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We narrowed to 205 results for: PREP

Showing: 1 - 20 of 205 results
  1. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...freshly collected lentiviral prep may be significantly higher than the same prep that has been stored in the...painstakingly cared for your packaging cell line, prepped your DNA, transfected and harvested your lentivirus...determine the amount of virus you actually have in your prep. Taking time to properly titer your virus will not...qPCR for viral RNA. Measuring p24 levels in a viral prep is straightforward and rapid as there are several...universal primers can be used to titer any lentiviral prep that shares that specific feature. Primers targeting...overestimate the amount of infectious virus in a prep as the measurement will include defective particles...following transduction with serial dilutions of a viral prep. This method is particularly useful when vectors...
  2. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    ...or a pre-made viral prep library. Addgene’s pooled libraries are typically DNA preps, but we do distribute...libraries as pre-made viral preps. If you can’t (or don’t want to) make viral preps in your lab, there are ...pooled library lentiviral prep. B) Usage of a pooled library lentiviral prep for experiments. Pooled library... use! In this post, I’ll walk you through how to prep and transduce your pooled library.  Pooled libraries...are custom viral prep services that can prepare the libraries for you.  Starting from plasmids requires ... library lentiviral preps are generally aliquoted to reduce the number of freeze-thaw cycles needed when...functional titer is determined, larger lentiviral preps can be used for screening-scale transduction of ...
  3. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: Expanded...INTRSECT viral preps from the Deisseroth Lab now available. Find all the Deisseroth viral preps here Coming...highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles.... Serotypes include AAV1, and AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato is now available... AAV5 and AAVrg. Find the Cre-expressing viral preps here AAV-CAG-dLight1.3b from the Lin Tian Lab ...Lab is now available as a ready to use viral prep in serotype AAV5! Get dLight1.3b here Looking for a...apoptosis. Now available as a ready to use viral prep in serotype AAV5. Find AAV-flex-taCasp3-TEVp here...
  4. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: Axon targeting...INTRSECT viral preps available! We are expanding our inventory of ready to use viral preps from the Deisseroth...highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles...pathways The CRISPR corner New ready-to-use viral preps Mapping antibody escape in SARS-CoV-2 by mutational...GCaMP8 vectors in serotypes AAV1 and AAV5. AAV9 preps and more AAV1 versions will be released soon. Visualize...project vectors available as ready-to-use viral preps with more on the way. S5E2 regulatory element containing...GCaMP6f are now available as a ready-to-use viral prep in serotype AAV1. Distinguish dendritic neurites...
  5. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...viral preps. Here are some of the new viral preps from recent months: New INTRSECT viral preps available...available as a ready-to-use viral prep in AAV1!  New ready-to-use AAV prep from Hongkui Zeng's lab are available...highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles...are expanding our inventory of ready to use viral preps from the Deisseroth lab INTRSECT project. Check ... lab, now available in a ready to use AAV5 viral prep.   Flp-dependent DREADDs from the Gether lab, now...
  6. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: Our selection...highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles...strigolactones The CRISPR Corner New ready-to-use viral preps New toolkit for CRISPR-based genome editing in...selection of GCaMP8 AAV preps has expanded. Check out the new selection of ready-to-use AAV9 and AAVrg vectors...
  7. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...to provide ready-to-use viral preps. Here are some of the new viral preps from recent months: Second generation...highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles... cells The CRISPR Corner New ready-to-use viral preps   Improved cell-free transcription/translation ... Dimidschstein Lab More GCaMP8 ready-to-use AAV preps  ...
  8. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...and comprehensive quality control. In-Stock Preps 1,000+ preps ready to ship immediately after MTA approval...protocols for how to use your viral prep and how to make your own preps. Recipient Instructions What to do...options so you can easily access the viral vector prep you need to get your experiments started more quickly...
  9. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...Instead of spending time and money producing virus from select vectors in the repository yourself, you...lentivirus (with many CRISPR tools included among the preps that are currently available) and adeno associated...faster by producing, titering, and testing viral preps for them. At the same time, we’re developing ourselves...scientists and accelerate research." What viral preps are available now? There are a wide variety of tools...already available from Addgene as ready-to-use viral preps and we’ll be continually expanding our collection...provide a brief rundown of what lentiviral and AAV preps are available below, but you can always head over...we’re starting the Viral Service with lentiviral preps for the delivery of Cas9 endonuclease, Cas9 nickase...
  10. AAV Viral Preps

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    Collection
    ... AAV preps are affordable, available in small volumes, and ready to ship. All viral vector preps are purified...and viral genome sequencing (VGS). In-stock AAV preps are distributed as 100 µL solutions of purified ...reported with each shipment. If you can't find the prep you need, try Addgene's AAV Packaged on Request ... pages above. You can also do a search of viral preps and filter by the serotype needed....
  11. Recombinases AAV Preps

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    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of...AAV Browse the following tables for in-stock AAV preps that encode site-specific recombinases, which can...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...
  12. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...viral prep with multiple serotypes pAAV-hSyn-GRAB_OTmut AAV1 Biosensor Yulong Li New viral prep pAAV-CAG-NIR-GECO2G...Fyhn New viral prep AAV-hSyn-Ribo-jGCaMP8m AAV1 Biosensor Marianne Fyhn New viral prep pAAV-Ef1a-fDIO... viral prep pAAV_BiSSTe10_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New viral prep pAAV_... viral prep pAAV_BiCHATe27_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New viral prep pAAV_BiCHATe27...New viral prep pAAV_BiVIPe4_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New viral prep pAAV_... viral prep pAAV_BiLAMP5e3_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral prep pAAV_...New viral prep pAAV_BiPVe4_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral prep pAAV_BiPVe3...
  13. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ... new preps available in the Addgene viral vector repository! This includes the first Addgene prep packaged...viral prep pAAV_hSyn-DIO-PdCO-mScarlet-WPRE AAV1, AAV5 Optogenetics Ofer Yizhar New viral prep with...Lin Tian New viral prep pAAV-hSyn-GRAB-gDA3m AAV1 Biosensors Yulong Li New viral prep pGP-AAV-GFAP-iGABASnFR2...part of the BRAIN Armamentarium Collection. These preps drive expression of either Cre recombinase or a ...Recombinases Stanley Thomas Carmichael New viral prep pAAV-EF1a-fDIO-Cre AAV5 Recombinases Esteban ... AAV PHP.eB Recombinases Jonathan Ting New viral prep AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T... AAV PHP.eB Recombinases Jonathan Ting New viral prep AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA...
  14. Control AAV Preps

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    Collection
    ...We provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...
  15. Chemogenetics AAV Preps

    Type
    Collection
    ...eligible AAV plasmids and we’ll make the viral vector prep you need. Or fill out our form to let us know what...what you would like to see available in viral prep format. Please note this does not guarantee viral vector...
Showing: 1 - 20 of 205 results