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Showing: 1 - 10 of 10 results

  1. Antibody Guide

    Type
    Guide
    ...similar factors. Secondary antibodies Secondary antibodies, often called secondaries, are usually much...and a conjugated secondary antibody specific to your primary antibody. Multiple secondary antibodies will...if your fluorophore is bound to a secondary antibody, the secondary antibodies must be able to distinguish...Therefore, secondary antibodies are species specific and are often referred to by [secondary source] [primary...[primary source secondary recognizes], e.g. , “goat anti-rabbit.” Choose a secondary that recognizes your...applications, and is covered in the following Isotypes section. The variable region of the antibody is the antigen...the amplification of signals by having multiple secondary antibodies binding to a primary antibody which...

  2. Lentiviral Vector Guide

    Type
    Guide
    ... plasmids. Created with BioRender.com. Second-generation Second-generation lentiviral plasmids greatly...contains env All second-generation lentiviral transfer plasmids must be used with a second-generation packaging... either second- or third-generation packaging plasmids. For a summarized comparison of second- and third-generation... Feature Second-generation Third-generation Transfer Plasmid Can be packaged ONLY by a second-generation...thanks to the design of second- and third-generation lentiviral vectors. Second- and third-generation vectors...vector production by removing the accessory genes. Second-generation plasmids include tat , as the 5’ LTR... plasmid, and this requires Tat for activation. Second-generation plasmids include (Figure 3): Transfer...

  3. Adenovirus Guide

    Type
    Guide
    ...vectors. Created with BioRender.com. Second-generation rAdV vectors Second-generation rAdV vectors contain ... is, by engineered packaging HEK293 cell lines. Second-generation rAdV vectors were shown to elicit less...first- and third-generation rAdV vectors. Figure 3: Second-generation rAdV vectors. Created with BioRender.com... vectors is normally transient and short-lived. Second- and third-generation rAdV vectors have been developed...number indicates the serotype of the genome and the second number the serotype of the capsid. Use of these...rAAV vectors (~4.7 kb) and is further increased in second and third-generation vectors. This capacity is ...

  4. Optogenetics Guide

    Type
    Guide
    ...periods. These can range from msec (hChR2) to “long lasting,” e.g. seconds to minutes with stable step-...Zhang F, Bamberg E, Nagel G, Deisseroth K. 2005. Millisecond-timescale, genetically targeted optical control...

  5. CRISPR Guide

    Type
    Guide
    ...CRISPR in your own research. CRISPR Basics In this section, we will introduce the basic mechanisms of CRISPR...Generating a Knockout Using CRISPR Cas9 undergoes a second conformational change upon target binding. In this...have been developed, including editors based on a second ADAR variant that displays improved specificity...Oost, J. (2012). CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled...

  6. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...cell lines, such as HeLa and A549 cells, and even insect Sf9 cells , have also been developed. The newly...number indicates the serotype of the genome and the second number the serotype of the capsid. Pseudotyped ... splice donor sequence at its 3' end, while the second vector carries the 3' portion of the gene with ...

  7. Addgene's eBook Collection

    Type
    Guide
    ...101 July 2024 | Second Edition About Viral Vectors 101 July 2024 | Second Edition About This guide...

  8. Cloning

    Type
    Guide
    ...preserve the directionality of the cloning reaction. Second, the restriction site is encoded on both the insert... DNA that ends in sequences that form complex secondary structues. You can view an example of a LIC protocol...

  9. Plan Your Experiment

    Type
    Guide
    ... manipulate the genome like never before. This section will provide a general framework to get you started...

  10. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...packaging cell line. For example, Phoenix™ is a popular second-generation gamma-retroviral packaging cell line...
Showing: 1 - 10 of 10 results