Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 772 results for: LIS

Showing: 181 - 200 of 772 results

  1. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    ...conversation. Goals and Planning Worksheet: Lists accomplishments, research/professional goals and feedback...credit for what we have accomplished. Starting with a recap of accomplishments, from learning new techniques...time has gone. Are we any closer to graduating, publishing that key paper, or figuring out a career path...tangible research and career goals, and celebrate accomplishments. These techniques are laid out in Vincent et...

  2. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...Organizing your lab notebooks, protocols, and to do lists can be easier than you think! Here are three tips...the colors and text. Tip 2: Use electronic to-do lists to access your tasks on the go Never miss a deadline...everything to your calendar. Ditch the paper To Do List and move to one that moves with you. Use software...software such as Evernote or Google Tasks to create lists of your upcoming tasks, set deadlines, and arrange...attachments. Organize your notes into notebooks, create checklists that link to other notes, and use the search ...spreadsheets for reagent storage locations, such as the list used to organize your cryo freezer. No more issues...

  3. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...Jessica Polka from Harvard Medical School and a long list of supporters from all of the research centers in...problems resulting from past practices and mores.  His list is dire:  The current system is unsustainable, hypercompetition...be radically overhauled.  Finally, the science publishing system needs to be changed.  Dr. Bourne charged...training excellence to begin effecting change.  Panelists call for action Each of the speakers in the panel...applause when he talked about how he boycotts publishing in all but open access journals.  Dr. David Glass...astounding lack of reproducibility being recognized in published work even in the “best” journals.  He pushed for...

  4. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    ...we'd like to extend a special thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard...asked leading professors for their top 3 tips. Tom Ellis 1. Keep up with the literature. He recommends setting...too; both with your research and with getting published.” Connie Cepko 1. Work hard. There will be periods...mentees who have goals outside of the university? Ellis recommends that a student interested in leaving ...engineering (STEM) expertise in law, business, journalism, entertainment, politics, K-12, etc.” Addgene's...Acknowledgments Thank you again to Connie Cepko, Tom Ellis, and George Church for answering our burning questions...

  5. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...for Microbiology (ASM) Scientific Writing and Publishing Institute. Writing is the cornerstone of any ... them with the world. If one doesn’t write and publish in some way, then the data produced isn’t likely...manuscripts and the ASM Scientific Writing and Publishing Institute (SWPI) is an extremely beneficial guide...should possibly take a composition class in the English Department. He suggested that I sign up for the...the SWPI online course because a course in the English department wouldn’t have trained me in the scientific...Resources on the Addgene Blog Dos & Don'ts When Publishing a Scientific Manuscript Writing Scientific Manuscripts...

  6. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    ... a neuroscience major, I was struck with the realisation that I didn’t want a career in neuroscience—whatever... to once again write for fun eventually saw an English minor tacked onto my BSc. With this decision I ...towards a career in science communication, an established field of inquiry with which I was not yet familiar...meaningful day job only came into focus when an English tutor noted my eclectic mix of science and arts... a scientific background and bring with them established interests and areas of expertise. Perhaps the... research explores risk-taking behavior among collision sport athletes, as well as the ethical and health...

  7. The Strength of Story Telling

    Type
    Blog Post
    ...magazine. Humans are built to enjoy, remember, and listen to narratives — take advantage of that in your ...stories — whether I’m working with a talented journalist to shine light on living as a scientist in Beijing...research struggles in papers. They recount accomplishments in talks and interviews. They even tell short...tell good stories, they engage their readers or listeners thus expanding the impact and appreciation for...Interviewee: “I did the most work for a paper we were publishing, but when it came time to add author details ...which had affected his judgement. Eventually, we published the paper in a high-impact journal and it worked...

  8. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...she would be looking at where I had published, who I had published with and whether I would be able to... set up an ELISA kit company on whose website you can find some great information on ELISA troubleshooting...Undergraduate sections and brought in more focus on the list of conferences I presented at and placed them under...troubleshooting, ELISA kits and cell death assays. You can find Seán on twitter @Macfhearraigh.    Read more posts...

  9. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ...and foster an effective working environment? Tom Ellis was offered a start-up package that included a postdoc... most surprising thing about becoming a PI? Tom Ellis has a one word answer: “Email. Nothing prepared ...differently or the same as your adviser did? Tom Ellis merges the influence from his two PhD advisers who...who were “chalk-and-cheese”. While one was an established Professor who was always “off flying his plane...our latest results as soon as the gels ran.” Dr. Ellis tries to lead his team as a scientists and a friend... lab!”   Acknowledgments Thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard...

  10. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    ...recent plasmid deposit please sign up here.      Listen to this episode of the Hot Plasmids podcast Light-mediated...transmission Article contributed by Klaus Wanisch  Listen to the optogenetics podcast segment An optogenetic...and joined each fragment with one part of an established pair of proteins (iLID and SspB) that undergo...mammalian hosts Article contributed by Lukas Morgan  Listen to the acoustic reporter gene podcast segment In...regulation Article contributed by Michael Lemieux  Listen to the miRNA reporter podcast segment Discovered... CHIME Article contributed by Aliyah Weinstein  Listen to the CRISPR podcast segment Understanding the...

  11. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...imaging Improving Well-established High-throughput assays Available Well-established Natural and engineered...Commons. The North American firefly, Photinus pyralis, catalyzes a chemical reaction in its abdomen to...systems. Our luciferase collection page has a detailed list of luciferase options. Table 2: Properties of luciferase...Cofactors Secreted North American firefly (Photinus pyralis) Natural or codon-optimized 562 61 Luciferin ATP...Furimazine None Optional AkaLuc(derived from Photinus pyralis) Engineered   677 61 AkaLumine ATP No Common...

  12. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ... their precise positions within the plasmid. The list of enzymes that can be detected is 5x greater than...GenBank File or a SnapGene file for the sequences listed. You can then analyze the sequence in your sequence...Sequence” tabs. Features The table found here lists the common features detected in the plasmid sequence... map, direction (if relevant), and types. Types listed are standard GenBank feature types. Clicking on...Sequence” tabs. Primers This tab contains a table listing commonly used primers detected in a given nucleotide...Analyzer guide A quick guide to all of the tabs listed above and their functions. We hope that these...

  13. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ...pre-clinical life science work, economics) that published research is difficult to replicate.  Why is replicating...appreciate how few details are provided in most published papers. The precise timing, order, or every combination... rarely exist or are preserved as part of the publishing process and become lost if not archived. Remove... article is given “in-principle acceptance” to publish the final results regardless of the outcome of ...null findings that are notoriously difficult to publish.  With Registered Reports, the authors are able...scientific research. These include policies for publishers and funders in the Transparency and Openness ...

  14. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    ... plasmid deposit please sign up here.          Listen to this episode of Hot Plasmids! Optogenetic control...by CASANOVA Article contributed by Cary Valley  Listen to the anti-CRISPR podcast segment Anti-CRISPR ...using blue light (Bubeck et al., 2018). They accomplished this by inserting a photo-sensitive LOV2 domain... Archon Article contributed by Shreya Vedantam  Listen to the voltage indicator podcast segment The neurons... system Article contributed by Michelle Cronin  Listen to the 2in1 cloning podcast segment Recently, the...and AsLOV2 Article contributed by Angela Abitua  Listen to the photoswitchable protein podcast segment ...

  15. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ... Map & Network (CeNGEN) The CeNGEN goal is to establish a comprehensive gene expression atlas of every...publication for the worm community where one can publish brief novel findings. These results are normally...results. This a great resource for scientists to publish findings that don't fit into larger publications... that can be referenced. In addition the data published in microPublications is curated and added to other...but there are Drosophila and zebrafish articles published there as well.   Worm Development Dynamics Database...used for introducing point mutations that was published in a microPublication (Schwartz and Jorgensen,...

  16. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ... impossible to interpret. Polishing the fiber optics with diamond polishing paper of sequentially increasing... second key point; the fiber optics need to be polished to significant smoothness to prevent drastic loss...its output with a light meter before and after polishing. Efficiency of the implant should be at least ... output. The same considerations apply to the polishing and preparation of the patch cable (although you...it’s best to try those that have already been published first. Because behavioral optogenetics is performed...

  17. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    ...intellectual property, it worked away in isolation. It published a paper a year (or less) and limited the exchange...public and to other academic labs. Being unable to publish or attend conferences makes it very challenging...As a Master’s student, she had less pressure to publish and more freedom to attend conferences, but after...occasionally thinking about how we could bypass the establishment hiring system altogether. We had a lot of ideas...restricted to science jobs. Keira’s an optimist, I’m a realist, and together we make a pretty good team. We have... – although we didn’t figure out how we would accomplish the color change until a few months later. RevBio...

  18. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...access to a list of posters until the day-of, take a moment to step aside to read through the list at the ... a day in advance. Dress for the venue and professionalism of the conference, and remember to wear comfortable...about the relationship of your work to recently published papers or “hot topics,” so be sure to be well-...strategically use your time. Don’t have access to a list of posters at all? No problem! At the beginning ...traverse the sea of posters If you come prepared with a list of posters of interest, then you’ve already set ...

  19. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ... thanks to our guest bloggers Alissa Lance-Byrne and Alex Chavez! Alissa Lance-Byrne is a graduate student...This post was contributed by guest bloggers Alissa Lance-Byrne and Alex Chavez, researchers at the Wyss...gene or introduce a desired genetic alteration. Listen to Our Podcast Interview with Alex Chavez ... induce Cas9 to make edits (AC, WLC, and JQ, unpublished results). An additional benefit of the use of...PMC4265475. Additional Resources on the Addgene Blog Listen to Our Interview with Alex Chavez Learn about Sequencing...

  20. Academic vs. Industry Postdocs

    Type
    Blog Post
    ... can be helpful. Publishing: As a postdoc at AstraZeneca, you are expected to publish, but this can vary...prerequisite “industry experience” that I’ve seen listed as a requirement on some industry job postings....vary depending on which company you choose. If publishing is important to you, make sure that your project... Regeneron, and Genentech, among others, and the list is growing. Some post positions as they come up,... Novartis, have specific web sites for postdoc listings, so the method of posting varies between companies...
Showing: 181 - 200 of 772 results