We narrowed to 105 results for: Ubc

...and selected subcellular marker proteins, it’s possible to get an idea about the subcellular structures ...protein function. Getting ready to determine the subcellular localization of your protein of interest The ... vesicles via the secretory pathway to their subcellular or extracellular destination. At the end of their...proteins tagged with FPs to highlight various subcellular structures. Please see Figure 1 for commonly ...inert fluorescent protein fusions to visualize subcellular structures. For example, you might use your fluoresent...particular gene. For subcellular markers in yeast see our plasmid collection from...You can find more plasmids for labeling your subcellular structure of interest in the Allen Institute ...

... Fluorescent Proteins Subcellular Localization Fluorescent Proteins: Subcellular Localization General ...encoding fluorescent fusion proteins with known subcellular localization patterns for colocalization studies...Membrane Trafficking Additional Resources The subcellular localization of a protein is often closely related...There are a variety of methods for determining subcellular localization of a protein of interest. One of...proteins that are targeted to various organelles or subcellular structures. To compare with a well-characterized...Eukaryotic cells contain a variety of organelles and subcellular structures, each with their own unique function...

...Renilla Ubc Lentiviral Expression of Renilla luciferase Rudolf Jaenisch 113450 pLenti NL NanoLuc® Ubc Lentiviral... luciferase Christopher Vakoc 33307 Ubc.Luc.IRES.Puro Firefly Ubc Lentiviral expression of firefly luciferase...luciferase Reuben Shaw 48688 pUltra-Chili-Luc Firefly Ubc Lentiviral expression of firefly luciferase and dTomato...

... constructs: pLVX-UbC-rtTA-Ngn2:2A:EGFP (Schafer et al. Nat Neurosci. 2019) pLVX-UbC-rtTA-Ngn2:2A:Ascl1... Use the 3xFlag-eGFP-Flag-SETX construct for subcellular localization studies with GFP, or IP with Flag...of fluorescent proteins, including Markers of Subcellular Localization , Optogenetics Plasmids , and Chemogenetics...

...Ultra-Exon1Q23-Myc-A HTT Myc UbC Huntington's Baoji Xu 110487 Ultra-Exon1Q145-Myc-A HTT Myc UbC Huntington's Baoji...pUltra-Exon1Q23-Myc-B HTT Myc UbC Huntington's Baoji Xu 110489 pUltra-Exon1Q145-Myc-B HTT Myc UbC Huntington's Baoji...FUGW-APPwt-T2A-mCherry APP mCherry UbC Alzheimer's Ronald Hart 190804 FUGW-APPSwe-T2A-mCherry APP mCherry UbC Alzheimer's Ronald...FUGW-APPSwe-T2A-Hygro APP UbC Alzheimer's Ronald Hart 190806 FUGW-APPIce-T2A-mCherry APP mCherry UbC Alzheimer's Ronald...APOE mCherry UbC Alzheimer's Ronald Hart 190808 FUGW-APOE-E3-T2A-mCherry APOE mCherry UbC Alzheimer's ...T2A-mCherry APOE mCherry UbC Alzheimer's Ronald Hart 190810 FUGW-APOE-E4-T2A-Puro APOE UbC Alzheimer's Ronald...pTet-O-APOE-E2-T2A-Puro APOE UbC Alzheimer's Ronald Hart 190812 pTet-O-APOE-E3-T2A-Puro APOE UbC Alzheimer's Ronald...

...regulation due to methylation or deacetylation. Ubc General expression mRNA Mammalian promoter from the...

...promotes cancer. She is also a science communicator, UBC Public Scholar, and a member of the Vancouver SciCATs...

... Empty Backbones Mammalian CMV, SV40, EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian...

...FUW-M2rtTA Lentiviral Tet-On vector, expressing rtTA from UbC promoter rtTA-Advanced Rudolf Jaenisch 25434 pMA2640...

...Human U6 promoter Forward hUBCpro-F TGAAGCTCCGGTTTTGAACT Human Ubiquitin C (UbC) promoter Forward IRES-F...

...Human U6 promoter Forward hUBCpro-F TGAAGCTCCGGTTTTGAACT Human Ubiquitin C (UbC) promoter Forward IRES-F...

...enzyme digest (subcloning), including design and experimental procedures.... Protocols Subcloning Plasmid Cloning...Cloning by Restriction Enzyme Digest (aka Subcloning) You may also like... Restriction Digest of Plasmid...out our Empty Backbone Reference . Background Subcloning by restriction digest is a commonly used lab ...YGOI is in a bacterial expression vector. Using subcloning, you can easily move YGOI into a mammalian expression...find two different restriction enzymes for your subcloning. It is also possible to use a single enzyme, ...

...enzyme digest (subcloning), including design and experimental procedures.... Protocols Subcloning Plasmid Cloning...Cloning by Restriction Enzyme Digest (aka Subcloning) You may also like... Restriction Digest of Plasmid...out our Empty Backbone Reference . Background Subcloning by restriction digest is a commonly used lab ...YGOI is in a bacterial expression vector. Using subcloning, you can easily move YGOI into a mammalian expression...find two different restriction enzymes for your subcloning. It is also possible to use a single enzyme, ...

...function. We specifically use this protocol with a subclone of HEK293T cells optimized for viral production...Workflow Timeline Day 0: Seed HEK293T cells (or a subclone of HEK293T optimized for viral production) Day...

...function. We specifically use this protocol with a subclone of HEK293T cells optimized for viral production...Workflow Timeline Day 0: Seed HEK293T cells (or a subclone of HEK293T optimized for viral production) Day...

...different subclasses, but there are also secondary antibodies that are specific to each subclass. Approximately...mouse mAbs are IgG1 subclass (Manning et al. 2012), which limits the use of subclass specific secondary...backbone to generate R-mAbs of different subclasses, including subclasses that were not produced by the original...original hybridoma. By using subclass specific secondary antibodies and subclassed switched R-mAbs, multiple...engineered. Like humans, mice express multiple subclasses of IgG antibody. Broad range secondary antibodies...  Fig. 2. Multiplex immunolabeling with subclass-switched recombinant antibodies in adult rat brain...KD, Engebrecht J, Trimmer JS. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

...different species, or at least from different IgG subclasses, so that secondary antibodies exclusively recognize...Must use different species and/or different IgG subclasses of 1° for multi-color staining Can be expensive...limited cross-reactivity between different IgG subclasses and different species, nanobodies are useful ...require using primary antibodies from different IgG subclasses. Instead, you can pre-incubate individual primary...antibody: species it was raised in and its IgG subclass. Once you know the answer to these two questions...right nanobody for your work. Species Antibody Subclass Nanobody Addgene Plasmid Rabbit N/A* TP897... (3x cysteines) *Rabbits have only one IgG subclass.   Express the Nanobody In Bacteria: Table 2 also...

...and α. Some isotypes have different versions or subclasses that have minor differences in their heavy chain...the hinge region in IgM antibodies. IgM has no subclasses and is found mostly in blood. IgD While IgD is...monomer structure with a valency of two and has no subclasses. IgG IgG is the most abundant isotype in blood...monomer with a valency of two. There are four IgG subclasses, numbered based on their abundance in blood: ...involved in allergic reactions. There are no IgE subclasses and they are expressed as monomers with a valency...dimer with a valency of four. There are two IgA subclasses: IgA1 and IgA2.  You can learn more about isotype...

...antibodies contain a mix of different isotypes and subclasses. They each have their own advantages. Since monoclonal...species, like an anti-mouse IgG antibody. Some are subclass specific, like an anti-mouse IgG2a antibody. When...    Figure 1: Broadly reactive versus subclass-specific antibodies. Only a fraction of the antibody... antibody (A). To boost signal, consider using subclass specific secondary antibodies instead (B). ...antibody contains a mix of different isotypes and subclasses, use a broadly reactive secondary antibody to...antibody you may wonder, “Will the isotype, and subclass affect my experiment?” Well, it depends on the...

...0.02–0.05 ml Skill required: beginner Subcutaneous Subcutaneous (SQ or SC) injections are given between...method, subcutaneous injections take some practice to get the coordination down. Subcutaneous injections...restrainer Thigh 25–27 < 0.02–0.05 mL Beginner Subcutaneous Back scruffing or mechanical restrainer Back...