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We narrowed to 80 results for: KAN

Showing: 1 - 20 of 80 results

  1. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ....   Kanamycin Kanamycin, or kan, is an aminoglycoside which inhibits protein synthesis. The kan resistance...resistance gene, NPTII, facilitates phosphorylation of kan, which inactivates it. The NPTII gene confers resistance...decorative" title="Check icon" %} Satellite colonies Kanamycin Excellent No Bacteria and eukaryotes (G418) {%...

  3. Bacterial Expression Systems

    Type
    Collection
    ...Methylobacterium extorquens Julia Vorholt 84693 pMyNT-kan Acetamidase promoter Acetamide Escherichia coli ,...Mycobacterium smegmatis Matthias Wilmanns 84692 pMyC-kan Acetamidase promoter Acetamide Escherichia coli ,...Mycobacterium smegmatis Matthias Wilmanns 84689 pMyBADC-kan pBAD Arabinose Escherichia coli , Mycobacterium smegmatis...) Escherichia coli Cynthia Collins 84689 pMyBADC-kan pBAD Arabinose Escherichia coli , Mycobacterium smegmatis...

  4. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...previously described pFA6a-link vectors and include a Kan, SpHIS5, or CaURA3 selection marker. Lee et al.(1...3xFlag, Protein A, or V5), and selection marker (KanMX, HphMX, or His3MX).  Each PCR product from this ...

  6. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...instead use G418. This can be confusing since the neo/kan gene confers G418 resistance; however, like gentamicin...

  8. Evolution of Lab Techniques

    Type
    Blog Post
    ...PubMed PMID: 1195397. 2. Chang, Judy C., and Yuet Wai Kan. "A sensitive new prenatal test for sickle-cell anemia...

  9. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Clover - Mammalian Expression pFA6a-link-yoClover-Kan - Yeast Expression dClover2 505 515 Dimer (A206K)...C1 - Mammalian Expression pFA6a-link-yoTagRFP657-Kan - Yeast Expression smURFP 642 670 32 3.2 39 min Prone...pRSETA-PAGFP - Bacterial Expression pFA6a-PA-GFP-kanMX6 - Yeast Expression pFA6a-link-yEPAGFP-CaUra3 - ...on our blog . Protein Ligand Plasmids HaloTag Chloralkane (CA) pMH-Halo tag - Mammalian Gateway Destination...

  10. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Basta Chen pKSE401 62202 Plant yes, cut S. pyogenes Kan Chen pHUE411 62203 Plant yes, cut S. pyogenes Hyg...Jackson pMZ376 74213 Yeast rrk1 yes, cut S. pyogenes KanMX Zaratiegui pMZ377 74214 Yeast rrk1 yes, cut S. pyogenes...

  11. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...frequencies in the central nervous system (CNS) (Wu, Asokan, et al., 2006). AAV1 has also been found to transduce...cells (Ahuja et al., 2025; Issa et al., 2023; Wu, Asokan, et al., 2006). AAV2-retro (also known as AAVrg...capsids from those of AAV1 (Issa et al., 2023; Wu, Asokan, et al., 2006). AAV6 is particularly well-suited...kidney, and retinal cells (Issa et al., 2023; Wu, Asokan, et al., 2006). AAV9 AAV9 has relatively broad ...varies by species and route of administration (Wu, Asokan, et al., 2006). Want to know if a capsid is a good... https://doi.org/10.1074/jbc.M101559200 Wu, Z., Asokan, A., & Samulski, R. J. (2006). Adeno-associated...

  12. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    ...with the cells’ endogenous ubiquitin ligase, the Kanemaki lab was able to translate AID to yeast, as well...Nishimura et al., 2009, Natsume et al., 2016).  The Kanemaki lab previously developed a CRISPR/Cas9 based method...essential proteins (Natsume et al., 2016). Now, the Kanemaki lab has created a new toolkit of plasmids for ...create conditional human cell lines. Lastly, the Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor...

  13. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...serotype can affect spread from the injection site  (Kanaan et al., 2017), axonal transport  (Murlidharan et...cells12050785 Murlidharan, G., Samulski, R. J., & Asokan, A. (2014). Biology of adeno-associated viral vectors...5), 1–41. https://doi.org/10.3390/cells12050785 Kanaan, N. M., Sellnow, R. C., Boye, S. L., Coberly, B...pharmaceutics13050750 Murlidharan, G., Samulski, R. J., & Asokan, A. (2014). Biology of adeno-associated viral vectors...

  14. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...signals when used with the same protein (Hayashi-Takanaka et al., 2014). Other dyes may prove challenging...and reliable imaging and assay results (Hayashi-Takanaka et al., 2014)​​. Conjugates with lower DOL tend...intracellular fluorescence live imaging ​​(Hayashi-Takanaka et al., 2014). Dyes can also vary in their brightness...994. https://doi.org/10.1038/nmeth.4403  Hayashi-Takanaka, Y., Stasevich, T. J., Kurumizaka, H., Nozaki,...

  16. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...activity after ischemic brain injury Dr. Gohar Tsakanova, Institute of Molecular Biology of the NationalAcademy...Sciences of the Republic of Armenia, Armenia Gohar Tsakanova is a Senior Researcher who investigates the molecular...two-photon laser scanning microscope will allow Tsakanova to image cortical neurons and study changes in...

  17. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Structure of a retrovirus. Image re-used from Dong & Kantor, 2021 under CC-BY license. Figure 2 gives a...1406488. PubMed Central PMCID: PMC372876 Dong, W., & Kantor, B. (2021). Lentiviral Vectors for Delivery of ...PubMed Central PMCID: PMC4515362 Robinson, W. S., Pitkanen, A., & Rubin, H. (1965). The nucleic acid of the...

  18. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... based on a bacterial haloalkane dehalogenase that binds a synthetic chloralkane (CA) ligand. The current...  (TMP-tag) Few Easy HaloTag 295 Chloralkane (CA) Many Easy CLIP-tag 182 Benzylcytosine...

  19. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...cleavage, KanR 26101 pET28GST-LIC EF456739 GST-tag and hexahistidine tag with Thrombin cleavage, KanR 26096...cleavage, KanR 26105 pNIC-CTHF EF199844 Hexahistidine tag, FLAG tag with TEV cleavage, KanR 26106 pNH-...MHL EF456735 Hexahistidine tag with TEV cleavage, KanR 26098 pCW-LIC EF460848 No tag, double ptac promoter...Hexahistidine tag, thioredoxin with TEV cleavage, KanR 26107 pNIC-ZB GU452710 Hexahistidine tag , Z-basic...

  20. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ... Petsko 74257 pKanCMV-mClover3-10aa-H2B Chromatin H2B mClover3 Michael Lin 74258 pKanCMV-mRuby3-10aa-H2B...74259 pKanCMV-mClover3-18aa-actin Actin filaments beta-Actin mClover3 Michael Lin 74255 pKanCMV-mRuby3...74253 pKanCMV-mClover3-18aa-Tubulin Microtubules alpha-Tubulin mClover3 Michael Lin 74256 pKanCMV-mRuby3... 74260 pKanCMV-CX4.3-7aa-mClover3 Gap junctions Connexin 43 mClover3 Michael Lin 74254 pKanCMV-CX4.3-7aa-mRuby3...
Showing: 1 - 20 of 80 results