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...the S. cerevisiae lineage (7). In our class research project, students transform S. cerevisiae met deletion...synthesis (5). The budding yeast, Saccharomyces. cerevisiae, makes a great reference organism for the project... is available (6). Methionine synthesis in S. cerevisiae (Figure 1) occurs through a well-characterized...the same function as their counterparts in S. cerevisiae. The two yeasts are thought to have diverged ...carrying MET/Met genes from either S. pombe or S. cerevisiae. Students then determine if expression of the...plasmids in either E. coli (ori and ampR) or S. cerevisiae (2µm ori and URA3). The presence of the URA3 ...transformed with the plasmids, because the S. cerevisiae deletion strains are ∆ura3 mutants and cannot...

...and it grows fast to boot. The yeast strains S. cerevisiae and S. pombe have dominated the research scene...used yeast strains: S. pombe (fission) and S. cerevisiae (budding). Yeast make for an excellent model ...genetic manipulation The first eukaryote (S. cerevisiae) to have its genome fully sequenced There are...wondering if the “biotech yeast” is S. pombe or S. cerevisiae. Actually, it’s neither. The most commonly used...phaffii and other yeast strains.     S. cerevisiae S. pombe K. phaffii Growth properties... is only distantly related to S. pombe and S. cerevisiae; evolutionarily it evolved much slower and bears...evolutionary feature of K. phaffii, relative to S. cerevisiae, is that it predominantly uses non-homologous...

...L-hisitidine no S. cerevisiae  no yes   URA3 pyrimidine (uracil) yes - Grow with 5-FOA. S. cerevisiae yes - This...of a nitrogen source. S. cerevisiae  no no   LEU2 L-leucine no S. cerevisiae yes - This can complement...pertains to ORIs in budding yeast, Saccharomyces cerevisiae; however, we’ve also noted some features required... TRP1 L-tryptophan yes - Grow with 5-FAA. S. cerevisiae no  no TRP1 alters some yeast phenotypes. This...hydrosulfide ions yes - Grow with methylmercury. S. cerevisiae  no no Can be used for color and growth selection...

...integrate protein tags into the genomes of S. cerevisiae and S. pombe. The protocol is surprisingly simple...assessed many of these fluorescent tags in S. cerevisiae, looking at their performance in categories such...a complimentary set of plasmids for use in S. cerevisiae, with the additional benefit of multiple selection...

...metabolites in Escherichia coli or Saccharomyces cerevisiae with few techniques that are easily transferrable...oft published metabolic engineering realm of S. cerevisiae and E. coli. The utility of simultaneous and... of this to control a metabolic network in S. cerevisiae by using three RNA programs to differentially...

...the MoClo YTK Kit specifically designed for S. cerevisiae is at your service (Lee et al., 2015). This kit...2017). In addition to additional elements for S. cerevisiae chromosomal integration and gene expression, ...Extensive and Flexible Engineering of Saccharomyces Cerevisiae.” ACS Synthetic Biology, vol. 12, no. 11, American...

...-CoV-2 protein coding genes in E. coli and S. cerevisiae expression vectors are available at Addgene. ...-CoV-2 protein coding genes in E. coli and S. cerevisiae expression vectors in late March. The first set...

... yeast can be used for protein expression, S. cerevisiae, is the most reliable and frequently used species... in genetics and biochemistry. When using S. cerevisiae, researchers often place recombinant proteins...

...hybrid systems were developed in Saccharomyces cerevisiae in 1989 and are still used extensively to screen...heterologous systems (e.g. mammalian proteins in S. cerevisiae). While this may be difficult to resolve for...

...invert or ‘flip DNA’, is derived from the yeast S. cerevisiae (Gronostajski and Sadowski 1985). The FLP system...1985. “The FLP Recombinase of the Saccharomyces Cerevisiae 2 Micron Plasmid Attaches Covalently to DNA via...

...(Pfeiffer et al., 2010). Yeast (Saccharomyces cerevisiae) Yeast, one of the simplest eukaryotic organism...190-195. Liu, Wei, et al. "From Saccharomyces cerevisiae to human: The important gene co‑expression modules...

...repression of gene expression in Saccharomyces cerevisiae. The system consists of dCas9, the mammalian ...

...toxicity. FLP/FRT recombinases (from Saccharomyces cerevisiae) had been used successfully in Drosophila, so...

...that is supposed to be extremely bright. In S. cerevisiae, we've tested a number of green and red fluorescent...

...RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method. Mol Cell...

...recombination event in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another site-...

...editing with a single Cas9-VPR in Saccharomyces cerevisiae. To control Cas9 activity and influence DNA repair...

...melanogaster (fly), C. elegans (nematode), S. cerevisiae (budding yeast), S. pombe (fission yeast), A....

...inducible CRISPRi library - Ampl133 targets S. cerevisiae genes with 6-12 guides per gene where gRNA expression...

... vectors - PCR-based C-terminal tagging in S. cerevisiae pCS2FLAG - N- or C-terminal 2xFLAG...of proteins on the extracellular surface of S. cerevisiae cells His-tagged versions of pFastBac LIC vectors...Plasmids page pBF3060 - Metabolic engineering in S. cerevisiae TALENs Gene targeting TALEN kits - Construct ... and expression of bacterial gene clusters S. cerevisiae plasmids for multiple marker selection TRP1 Yeast...