We narrowed to 220 results for: rato cre

... plasmid toolkit, the Broad Institute Target Accelerator Plasmid Collection, that we hope researchers ... For many years, we’ve hoped that large scale laboratory-based functional experiments that classify which...and cancer phenotypes. Broad Institute Target Accelerator Plasmid Collections To overcome this first challenge..., we’ve developed the Broad Institute Target Accelerator Plasmid Collections, now widely available to ...Collection). In addition, we worked with many collaborators to aggregate a sequence-confirmed series of ...many major cancer genes. These should be used to create ever more complete “look-up-tables” of mutant function...

...School and Paratore is the General Auditor at Tufts Health Plan. The addition of Katz and Paratore onto the...the potential for increased speed and efficiency through which knowledge is created and shared. Can you...Interactive. Learn more about Carl Paratore, MBA, CPA Mr. Paratore serves as the General Auditor at Tufts...re excited to welcome Karen Katz, Esq and Carl Paratore, MBA, CPA to the Addgene Board of Directors. Katz...that Addgene was looking for new board members. Paratore: Kristen Darby, who’s also a board member, reached...necessary information that’s required to do research. Paratore: What I’ve been most impressed with is the level...platform. Expanding further beyond the US and increasing Addgene’s international presence would potentially...

...AAV-pgk-Cre PGK Cre expression Recombinases Patrick Aebischer 55632 pAAV-Ef1a-mCherry-IRES-Cre EF1a Cre...pENN.AAV.CamKII 0.4.Cre.SV40 CamKII Cre expression Recombinases James M. Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato... by using this serotype to deliver Cre-dependent transgenes in Cre transgenic lines. As part of our standard...pAAV-hSyn-DIO-EGFP Syn EGFP, Cre-dependent Control Bryan Roth 55650 pAAV-hSyn Con/Fon EYFP Syn EYFP, Cre and Flp-dependent...CAG tdTomato, Cre-dependent Control Edward Boyden 27056 pAAV-Ef1a-DIO EYFP EF1a EYFP, Cre-dependent Control...Expresses NLS-mCherry in the absence of Cre, and expresses NLS-EGFP in Cre-positive cells. Control Brandon Harvey...Recombinases Karl Deisseroth 55636 pAAV-EF1a-Cre EF1a Cre expression Recombinases Karl Deisseroth 55637...

...Cre-expression Cre Wilson 107738 pAAV-hSyn-Cre-P2A-dTomato Syn Cre-expression Cre Larsen 140135 pAAV-EF1a-iCreV...pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn Cre-EGFP expression Cre Wilson 105550 pAAV.GFAP.Cre.WPRE.hGH GFAP Cre-expression...Activator, Cre-dependent DREADD Roth 44362 pAAV-hSyn-DIO-hM4D(Gi)-mCherry Syn Inhibitor, Cre-dependent ...sensor, Cre-dependent GCaMP Kim , GENIE 104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE Syn Calcium sensor, Cre-dependent...sensor, Cre-dependent GCaMP Kim , GENIE 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG Calcium sensor, Cre-dependent...Activator, Cre-dependent ChR2 Deisseroth 127090 pAAV-CAG-DIO-ChR2(H134R)-eYFP CAG Activator, Cre-dependent... Controls 28306 pAAV-FLEX-tdTomato CAG tdTomato, Cre-dependent Control Boyden 37825 pAAV-CAG-GFP CAG GFP...

...expression of GCaMP6m/s in cells depdent on Cre, Flp, or Vcre Comprehensive Dual- and Triple-Feature Intersectional...fluorescent protein-based calcium integrator (CaMPARI) (Constitutive or Cre-dependent) Neural circuits. Labeling...Genetically Encoded Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically Encoded Calcium...High-performance GFP-based calcium indicators (Constitutive or Cre-dependent) High-performance calcium sensors for imaging...sensors for imaging neural activity (Constitutive or Cre-dependent) Ultrasensitive fluorescent proteins for...calcium sensor jYCaMP1 variants (Constitutive or Cre-dependent) jYCaMP: an optimized calcium indicator...jRCaMP1b) and mApple (jRGECO1a) (Constitutive or Cre-dependent) Sensitive red protein calcium indicators...

...in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors...AiP13781 - pAAV-AiE0452h-minBG-iCre(R297T)-BGHpA (Alias: CN3781) Enhancer AAV for Cre recombinase expression ...pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779) Enhancer AAV for Cre recombinase expression ...AiP13778 - pAAV-AiE0450h-minBG-iCre(R297T)-BGHpA (Alias: CN3778) Enhancer AAV for Cre recombinase expression ...PHPeB CN1851-rAAV-hI56i-minBglobin-iCre-4X2C-WPRE3-BGHpA AAV vector for Cre expression in forebrain GABAergic...pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) Expression of iCre(R297T) in striatal medium...will aid neuroscientists in studying circuits in laboratory animals and human tissue specimens. These technologies...

...Neo-TRE-CMV-Cre-rtTA Dox-inducible expression of Cre recombinase. Madeline Lancaster 198752 DiLiCre 2.0 Dox-inducible...85040 pK170.AAV-TRE-Cre-WPRE (Supernova) AAV vector for dox-inducible expression of Cre recombinase. Takuji...63704 pRetroX GFP T2A Cre Retroviral vector for dox-inducible expression of GFP T2A Cre recombinase. Floris...Dox-inducible expression of a light-activated Cre recombinase. Jacco van Rheenen 104102–104112 TetBow Series...: Repressible Promoters Mammalian shRNA Plasmids Cre-lox Plasmids Stem Cell Research Plasmids References... the tetracycline repressor protein (TetR) and operator ( tet O, a 19 nucleotide sequence, TCCCTATCAGTGATAGAGA...tetracycline-controlled transactivator (tTA) was created by fusing TetR with the activation domain of the...

...resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically controlled Cre/loxP system that ...toolbox of genetic methods, such as Tol2 transposons, Cre-mediated site-specific recombination, morpholinos...fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large collections or kits are...that are amenable to genetics, high-throughput screening and non-invasive live imaging. A standard toolbox... Amro Hamdoun Lab. A useful “starter kit” for screening fluorescent protein fusion expression in organisms...antibodies to zebrafish cell surface receptors and secreted proteins. Return to top Other Resources Addgene...American Zebrafish Network (LAZEN) - LAZEN aims to increase the use of zebrafish as a model organism and to...

...RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids. Large collections or kits are... approach has also been developed by the Fire laboratory and described in Efficient Marker-Free Recovery...

...pCFJ90 (pharyngeal co-injection marker); pDD104 (Cre recombinase) PDF, 105 KB Jaenisch CRISPR plasmid ...- a CRISPR Screen Tool (Link opens in a new window) Design gRNAs for custom CRISPR screens targeting a...in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's downloadable eBook about...CRISPR pooled libraries for large-scale functional screens. Available for knockout, activation, inhibition...For use with genome-scale CRISPR-Cas9 knockout screens (or GeCKO) technology. Developed by the Xiaole ...batch (Link opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new window) ... Lab and maintained by the Frederick National Laboratory at the National Cancer Institute. Cas-OFFinder...

... and the screen results. Screenshots updated 2024. Importantly, metadata for each screen is captured...CRISPR screen data that might be able to help you out. What is the Open Repository of CRISPR Screens? The...BioGRID) in order to increase the accessibility of CRISPR-based phenotypic screen datasets. Original published...BioGRID Open Repository of CRISPR Screens (ORCS) homepage. Screenshot updated 2024.   Using the Open...Repository of CRISPR Screens BioGRID ORCS currently houses over 890 CRISPR screens in 629 cell lines, each... browse through screens performed in relevant cell lines or conditions. This increased ease of access ...cancer" returns 40 screens performed in 26 different breast cancer cell lines. Screenshot updated 2024. ...

...they were created. Find their contact information by following the link to their laboratory website. ... working with the RSRT along with individual laboratories to assemble a Rett Syndrome plasmid resource...

...Plasmid Tools Pooled Libraries Other Resources Image credit: Maya Kostman for the Innovative Genomics Institute...), including plasmids for protein production, secretion, and codon-optimized versions designed...metalloprotease that cleaves hACE2, potentially increasing viral infectivity (Heurich et al., 2014). PCP4...-CoV-2 Spike (S) Ectodomain and RBD Libraries Screening Timothy Whitehead Libraries of Spike (S) Ectodomain...Binding Domain Deep Mutation Scanning Library Screening Jesse Bloom Library of Spike protein RBD mutations... Domain Site-Saturation Mutagenesis Libraries Screening Jesse Bloom Tyler Starr Libraries of variant Spike...Cathepsin L functionally cleaves the severe acute respiratory syndrome coronavirus class I fusion protein upstream...

...Upon activation, NK cells secrete perforin and granzyme B, two enzymes that create pores within an infected...LSIGN, MGC129964, MGC47866 CREB1 cAMP responsive element binding protein 1 CREB, MGC9284 CTSB cathepsin ...chromogranin A (parathyroid secretory protein 1) CGA CHGB chromogranin B (secretogranin 1) SCG1 CKLF chemokine-like... SDS, SWDS SCG2 secretogranin II (chromogranin C) CHGC, SN, SgII SCGB3A1 secretoglobin, family 3A, member...HIN1, LU105, MGC87867, PnSP-2, UGRP2 SCT secretin - SCTR secretin receptor SR SCYE1 small inducible cytokine... TDGF1 teratocarcinoma-derived growth factor 1 CR, CRGF, CRIPTO, Cripto-1 TDGF3 teratocarcinoma-derived...

...and Libraries for Cancer Research Broad Target Accelerator Plasmids: Collection of vectors from the Boehm...mutant alleles described in the initial Target Accelerator publications, focused on somatic variants found... Tackling Cancers’ Drug Resistance with a New Screening Kit (David Sabatini & Kris Wood) National Cancer...

...Once the recipient cells take up the Cre-tagged mitochondria, Cre is released, translocates to the nuclease...mitochondria (Hoover et al., 2025). MitoTRACER relies on a Cre-lox switch to turn recipient cells from red to green...green fluorescence (Figure 2). Donor cells express iCre with a mitochondrial anchor and a TEV cleavage site...nuclear localization signal; TEVp = TEV protease. Created with BioRender.com. Using this system, the...and melanoma models, showing that this transfer increases the metabolic and metastatic capacity of cancer...macromolecules to serve cellular functions. Along with collaborators, the Denes Hnisz Lab previously discovered that...models By Brian O'Neill The Nadav Ahituv Lab and collaborators have found a way to make adipocytes fight cancer...

... vivo screens in naïve T cells. More broadly, this system can be used to perform in vivo screens in other...power of in vivo screens. Figure 1: Using FITS to perform in vivo CRISPR screens to assess T cell...FITS In vitro CRISPR screens in immune cells have identified many putative regulators of immunity. However...However, in vitro screens cannot faithfully model the complex spatial, cellular, metabolic, and cytokine...vivo. Over the past five years, in vivo CRISPR screens have been used to interrogate T cells, an important...complex and physiological microenvironments. These screens have primarily been performed in activated T cells...naïve T cells. The Framework for In vivo T cell Screens (FITS) approach (Milling and Markson et al., 2024...

...technical skills, and experiences from thousands of laboratories that makes up our Help Center, blog, website...provide informative content to help researchers in creating and sharing knowledge, related to the types of...Identify needed content Let’s take a look at how we create this content, using the example of our Antibody...Tweet, followed by very detailed answers from our incredible scientists. Expert reviews on blog posts are ...need to reach out to an external expert to help us create accurate content. Regardless of the process, everything...

...find their publications in the first place. As a curator of the thousands of amazing plasmid technologies...they need to use them in their own work, it also increases the visibility of both you and the original depositor...work later. We also hope that this feature will decrease redundancy. If you modify a plasmid from Addgene...out there Beyond these 3 big pushes, we try to increase your work’s visibility in a few other ways. Every...

... floxed dCas9 so that it may later be excised by Cre recombinase. Addgene has several great options: pLV...T2A-GFP for removable dCas9 expression and pLM-CMV-R-Cre. Selection for cells stably and highly expressing...Daniel M. Sapozhnikov, a graduate student in the laboratory of Dr. Moshe Szyf at McGill University. Some ...are dioxygenases that oxidize 5-methylcytosine to create rare DNA bases that are recognized by distinct ...Daniel M. Sapozhnikov is a graduate student in the laboratory of Dr. Moshe Szyf at McGill University, working...