We narrowed to 399 results for: SEF
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TypeCollection...which highlights plasmids available that may be useful for COVID-19 related research. Additionally, we...Cas13a, Reverse Transcriptases, etc. that may be useful for detection assays and more. Pooled Libraries...-19 research. Protocols : Protocols that may be useful for COVID-19 research. Antibodies Anti-SARS-CoV...you have a suggestion for plasmids that would be useful for your COVID-19 research? Fill out this Suggest...
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Zebrafish Plasmid Collection
TypeCollection...Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR resources can be found below: ...and track clones of cells over time. Examples of useful tools for studying cells that share clonal origin...Protein Fusion Construct Set - Amro Hamdoun Lab. A useful “starter kit” for screening fluorescent protein... -
Neurodegeneration Research Collection
TypeCollection...plasmid collections available at Addgene that may be useful for neurodegeneration research....Noteworthy: AAV.rTH.PI.Cre.SV40 ready-to-use viral prep useful for dopamine research. CBA-driven, Cre-dependent...related disorders. The site curates information on useful resources such as: Antibodies (Link opens in a ... -
Cre-Lox and Other Site-Specific Recombinases
TypeCollection...Cre , Flp , Dre , other recombinases , as well as useful empty backbones or reporters . Experimental Considerations...event. Toxicity Cre-lox recombination is a very useful tool, but Cre expression can be toxic in some systems...undergone recombination. Reporter plasmids can also be useful for lineage tracing, evaluating infection/transfection... -
Luciferase Plasmid Collection
TypeCollection...luciferase have short protein half-lives, making them useful as transcriptional reporters, but are less-than-ideal...an illumination source. BRET can be particularly useful for in vivo imaging or creating biosensors. Read... And see PalmGRET , a membrane-anchored version useful for labeling extracellular vesicles. LumiScarlet... -
Plan Your Experiment
TypeCollection...transfect. However, note that this method is most useful for cell lines that you can transfect at high efficiency...types by using cell-specific promoters, which is useful for in vivo studies. While we don’t discuss CRISPR...population. Additionally, clonal populations are useful for determining if your edit is homozygous or heterozygous... -
Protocol - How to Perform a Diagnostic Digest
TypeProtocol...have a map and know exactly what it should be. A useful restriction enzyme based technique for verifying... -
Centrifugation
TypeProtocol...minipreps and RNA extractions. Plus, centrifuges are useful for simply collecting liquid to the bottom of a... -
Antibody Plasmid Collection
TypeCollection... to being useful for our bodies to fight off intruders, antibodies are also extremely useful to researchers... -
Worm Expression Resources
TypeCollection... resources for the community by depositing your useful tools , or by suggesting plasmids for the collection...constructs for endogenous tagging. Some examples of useful worm CRISPR resources, along with links to protocols... -
Promega Plasmid Collection
TypeCollection...tags such as NanoLuc, HaloTag, SmBiT, and LgBiT, useful for detecting and tracking key drug target proteins...endogenous expression levels. NanoLuc fusions are useful for both intracellular and extracellular applications... -
Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps
TypeProtocol...Page . We hope that you'll find these protocols useful in your own work. Please feel free to email us ... -
Gibson Assembly Protocol
TypeProtocol...together several oligos. This technique is especially useful for introducing promoters, terminators, and other... -
Plant Plasmids and Resources
TypeCollection...to individual plasmids, Addgene carries several useful toolkits and plasmid collections for use in plants...Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR resources include: A Multipurpose ... -
General Transfection
TypeProtocol...gene of interest in a target cell line and can be useful for short term studies of protein function. We ... -
Protocol - Bacterial Transformation
TypeProtocol...cells. This is a relatively simple procedure and is useful for performing low efficiency transformations. ... -
Protocol - How to Ligate Plasmid DNA
TypeProtocol...Background due to vector re-circularization - most useful for phosphatase treated vector Insert or water ... -
Rinehart Lab Phosphoprotein Reagents
TypeCollection...The Rinehart Lab has developed several tools useful for the incorporation of phosphoserine into your ...library (Pooled Library #111704) is anticipated to be useful for laboratories performing phosphoproteomic experiments... -
Rett Syndrome
TypeCollection...previously acquired skills, notably loss of acquired purposeful hand movements and regression of speech. In contrast...syndrome of autism, dementia, ataxia, and loss of purposeful hand use in girls: Rett’s syndrome: report of... -
Plasmid Cloning by Restriction Enzyme Digest (with Protocols)
TypeProtocol...correct orientation in the recipient plasmid, it is useful to see if one set of enzymes will work in the same...