We narrowed to 64 results for: stac

...expressed and purified as described previously in Stachelhaus et al. (1998) References George N, Pick H, Vogel...J Am Chem Soc. 126(29):8896-7. PMID:15264811 Stachelhaus T, Mootz HD, Bergendahl V, Marahiel MA.1998. ...

...transfection in: 15 cm dishes OR CF5 stacks Select how many dishes or stacks you plan to transfect: 1 to ...following μg of each plasmid needed per dish or stack. Calculations are based on using 28 μg of total ...) per 15 cm dish or 2500 μg of plasmids per CF5 stack. Total ug of each plasmid needed for the whole experiment...

...transfer stack. Set the Top Stack to one side and discard the white separator. Keep the Bottom Stack in the...Prestained protein ladder Ethanol iBlot 2 PVDF Mini Stack, Thermo Fisher IB24002 20X TBS Tween-20 Nonfat milk...the plastic tray. Place the Bottom Stack on the blotting surface. Align the electrical contacts on the...place it on the transfer membrane of the bottom stack. Soak a piece of iBlot Filter Paper in deionized... the air bubbles with the roller. Place the Top Stack over the pre-soaked filter paper. Remove air bubbles...roller. Place the Absorbent Pad on top of the Top Stack such that the electrical contacts are aligned with...

...improve resolution, use a stacking gel on top of the resolving gel. A stacking gel typically has a different...of the proteins in a sample migrate through the stacking gel at the same pace and enter the resolving gel...Figure 2: The western blot dry apparatus includes a stack of filter paper, a copper anode/iblot gel matrix...

... Many thanks to our guest blogger Sean Stacey! Sean D. Stacey is a Ph.D. student at East Tennessee State...This post was contributed by guest blogger Sean Stacey who recently attended both online and on site courses...

...of the manager is not to empower but to remove obstacles.” – Scott Adams, Dilbert cartoonist What makes...for example. So aside from a focus on removing obstacles, what makes a good manager? First, it takes an...their present positions By removing the primary obstacle of job dissatisfaction, the work can get done,...

...named NeuroStack was used (Orguc et al., 2020). Using a programmable microcontroller, NeuroStack coordinates...tissue. They performed optical stimulation using NeuroStack and the fibers as previously described, then ...

... Many thanks to our guest blogger Sean Stacey! Sean D. Stacey is a Ph.D. student at East Tennessee State...This post was contributed by guest blogger Sean D. Stacey. Opinions on the apps discussed in the article ...

...conversation about GMOs.  Challenge #2: Technical obstacles to plant synthetic biology In addition to public...understanding of plant biology is a significant technical obstacle to plant synthetic biology. We cannot build what...color-changing flower, and we’d love to hear about the obstacles you’ve faced as a plant scientist, and how you...

...membranes together and can wrap the membranes into stacked structures including organized smooth endoplasmic...of tubules can be grossly distorted into dense stacked membranes termed Organized Smooth Endoplasmic Reticulum...References 1. Snapp, E.L., et al., Formation of stacked ER cisternae by low affinity protein interactions...

...flask, Corning 430825, 175 cm 2 Cellstack 5, Corning 3319, 3180 cm 2 Cellstack 2, Corning 3269, 1272 cm 2 ...used to produce AAV from one Five Chambers Cell-Stack (CS5) (Link opens in a new window) (3,180 cm 2 -...the same surface area as 21 x T-175 flasks). Cell stacks provide an efficient means to scale-up without ...

...nothing if not solving your way through a series of obstacles in pursuit of achieving knowledge. Or, as we in...result of competing desires or (3) the presence of obstacles that need to be overcome. Conflict, and its inherent...

...another optimized platform. Gene Stacking As the name implies, gene stacking is the use of a combination of...

...diseases, blindness, and diabetes. Directions and obstacles for future CRISPR therapies CRISPR technology ...systemic approaches, has its own unique set of obstacles. Thus far, several targeted therapies have taken...

...genome editing applications. So, how does NgAgo stack up thus far? A few weeks ago, I was going through...Specificity with eSpCas9 and SpCas9-HF1 See How Cpf1 Stacks Up to Cas9 and NgAgo Resources on Addgene.org ...

...reducing the time it takes to image through a large z-stack (8).  One limitation of LSFMs using single side ...illumination, especially with large tissue, is that any obstacles (e.g. air bubbles or high concentration of fluorophores...

...how CRISPR can take on HIV-1 in vitro, and what obstacles must be overcome for clinical success. ART and...ART-induced viral control found in most HIV-1 patients. Obstacles for CRISPR HIV-1 therapy Both of these methods...

...for at least six months. Future directions and obstacles to clinical CRISPR editing Given the success of...diseases to be treated clinically with CRISPR. Many obstacles remain for CRISPR gene editing, but given the ...

...with any integrated transgene). To overcome this obstacle, a positive selection cassette can be incorporated...use of negative selection largely overcomes this obstacle. Furthermore, we have found that transfecting ...