts is the Golden Stopwatch Award to celebrate exemplary TTOs. Our new Golden Stopwatch Award recognizes...agreement (usually) must be executed. The Golden Stopwatch Award highlights TTOs that accelerate science ...agreements quickly! If your TTO receives a Golden Stopwatch, consider sending an email to thank them--after...examples if possible Tell them about the Golden Stopwatch! Any organization that deposits with and/or [email protected] Congratulations to the Golden Stopwatch awardees! Congratulations to all of the winners...

...functions. Many tools have also opted to mix-and-match different scoring algorithms and parameters. This...that make each tool unique. The CRISPR Software Matchmaker is composed of these features and enables you...multi-target design”, “off-target aware”, “high mismatch limit”, “approximate design”, “empirical design.... Trends: The most common utility functions are batch design or multiplex features. However, tools aiding...at the glossary section of the CRISPR Software Matchmaker, it provides a list of terms I think are important...going shopping for one. Use the CRISPR Software Matchmaker to select the best tool based on your needs. ...on How to Design a gRNA Read Our CRISPR Guide to Catch up on All the CRISPR Basics Read Other Blog Posts...

...BxbI gene downstream of the anhydrotetracycline (ATc)-inducible promoter (PLtetO), the TP901 gene downstream...efficiently do RSMs work? We tested a two-input (ATc and Ara) RSM over a time series of increasing input...

... multicopy integration. (B) Anhydrotetracycline (aTc) induces expression transposases leading to increased...

... the inducer, in this case anhydro-tetracycline (aTc). By cloning in two tandem copies of the gRNA cassette...recombineering, the locus of interest is modified to match the repair template, and the locus cannot be recognized...

...OPEN gAGCATCACCtcgctGGAGGTGAGc PTRF/Cavin1 OZ513 and OZ514 OPEN OPEN gACAGCCGTCtgcatctGGAGGTGCCc pitrm1... OPEN OPEN aTCCATCCCAaacacatGTGGCAGAAt NFATc1 OZ543 and OZ544 OPEN OPEN aCCTCGCCTCagtgtGACGGAGGAc Swachmann-Bodian-Diamond...CODA CODA gCTCATCTCCACCGAGCTGGAGAAg atp2c1 OZ567 and OZ568 CODA CODA tTACCTCCACGATCTTGCTGATGGAg dio2 OZ569...hydroxylase (dbh) OZ525 and OZ526 OPEN OPEN tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527...gpcr-2037) OZ535 and OZ536 OPEN OPEN aGCCATCATCaggttcaGGATGAGCCc Kif6 OZ537 and OZ538 OPEN OPEN aAGCTTCCTCttcattgGCTGACTGCa...lipoprotein receptor) OZ539 and OZ540 OPEN OPEN tTGCATCCACggcagTCGGCAGTGc Encephalopsin (Opn3) OZ541 and OZ542 OPEN...gCGCCACGGAcaataaaGAGGACTGCa GUSB OZ551 and OZ552 OPEN OPEN gGTCATCTGCcattcGAGGCGGACa REST corepressor 2 OZ553 and OZ554 OPEN...

... calcineurin-dependent 1 MGC138448, NF-ATC, NFAT2, NFATc NFATC2 nuclear factor of activated T-cells, cytoplasmic... NFATX NFATC4 nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 NF-ATc4, NFAT3... KIAA0827, NF-AT5, NFATL1, NFATZ, OREBP, TONEBP NFATC1 nuclear factor of activated T-cells, cytoplasmic...

...direction. The location of any potential mismatches matters. Mismatches between the target sequence in the ...target DNA. If the seed and target DNA sequences match, the gRNA will continue to anneal to the target ... seed sequence inhibit target cleavage, while mismatches toward the 5′ end distal to the PAM often permit...HDR) pathway. HDR relies on copying DNA from a matching template to accurately repair or fill in the missing...contains the desired edit as well as additional DNA matching the sequence immediately upstream and downstream...resulting heteroduplex is resolved by the cell’s mismatch repair system. Alternatively, the edited 3’ flap... sgRNA directs Cas9n to a nearby site, driving mismatch repair using the edited strand as a template PE4...

... 21) Prefix: GAATTC ATG AGATCT Prefix Enzymes: EcoR1 , BglII Suffix: T GGATCC TAA CTCGAG Suffix Enzymes...Enzymes: BamHI , Xhol Scar: GGATCT Features: Scar encodes Gly-Ser in-frame with prefix start codon; Employs...

... for the development of successful therapeutics. ATCC - (Link opens in a new window) LUHMES cell line ...largest biorepositories (Link opens in a new window) ATCC - the world's largest cell line repository Tissue...

...in the Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Article Tac1-Expressing Neurons in ...in the Periaqueductal Gray Facilitate the Itch-Scratching Cycle via Descending Regulation. Neuron 101(1...

...the selected target sequence. Additionally, any mismatches between a given gRNA sequence and your genomic...Method CRISPR Edit Type Additional Considerations Mismatch cleavage assay Knockouts Some point mutations ...

...with the complementary codon on the mRNA. If the match is correct, the amino acid carried by the tRNA is...a previously established protocol, make sure to match the growth medium, ncAA concentration, and the cell...

...delivery of a double-stranded RNA (dsRNA) that matches the mRNA target sequence. The dsRNA can be delivered...

...resulting heteroduplex is resolved by the cell’s mismatch repair (MMR) system. Alternatively, if the edited...

...Cre-dependent 8 Bryan Roth No available items found to match all requested criteria. Please consider using fewer...

...fluorophores - Andrew Fire Lab. Available with and without PATC, which diminishes epigenetic silencing in the germline...

...Transfer Plasmid Addgene uses a rigorous barcode matching system that follows the sample from the DNA tube...