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We narrowed to 545 results for: SEC

Showing: 181 - 200 of 545 results

  2. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ... family, he did not seem much different than my second son of similar age. When he came to visit us and...with Addgene and join us in the fight against FOP. Second, Addgene will be hosting the AJ’s Boston League...

  3. Viral Vectors 101: Transductions

    Type
    Blog Post
    ...for the transduction step. Viral particles are secreted into the cellular media, making viral collection... of cell or organism context you are targeting? Second, what is the biological outcome you want to achieve...

  4. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...a stronger promoter and different biochemical secondary energy sources to increase protein yields from...some of the new viral preps from recent months: Second generation GRAB dopamine sensors from Yulong Li...

  7. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ... or in vitro transcribed, and changes in gRNA secondary structure can affect editing efficiency. Gao et...? Let us know how it’s working in the comments section! Read About NgAgo User's First Impressions Here...

  8. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...you have expertise or knowledge. Where do these intersect? Work with resources: Choose books or articles...her mentoring style. Set goals with a mentor: The secret of accountability To make a mentoring relationship...

  9. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ...approaches. It’s very easy to think that the methods section of a paper is the one that you need to spend the...improve the structure and transparency of methods sections, including clear information about reagents, and...

  10. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...microvesicles (approx. 40 - 150 nm diameter) that are secreted from the cellular membrane into the culture medium...AAVs (Schiller et al., 2018). Exosomal vesicles secreted from producer cells also contain cellular macromolecules...

  12. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ..., examining relationships between microbes and insects, mammals, and plants, among others. I particularly...conclusion? In this series, there is always another secret."     - Marcy Patrick, Associate Director of Quality...

  13. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ... chemogenetic inhibition Flp expression for intersectional studies And more! Control AAV We’re always...that can be used in combination with Cre for intersectional control of specific neuronal populations. We...

  16. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    ...Weigert, later expanded on Gram’s discovery, adding a second stain, safranin, as a counterstain to help visualize...smear with the safranin counterstain for 30 - 60 seconds. Rinse the slide in tap water until the runoff ...

  18. How to Lead a Great Meeting

    Type
    Blog Post
    ...Meetings: For standing team meetings, provide agenda sections for each person to add a bullet or two about what...out the action items. Move them into a separate section at the top of the agenda, and assign each one using...

  19. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...How can protein function be dissected while minimizing confounding secondary effects? An approach described...purification and have been tested in bacterial, insect and mammalian cells. These vectors were designed...
Showing: 181 - 200 of 545 results